数字农科院2.0

Asp 52 and Asp 60 in Paracidovorax citrulli HrpG Are Essential for Transcriptional Activation and Hypersensitive Response Induction

文献类型： 外文期刊

作者： Pei Qiao;Mei Zhao;Lulu Cai;Bo Liu;Chengliang Wang;Wei Guan;Yuwen Yang;Wenjun Zhao;Tingchang Zhao

作者机构：

关键词： aspartic acid;HrpG;hypersensitive response;T3SS;transcription

期刊名称： Horticulturae

ISSN：

年卷期： 2026 年 12 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： Pathogenic bacteria utilize a type III secretion system (T3SS) to inject type III effectors (T3Es) into plant cells, suppressing plant immunity and facilitating colonization. Paracidovorax citrulli, the causal agent of bacterial fruit blotch (BFB) of Cucurbitaceae crops, harbors a functional T3SS like many other plant pathogens. The expression of its T3SS and T3Es is regulated by the two-component system response regulators HrpG and HrpX. Here, we demonstrate that the aspartic acid (Asp) residues at positions 52 and 60 in P. citrulli HrpG are essential for its complete function. Plasmid-mediated complementation of the ΔhrpG mutant with hrpG carrying Asp52→alanine (Ala) or Asp60→Ala mutations failed to restore the ability of P. citrulli to induce a hypersensitive response (HR) in tobacco, whereas the Asp46→Ala mutation fully rescued this phenotype. Furthermore, genomic hrpG point mutations generating strains Aac5 (D52A) and Aac5 (D60A) abolish the activation of hrpX transcription, resulting in decreased HrpX accumulation. Collectively, Asp 52 and Asp 60 in P. citrulli HrpG are essential for transcriptional activation activity of hrpX and HR induction, serving as a potential phosphorylation site (Asp 52) for upstream histidine kinases and a Mg2+ coordination site (Asp 60). Given that conserved Asp residues often function as phosphorylation sites in two-component system response regulators, this study provides a foundation for identifying upstream histidine kinases that modulate HrpG activity in P. citrulli.

分类号：

  • 相关文献

[1]Identification of phenolic compounds that suppress the virulence of Xanthomonas oryzae on rice via the type III secretion system. Tian, Fang,Li, Jianyu,Chen, Huamin,Yang, Fenghuan,He, Chenyang,Hutchins, William,Yuan, Xiaochen,Yang, Ching-Hong,Cui, Zining.

[2]TheDifferentialTranscriptionNetworkbetweenEmbryoandEndospermintheEarlyDevelopingMaizeSeed. XiaoduoLu,DijunChen,DefengShu,ZhaoZhang,WeixuanWang,ChristianKlukas,Ling-lingChen,YunliuFan,MingChen,ChunyiZhang. 2015

[3]西瓜嗜酸菌分泌系统的研究进展. 王铁霖,杨玉文,唐爽爽,刘志恒,赵廷昌. 2014

[4]细菌Ⅲ型分泌系统及其抑制剂研究进展. 陈栋梁,班曼曼,朱梦晗,王霄旸,王春梅,周文,张可煜. 2023

[5]Halonitromethanes Formation From Aspartic Acid in the Presence of Cu2+ During UV254/chloramine Treatment: Experimental and Computational Studies. Khan, Hidayat Ullah,Deng, Lin,Asif, Muhammad,Zhang, Changbo,Singh, Rajendra Prasad,Wu, Gongde. 2025

[6]Involvement Of Hrpx And Hrpg I.n The Virulence Of A cidovorax Citrulli Strain Aac5, Causal Agent Of Bacterial Fruit Blotch In Cucurbits. Yan, Jianpei,Guan, Wei,Yan, Jianpei,Zhao, Tingchang,Zhao, Mei,Yang, Linlin,Yang, Yuwen,Zhang, Xiaoxiao,Walcott, Ron,Yang, Linlin. 2018

[7]Ectopic expression of the cotton non-symbiotic hemoglobin gene GhHb1 triggers defense responses and increases disease tolerance in Arabidopsis. Zhong, Nai-Qin,Wang, Hai-Yun,Chen, An-Ping,Jian, Gui-Liang,Xia, Gui-Xian. 2006

[8]Hrip1, a novel protein elicitor from necrotrophic fungus, Alternaria tenuissima, elicits cell death, expression of defence-related genes and systemic acquired resistance in tobacco. Kulye, Mahesh,Liu, Hua,Zhang, Yuliang,Zeng, Hongmei,Yang, Xiufen,Qiu, Dewen. 2012

[9]Reduction of lesion growth rate of late blight plant disease in transgenic potato expressing harpin protein. Li, RG,Fan, YL.

