数字农科院2.0

dctA, dctB, and dctD contribute to the utilization of C4-dicarboxylates, carbon, nitrogen, as well as virulence in Acidovorax citrulli

文献类型： 外文期刊

作者： Lan Wang;Maihemuti Mijiti;Ayijiamali Abuduzhayier;Tingchang Zhao;Wei Guan;Lina Yang;Yuwen Yang

作者机构：

关键词： Acidovorax citrulli;C4-dicarboxylate transport system;dctA;dctB;dctD

期刊名称： Microbial Pathogenesis

ISSN： 0882-4010

年卷期： 2025 年 205 卷

页码：

收录情况： SCIE（2025版）

摘要： Bacterial fruit blotch (BFB), a destructive bacterial disease triggered by Acidovorax citrulli, affects cucurbit crops like watermelon and melon. The absorption and use of carbon sources are foundational for bacteria to successfully colonize host plants. C4-dicarboxylates are critical carbon and energy substances, and their transport is completed by the C4-dicarboxylate transport system (Dct) which plays an important role in typical bacterial metabolism. However, the role of dct genes have not been determined for A. citrulli. To clarify the biological roles of the Dct system-related genes in A. citrulli, we developed dctA1, dctA2, dctB, and dctD deletion mutants, as well as dctA1A2 double deletion mutant, with their corresponding complementary strains in the A. citrulli wild-type strain Aac5 in this study. The functions of Dct-related genes in A. citrulli were analyzed through phenotype assays, including pathogenicity, C4-dicarboxylates utilization, carbon and nitrogen utilization, biofilm formation, swimming motility, and qRT-PCR analysis. Compared to the wild-type strain, the pathogenicity, utilization of C4-dicarboxylates, growth ability in vivo and in vitro, and seed adhesion ability of the mutant strains were significantly limited, while the biofilm formation ability was significantly improved. Additionally, the utilization of select carbon sources (glucose, maltose, and sucrose) and nitrogen sources ((NH4)2SO4, NH4Cl, CH4N2O, and KNO3) was significantly enhanced. qRT-PCR results demonstrated that the deletion of Dct-related genes resulted in significant downregulation of the expression of T3SS-related genes (hrpG and hrpE), the pili-related genes (pilA and pilN), and some flagellum-related genes (fliC, flhC, and flhD). These findings suggested that Dct-related genes were involved in C4-dicarboxylate utilization, carbon and nitrogen use, and the pathogenicity of A. citrulli.

分类号：

  • 相关文献

[1]固氮斯氏假单胞菌dctB基因在大肠杆菌中异源表达的研究. 李红权,平淑珍,何升,陈明,林敏. 2008

[2]瓜类果斑病菌(Acidovorax citrulli)基因组信号肽预测分析. 严婉荣,闫莎莎,杨玉文,赵廷昌. 2012

[3]Functional analysis of luxR/luxI in Acidovorax citrulli. Wang, T.,Zhao, T.. 2012

[4]Genetic diversity analysis of Acidovorax citrulli in China. Yan, Shasha,Yang, Yuwen,Wang, Tielin,Zhao, Tingchang,Schaad, Norman W..

[5]Quorum-sensing contributes to virulence, twitching motility, seed attachment and biofilm formation in the wild type strain Aac-5 of Acidovorax citrulli. Guan, Wei,Yang, Yuwen,Yan, Wanrong,Sun, Baixin,Zhao, Tingchang,Wang, Tielin,Huang, Qi.

[6]Rapid Discrimination between Groups I and II of Acidovorax citrulli Using a Primer Pair Specific to a pilL Gene. Zhong, Jie,Lin, Zhen-Ya,Ma, Ya-Min,Gao, Bi-Da,Liu, Hui-Qin,Zhao, Ting-Chang,Schaad, Norman W..

[7]A Luxr-Type Regulator, Acrr, Regulates Flagellar Assembly And Contributes To Virulence, Motility, Biofilm Formation, And Growth Ability Of Acidovorax Citrulli. Huang, Qi,Liu, Bo,Zhao, Tingchang,Guan, Wei,Tian, Eryuan,Yang, Yuwen,Wang, Tielin. 2020

[8]Transcriptomic and functional analyses reveal roles of AclR, a luxR-type global regular, in regulating motility and virulence of acidovorax citrulli. Wei Guan,Tielin Wang,Qi Huang,Mei Zhao,Eryuan Tian,Yanfeng Liu,Bo Liu,Yuwen Yang,Tingchang Zhao. 2021

[9]Type III secretion system genes hrcJ and hrpE affect virulence, hypersensitive response and biofilm formation of group II strains of Acidovorax citrulli. Wang T.,Huang Q.,An X.,Yang Y.,Guan W.,Zhao T.. 2022

[10]Development Of A Multiplex Pcr A.ssay Based On The P ila Gene Sequences To Detect Different Types Of Acidovorax Citrulli. Bai, X.,Guan, W.,Walcott, R. R.,Zhao, T. C.,Qiao, P.,Yang, Y. W.,Zhang, L. Q.,Zhao, M.,Yang, Y. W.,Zhang, X. X.. 2019

[11]Hcp of the Type VI Secretion System (T6SS) in Acidovorax citrulli Group II Strain Aac5 Has a Dual Role as a Core Structural Protein and an Effector Protein in Colonization, Growth Ability, Competition, Biofilm Formation, and Ferric Iron Absorption. Nuoya Fei,Weiqin Ji,Linlin Yang,Chunyan Yu,Pei Qiao,Jianpei Yan,Wei Guan,Yuwen Yang,Tingchang Zhao. 2022

[12]ntrC Contributes to Nitrogen Utilization, Stress Tolerance, and Virulence in Acidovorax citrulli. Dehua Liu,Mei Zhao,Pei Qiao,Zhanhong Li,Gong Chen,Wei Guan,Qingrong Bai,Ron Walcott,Yuwen Yang,Tingchang Zhao. 2023

[13]Acidovorax citrulli type III effector AopU interferes with plant immune responses and interacts with a watermelon E3 ubiquitin ligase. Linlin Yang,Mei Zhao,Xiaoxiao Zhang,Jie Jiang,Nuoya Fei,Weiqin Ji,Yunfeng Ye,Wei Guan,Yuwen Yang,Tingchang Zhao. 2023

[14]Comparison of Copper-Tolerance Genes between Different Groups of Acidovorax citrulli. Min Zhang,Mei Zhao,Pei Qiao,Dehua Liu,Qingrong Bai,Wei Guan,Yuwen Yang,Tingchang Zhao. 2024

[15]Evidence for a Functional HipBA Toxin–Antitoxin System in Acidovorax citrulli. Hao Zhang,Mei Zhao,Lulu Cai,Wei Guan,Yuwen Yang,Ron Walcott,Wenjun Zhao,Tingchang Zhao. 2025

[16]Comparative transcriptome analysis reveals defense mechanisms of Bacillus velezensis ZY1 against bacterial fruit blotch in watermelon. Haoyu Wei,Tingchang Zhao,Yushanjiang Maimaiti,Tielin Wang,Sheng Han,Wei Guan,Yuwen Yang. 2025

作者其他论文 更多>>