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B-cell epitope mapping of VP5 protein of bluetongue virus serotype 1 using monoclonal antibodies

文献类型： 外文期刊

作者： Fanhua Meng;Xuechun Liu;Yuqing Song;Xinbing Hu;Zhancheng Tian;Guiquan Guan;Lijie Tang;Hong Yin;Junzheng Du

作者机构：

关键词： B-cell epitope;Bluetongue virus serotype 1;Monoclonal antibody;VP5Δ1–79aa protein

期刊名称： Virus Research

ISSN： 0168-1702

年卷期： 2025 年 362 卷

页码：

收录情况： SCIE（2025版）

摘要： Bluetongue (BT) is a severe infectious disease affecting ruminants, caused by the bluetongue virus (BTV), an Orbivirus transmitted by Culicoides midges. VP5 is a major component of the outer capsid of BTV, and a key constituent of BTV subunit and virus-like particle vaccines. However, to date, the B-cell epitopes on VP5 recognized by humoral immune responses remain unidentified. In this study, recombinant VP5Δ1–79aa was expressed in Escherichia coli (E. coli) and purified for mouse immunization. Five monoclonal antibodies (mAbs) were identified through hybridoma fusion, clonal selection, and immunological assays. B-cell epitopes were recognized by mAbs 4G9, 6C9, 3A1, 3C8 and 6B1, these epitopes were mapped using a series of truncated overlapping peptides expressed as glutathione S-transferase fusion proteins. 4G9 recognized the 144DEKQFDILNK153 sequence, 6C9 recognized 154AVTSYNKILT163, 3A1 and 3C8 recognized 207VERDGMQEEA216, and 6B1 recognized 312ENHKELMHIK321. These specific mAbs and their corresponding B-cell epitopes provide valuable insights into the structure and function of VP5, contributing to the advancement of serological diagnostic methods and development of epitope-based vaccines for BTV.

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