数字农科院2.0

Attenuation and altered replication of IBV strain tl/CH/LDT3/03 after serial passage in chicken embryo fibroblasts and Vero cells

文献类型： 外文期刊

作者： Huixin Li;Yaping Qin;Zongxi Han;Shengwang Liu

作者机构：

关键词： Cell-adaptation;Chicken embryo fibroblasts;In ovo vaccination;Infectious bronchitis virus;Vero cells

期刊名称： Virus Research

ISSN： 1872-7492

年卷期： 2025 年 361 卷

页码：

收录情况： SCIE（2025版）

摘要： To adapt the infectious bronchitis virus (IBV) for cell culture, the tl/CH/LDT3/03 strain was subjected to serial passaging in chicken embryo fibroblasts (CEFs) and Vero cells, respectively. Cytopathic effects (CPEs) first became apparent at the 7th passage in CEFs and the 11th passage in Vero cells, respectively. The tl/CH/LDT3/03 strain achieved stable replication and adaptation after 20 passages in CEFs (CEA P20) and 25 passages in Vero cells (VEA P25). Analysis of the genomic sequences of the two adapted viruses identified amino acid substitutions, insertions, and deletions in some of the viral proteins. To evaluate the replication capacity of the cell-adapted viruses, 1-day-old SPF chicks were inoculated with either CEA P20 or VEA P25. Both CEA P20 and VEA P25 exhibited reduced replication capacity in chickens, as determined by viral titration in 11 selected tissues collected at 5 days post-inoculation (dpi). The pathogenicity of the two viruses was also decreased for 1-day-old chicks. Furthermore, VEA P25 elicited significantly reduced neutralizing antibody responses in infected birds, nearly 3-fold lower than that induced by CEA P20. To evaluate protective efficacy for chickens, in ovo vaccination of SPF eggs with either CEA P20 or VEA P25 was carried out. Both cell-adapted viruses provided complete protection against tl/CH/LDT3/03 challenge, therefore represent promising attenuated live vaccine candidates for in ovo vaccination. In conclusion, serial propagation of IBV tl/CH/LDT3/03 in CEFs and Vero cells resulted in successful viral adaptation, which was associated with decreased replication capacity and a consequent attenuation of virulence in chickens, showing the potential of live vaccine candidates against tl/CH/LDT3/03.

分类号：

  • 相关文献

[1]Immunogenicity and protective efficacy in monkeys of purified inactivated Vero-cell SARS vaccine. Qin, E,Shi, HY,Tang, L,Wang, C,Chang, GH,Ding, ZF,Zhao, K,Wang, J,Chen, Z,Yu, M,Si, BY,Liu, JY,Wu, DL,Cheng, XJ,Yang, BA,Peng, WM,Meng, QW,Liu, BH,Han, WG,Yin, XA,Duan, HY,Zhan, DW,Tian, L,Li, SL,Wu, JS,Tan, G,Li, Y,Li, YC,Liu, YG,Liu, H,Lv, FS,Zhang, Y,Kong, XG,Fan, BC,Jiang, T,Xu, SL,Wang, XM,Li, CW,Wu, XH,Deng, YQ,Zhao, M,Zhu, QY.

[2]Apoptosis Induced by Duck Reovirus p10.8 Protein in Primary Duck Embryonated Fibroblast and Vero E6 Cells. Geng, Hongwei,Zhang, Yun,Guo, Dongchun,Wang, Yu,Liu, Ming,Zhang, Yun,Liu-Partanen, Yin,Tong, Guangzhi.

[3]RNA sequencing reveals CircRNA expression profiles in chicken embryo fibroblasts infected with velogenic Newcastle disease virus. Libin Chen,Jiayu Ruan,Yiyi Chen,Wenxuan Deng,Jinyu Lai,Lei Fan,Juncheng Cai,Chan Ding,Qiuyan Lin,Bin Xiang,Tao Ren. 2023

[4]Identification of Embryonic Chicken Proteases Activating Newcastle Disease Virus and Their Roles in the Pathogenicity of Virus Used as In Ovo Vaccine. Feng, Helong,Yao, Lun,Zeng, Zhe,Jiang, Liren,Shang, Yu,Wang, Hongcai,Li, Li,Wang, Zichen,Wang, Xin,Yang, Hongchun,Zhao, Qingqing,Ren, Xiangfei,Zhang, Tengfei,Zhang, Rongrong,Guo, Yunqing,Lu, Qin,Hu, Qiao,Zhang, Wenting,Ding, Chan,Shao, Huabin,Cheng, Guofu,Luo, Qingping,Wen, Guoyuan. 2023

[5]How to Break through the Bottlenecks of in Ovo Vaccination in Poultry Farming. Li X.,Liu X.,Cui L.,Liu Z.,Zhang Y.,Li H.. 2024

[6]Lead affects the proliferation and apoptosis of chicken embryo fibroblasts by disrupting intracellular calcium homeostasis and mitochondrial function. Zheng Liu,Budan Liu,Weijun Guan,Hui Wang. 2025

[7]Emergence of novel nephropathogenic infectious bronchitis viruses currently circulating in Chinese chicken flocks. Xu, Qianqian,Han, Zongxi,Wang, Qiuling,Zhang, Tingting,Gao, Mengying,Zhao, Yan,Shao, Yuhao,Li, Huixin,Kong, Xiangang,Liu, Shengwang.

