数字农科院2.0

Characterization Of Nucleocytoplasmic Shuttling Of Pseudorabies Virus Protein Ul46

文献类型： 外文期刊

作者： Xu, JJ; Gao, F; Wu, JQ; Zheng, H; Tong, W; Cheng, XF; Liu, YT; Zhu, HJ; Fu, XL; Jiang, YF; Li, LW; Kong, N; Li, GX; Tong, GZ

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关键词： Pseudorabies Virus; Vp11; 12; Nucleocytoplasmic Shuttling; Nuclear Localization Signal; Vp16; Ep0; Sting

期刊名称： FRONTIERS IN VETERINARY SCIENCE

ISSN：

年卷期： 2020 年 7 卷

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收录情况： JCR（2021版）

摘要： Pseudorabies virus (PRV) is the etiological agent of Aujeszky's disease, which has caused severe economic loss in China since its re-emergence in 2011. UL46, a late gene of herpesvirus, codes for the abundant but non-essential viral phosphoproteins 11 and 12 (VP11/12). In this study, VP11/12 was found to localize inside both the nucleus and cytoplasm. The nuclear localization signal (NLS) of VP11/12 was identified as(3)RRARGTRRASWKDASR(18). Further research identified alpha 5 and alpha 7 to be the receptors for NLS and the chromosome region maintenance 1 (CRM1) to be the receptor for the nuclear export signal. Moreover, we found that PRV VP11/12 interacts with EP0 and the stimulator of interferon genes protein (STING), whereas the NLS of VP11/12 is the important part for VP11/12 to interact with UL48. To our knowledge, this is the first study to provide reliable evidence verifying the nuclear localization of VP11/12 and its role as an additional shuttling tegument protein for PRV. In addition, this is also the first study to elucidate the interactions between PRV VP11/12 and EP0 as well as between PRV VP11/12 and STING, while identifying the precise interaction sites of PRV VP11/12 and VP16.

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