数字农科院2.0

An enzyme-encapsulated nanoliposomes based colorimetric immunosensor for the sensitive detection of clothianidin

文献类型： 外文期刊

作者： He Chen;Juan Zhou;Li An;Huan Ma;Meng Li;Haixia Lei;Lei Wang;Lufei Zheng;Zenglong Chen;Xujin Wu

作者机构：

关键词： clothianidin;colorimetric;immunosensor;Nanoliposome;on-site detection

期刊名称： Microchemical Journal

ISSN： 0026-265X

年卷期： 2025 年 219 卷

页码：

收录情况： SCIE（2025版）

摘要： Clothianidin is significant toxicity to non-target organisms, underscoring the necessity for a simple and highly sensitive biosensor for on-site detection. Herein, a pH responsive biotin-tagged horseradish peroxidase (HRP)-encapsulated nanoliposome (HRP@liposome) was synthesized and applied to a colorimetric immunosensor as signal amplification generator. In the presence of clothianidin, it competes with coated antigens for binding to biotinylated antibodies, thereby reducing the number of HRP@liposome due to biotin-streptavidin-biotin interactions. Upon addition of substrate solution, the HRP@liposome are lysed, releasing a substantial amount of HRP, which catalyzes the oxidation of substrate. Under optimal conditions, the colorimetric immunosensor exhibited a 50% inhibition concentration of 4.75 ng mL−1, with a limit of detection of 0.24 ng mL−1, and a linear range of 0.24–66.23 ng mL−1, respectively. The average recoveries were 86.1%–103.6% in spiked environmental and food samples. Compared with conventional enzyme-linked immunosorbent assays, the proposed immunosensor demonstrates higher sensitivity. This research provides a promising reference for rapid assessment in environmental health and food safety.

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