数字农科院2.0

Rapid identification of escolar (Lepidocybium flavobrunneum) and oilfish (Ruvettus pretiosus) species based on recombinase polymerase amplification

文献类型： 外文期刊

作者： Xie R.; Zhang J.; Wen S.; Zhao R.; Wang N.; Chen A.

作者机构：

期刊名称： Food Control

ISSN： 0956-7135

年卷期： 2023 年 144 卷

页码：

收录情况： SCIE（2023版）

摘要： Escolar or oilfish being sold as cod is a worldwide concerning food counterfeiting issue. This study established a simple and rapid real-time recombinase polymerase amplification (RPA) assay for oilfish (Ruvettus pretiosus) and escolar (Lepidocybium flavobrunneum) detection. The assay uses RPA primers and a probe designed for the intraspecific conserved and interspecific regions in the COI and Cyt b genes, respectively. The RPA assay was completed within 20 min at 37 °C, was specific for either oilfish or escolar species, and showed no cross-reactivity with other cod or salmon fishes. The limit of detection for oilfish and escolar was 9.6 pg/μL and 48 pg/μL, respectively. Samples that were cooked or digested retained a detection sensitivity of 1%. This real-time RPA assay is a simple, rapid, reliable, and sensitive method for oilfish or escolar identification which may help control oilfish or escolar adulteration and ensure consumer health. © 2022 Elsevier Ltd

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