数字农科院2.0

Fitness evaluation of a methyl eugenol (ME)-low sensitive strain of Bactrocera dorsalis generated by disruption of the olfactory receptor BdorOR94b1

文献类型： 外文期刊

作者： Yang, Yan;Zhang, Jie;Zhou, Hongxu;Wang, Guirong;Liu, Wei

作者机构：

关键词： （0-1-2）oriental fruit fly;CRISPR/Cas9;male annihilation technique;Sterile Insect Technique (SIT);integrated pest management (IPM);life table analysis;mating competitiveness;olfactory behavior

期刊名称： ENTOMOLOGIA GENERALIS

ISSN： 0171-8177

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： The integration of the Male Annihilation Technique (MAT) and the Sterile Insect Technique (SIT) offers effective pest control strategies but faces challenges due to the vulnerability of sterile males to MAT. Researchers have made significant efforts to reduce male response to ME through time-consuming breeding approaches, such as genetic selection and odor-exposure-driven methods, to develop ME non-responsive strains. By leveraging the chemosensory response of B. dorsalis to ME, a stable strain with reduced sensitivity to ME could be developed by genetically disrupting its key olfactory receptor. Here, we evaluate the fitness of an ME-low-sensitivity strain, previously obtained by genetically knocking out its core olfactory receptor, BdorOR94b1, under laboratory conditions. Life table analysis demonstrated that the BdorOR94b1-/- line completed its life cycle normally, with no significant differences in most of features, except a slightly reduced fecundity and lifespan in mutant females. BdorOR94b1 knockout significantly reduced antennal and behavioral responses to ME, while responses to other relevant compounds and overall olfactory function remained largely intact. Mutant males formed leks and had comparable mating success rates under non-choice conditions, but their competitiveness decreased steadily under female-choice conditions, achieving 30%-34% success against wild-type males. These findings indicate that ME-low-sensitivity strains with disrupted BdorOR94b1 maintain population viability and general olfactory function, showing potential for SIT-MAT applications. Future research should focus on developing genetic sexing strains, mass-rearing protocols, and testing in semi-field to ensure effective deployment in integrated pest management programs.

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