[8]Infectious bronchitis virus nucleoprotein specific CTL response is generated prior to serum IgG. Liu, Guangliang,Wang, Qun,Liu, Nihong,Xiao, Yihong,Tong, Tiegang,Liu, Shengwang,Wu, Donglai. 2012

[9]Co-infection of H9N2 subtype avian influenza virus and infectious bronchitis virus decreases SP-A expression level in chickens. Huang, Qi,Wang, Kai,Pan, Ling,Qi, Kezong,Liu, Hongmei,Huang, Qi,Wang, Kai,Chen, Hongjun.

[10]Recombinant Infectious Bronchitis Coronavirus H120 With The Spike Protein S1 Gene Of The Nephropathogenic Ibyz Strain Remains Attenuated But Induces Protective Immunity. Gao, Mingyan,Yu, Yan,Zhao, Xiumei,Cheng, Xu,Zhou, Sheng,Jiang, Yi. 2020

[11]Development and characterization of a recombinant infectious bronchitis virus expressing the ectodomain region of S1 gene of H120 strain. Wei, Yan-Quan,Guo, Hui-Chen,Dong, Hu,Wang, Hai-Ming,Xu, Jin,Sun, De-Hui,Cai, Xue-Peng,Liu, Ding-Xiang,Sun, Shi-Qi,Liu, Ding-Xiang,Fang, Shou-Guo.

[12]Prediction and identification of novel IBV S1 protein derived CTL epitopes in chicken. Tan, Lei,Liao, Ying,Fan, Jin,Zhang, Yuqiang,Mao, Xiang,Sun, Yingjie,Song, Cuiping,Qiu, Xusheng,Meng, Chunchun,Ding, Chan,Ding, Chan.

[13]Infectious bronchitis virus poly-epitope-based vaccine protects chickens from acute infection. Tan, Lei,Zhang, Yuqiang,Liu, Fang,Yuan, Yanmei,Zhan, Yuan,Sun, Yingjie,Qiu, Xusheng,Meng, Chunchun,Song, Cuiping,Ding, Chan,Ding, Chan.

[14]Protection of chickens against infectious bronchitis by a recombinant fowlpox virus co-expressing IBV-S1 and chicken IFN gamma. Wang, Yun-Feng,Sun, Yong-Ke,Tian, Zhan-Cheng,Shi, Xing-Ming,Tong, Guang-Zhi,Liu, Sheng-Wang,Zhi, Hai-Dong,Kong, Xian-Gang,Wang, Mei. 2009

[15]Transcriptome analysis of chicken kidney tissues following coronavirus avian infectious bronchitis virus infection. Cong, Feng,Liu, Xiaoli,Han, Zongxi,Shao, Yuhao,Kong, Xiangang,Liu, Shengwang. 2013

[16]Recombinant duck enteritis viruses expressing major structural proteins of the infectious bronchitis virus provide protection against infectious bronchitis in chickens. Li, Huixin,Wang, Yulong,Han, Zongxi,Wang, Yu,Liang, Shulin,Jiang, Lu,Kong, Xiangang,Liu, Shengwang,Wang, Yulong,Hu, Yonghao.

[17]Evaluation of recombinant fowlpox virus expressing infectious bronchitis virus S1 gene and chicken interferon-gamma gene for immune protection against heterologous strains. Shi, Xing-Ming,Zhao, Yan,Gao, Hong-Bo,Jing, Zhang,Wang, Mei,Cui, Hong-Yu,Tong, Gang-Zhi,Wang, Yun-Feng. 2011

[18]Recombinant Newcastle disease virus expressing the infectious bronchitis virus S1 gene protects chickens against Newcastle disease virus and infectious bronchitis virus challenge. Zhao, Ran,Sun, Junfeng,Qi, Tianming,Zhao, Wen,Han, Zongxi,Liu, Shengwang,Zhao, Ran,Yang, Xiaopu,Liu, Shengwang.

[19]Differential modulation of avian beta-defensin and Toll-like receptor expression in chickens infected with infectious bronchitis virus. Xu, Yang,Zhang, Tingting,Xu, Qianqian,Liang, Shuling,Ma, Deying,Han, Zongxi,Shao, Yuhao,Liu, Shengwang.

[20]Proteomics analysis of differentially expressed proteins in chicken trachea and kidney after infection with the highly virulent and attenuated coronavirus infectious bronchitis virus in vivo. Cao, Zhongzan,Han, Zongxi,Shao, Yuhao,Liu, Xiaoli,Sun, Junfeng,Yu, Demin,Kong, Xiangang,Liu, Shengwang,Cao, Zhongzan. 2012

作者其他论文 更多>>