[10]SPL28 encodes a clathrin-associated adaptor protein complex 1, medium subunit mu 1 (AP1M1) and is responsible for spotted leaf and early senescence in rice (Oryza sativa). Qiao, Yongli,Jiang, Wenzhu,Lee, JooHyun,Park, BongSoo,Choi, Min-Seon,Piao, Rihua,Woo, Mi-Ok,Paek, Nam-Chon,Seo, Hak Soo,Koh, Hee-Jong,Qiao, Yongli,Jiang, Wenzhu,Lee, JooHyun,Park, BongSoo,Choi, Min-Seon,Piao, Rihua,Woo, Mi-Ok,Paek, Nam-Chon,Seo, Hak Soo,Koh, Hee-Jong,Roh, Jae-Hwan,Han, Longzhi. 2010

[11]Crystal Structure Analysis and the Identification of Distinctive Functional Regions of the Protein Elicitor Mohrip2. Wang, Meifang,Zeng, Hongmei,Qiu, Dewen,Duan, Liangwei,Liu, Xinqi. 2016

[12]Mutational analysis of the Verticillium dahliae protein elicitor PevD1 identifies distinctive regions responsible for hypersensitive response and systemic acquired resistance in tobacco. Liu, Zhipeng,Liu, Wenxian,Zeng, Hongmei,Yang, Xiufen,Guo, Lihua,Qiu, Dewen. 2014

[13]Isolation and Characteristics of the CN Gene, a Tobacco Mosaic Virus Resistance N Gene Homolog, from Tobacco. Zhang, Gai-Yun,Guo, Jia-Ming,Xu, Tong-Wen,Chen, Xue-Ping,Chen, Ming,Li, Lian-Cheng,Xu, Zhao-Shi,Ma, You-Zhi. 2009

[14]Plant ERD2-like proteins function as endoplasmic reticulum luminal protein receptors and participate in programmed cell death during innate immunity. Li, Sizhun,Xie, Ke,Zhang, Qiang,Wang, Yan,Tang, Yang,Liu, Dong,Liu, Yule,Hong, Yiguo,He, Chenyang. 2012

[15]Elicitation of the hypersensitive responses in tabacco by a 10.6 kD proteinaceous elicitor from Phytophthora palmi. Cai, YY,Chen, J. 1999

[16]The purification and characterization of a novel hypersensitive-like response-inducing elicitor from Verticillium dahliae that induces resistance responses in tobacco. Yang, Xiufen,Zeng, Hongmei,Liu, Hua,Zhou, Tingting,Tan, Beibei,Yuan, Jingjing,Guo, Lihua,Qiu, Dewen.

[17]Molecular Variation and Genomic Function of Citrus Vein Enation Virus. Runqiu Dou,Qingqing Huang,Tao Hu,Fengzhe Yu,Hongxia Hu,Yaqin Wang,Xueping Zhou,Yajuan Qian. 2023

[18]Type III secretion system genes hrcJ and hrpE affect virulence, hypersensitive response and biofilm formation of group II strains of Acidovorax citrulli. Wang T.,Huang Q.,An X.,Yang Y.,Guan W.,Zhao T.. 2022

[19]A newly identified glycosyltransferase AsRCOM provides resistance to purple curl leaf disease in agave. Zhiwei Lu,Xiaowan Hou,Zhi Ke,Yanmei Zhang,Zi Ping Yang,Wenzhao Zhou. 2023

[20]Genome-wide identification and characterization of putative effectors in Heterodera schachtii that suppress plant immune response. Yao, Ke,Zhang, Menghan,Xu, Jianjun,Peng, Deliang,Huang, Wenkun,Kong, Ling'an,Liu, Shiming,Li, Guangkuo,Peng, Huan. 2025

作者其他论文 更多>>