Isolation and Purification of Psoralen and Bergapten from Ficus carica L. Leaves by High-Speed Countercurrent Chromatography
文献类型： 外文期刊
作者： Ito, Yoichiro;Chi Chunyan;Shi Bo;Liang Ping;Li Jingmei
作者机构：
关键词： Bergapten;Ficus carica L leaves;High-speed countercurrent chromatography
期刊名称： JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
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年卷期：
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收录情况： SCI
摘要： High-speed countercurrent chromatography (HSCCC) was successfully applied for the first time for the isolation and purification of psoralen and bergapten from the plant Ficus carica L leaves. The crude extract was obtained by extraction with light petroleum (b.p.: 60degC-90degC) from the dried leaves of Ficus carica L. M-Hexane-ethyl acetate-methanol-water (1:1:1:1, v/v) was used as the two-phase solvent system. Each peak fraction was analyzed by high performance liquid chromatography (HPLC). The method yielded 4.4 mg of psoralen at 99.1% purity and 2.1 mg of bergapten at 98.2% purity from 400 mg of the crude extract in a single run. The structures of the two compounds were identified by 'H-NMR, 13C-NMR, and MS.
分类号： O65
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