数字农科院2.0

Ribosomal protein L27 contributes to virulence and maduramicin resistance in Eimeria tenella

文献类型： 外文期刊

作者： Yu Yu;Guo Huilin;Liujia Li;Guiquan Guan;Qiping Zhao;Shunhai Zhu;Jinwen Wang;Fanghe Zhao;Hui Dong;Hongyu Han

作者机构：

关键词： Drug resistance;Eimeria tenella;Maduramicin;Ribosomal protein L27;Virulence

期刊名称： International Journal for Parasitology: Drugs and Drug Resistance

ISSN： 2211-3207

年卷期： 2025 年 29 卷

页码：

收录情况： SCIE（2025版）

摘要： Coccidiosis, which is primarily caused by Eimeria spp., poses a persistent challenge to poultry health and production worldwide. The emergence and spread of drug-resistant strains have significantly compromised the efficacy of anticoccidial therapies. We previously used RNA-seq to demonstrate that ribosomal protein L27 is differentially expressed in drug-sensitive and maduramicin-resistant strains of E. tenella (EtRPL27). In the present study, an RT–qPCR analysis showed that its expression is stage-specific, with the highest levels in sporozoites and second-generation merozoites. Immunofluorescence revealed both the cytoplasmic and partial surface localization of EtRPL27. Notably, EtRPL27 transcript levels were significantly elevated in a maduramicin-resistant strain relative to the drug-sensitive strain. Functional assays showed that anti-EtRPL27 antibodies inhibited the invasion of sporozoites, whereas the transgenic overexpression of EtRPL27 enhanced both the invasion efficiency and pathogenicity of E. tenella in chickens. Importantly, EtRPL27 overexpression reduced its sensitivity to maduramicin, evident as increased oocyst output and a higher anticoccidial index. Resistance classification based on standard indices confirmed moderate-to-high resistance levels in the EtRPL27-overexpressing strain. These findings demonstrate that EtRPL27 is closely associated with both virulence and maduramicin resistance in E. tenella, indicating its potential utility as a molecular marker and therapeutic target.

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