数字农科院2.0

Analysis of mitochondrial DNA polymorphism in the atpA region and design of simple sequence repeat and sequence characterized amplified region markers for distinguishing AD1, CMS-D2 and CMS-D8 cytoplasms in cotton (Gossypium spp.)

文献类型： 外文期刊

作者： Ding, Jiaqi;孙国清;Li, Quanjiang;Wang, Jing;Li, Caihua;Zhang, Lanlan;Meng, Zhigang;Zhou, Tao;Shi, Ji;Ji, Wei;Liu, Jian;Zhang, Xiao;Zhang, Rui;Guo, Sandui

作者机构：

关键词： atpA;CMS;cotton;mitochondrial genome;SCAR;SNP;SSR

期刊名称： PLANT BREEDING

ISSN： 0179-9541

年卷期： 2022 年

页码：

收录情况： SCIE（2022版）

摘要： Previous studies showed that there were differences among AD1, CMS-D2 and CMS-D8 in the atpA gene region of the mitochondrial genome of cotton, but their detailed DNA sequence differences were unclear. RFLP analysis here showed that the mitochondrial atpA gene existed as two copies of different lengths in each line. Inverse polymerase chain reaction (PCR) and sequencing results revealed that one copy contained an intact atpA gene, while the other copy contained a 3 '-truncated version. In the 3 ' flanking region of the intact atpA gene, an simple sequence repeat (SSR) locus was found, with the sequences in AD1, CMS-D2, and CMS-D8 being (TAA)(7)(TA)(6), (TAA)(3)(TA)(2), and (TAA)(4)(TA)(3), respectively. In the 3 ' flanking region of the 3 '-truncated copy, a 515-bp specific chimeric fragment was found in AD1, while a 555-bp specific chimeric fragment was found in the two CMS lines, and CMS-D2 possessed three single-nucleotide polymorphism (SNP) sites compared with CMS-D8. Furthermore, AD1-specific, CMS-D2-specific and CMS-D8-specific sequence characterized amplified region (SCAR) markers were developed to distinguish the three cytoplasms. This study will be useful for distinguishing genotypes in breeding practices and evolutionary studies of cotton.

分类号：

  • 相关文献

[1]Mitochondrial SCAR and SSR Markers for distinguishing cytoplasmic male sterile lines from their isogenic maintainer lines in cotton. Zhang, Xiao,Meng, Zhigang,Zhou, Tao,Sun, Guoqing,Shi, Ji,Zhang, Rui,Guo, Sandui,Zhang, Xiao,Yu, Yuanhua.

[2]用于鉴定陆地棉“三系”中可育胞质和不育胞质的SCAR和SSR分子标记的开发. 张晓,张锐,史计,孟志刚,郭三堆. 2010

[3]陆地棉胞质雄性不育系与保持系线粒体SCAR分子标记. 张晓,张锐,史计,王远,孙国清,孟志刚,周焘,郭三堆. 2011

[4]8个水稻抗条纹叶枯病分子标记有效性的验证及评价. 张亚东,朱镇,陈涛,赵庆勇,周丽慧,于新,王才林. 2012

[5]玉米免DNA制备单株SSR和SCAR基因分型方法研究. 张玮,谢传晓,朱苏文,刘晓鑫,路明,张世煌,程备久. 2007

[6]源于L1的小麦抗黄矮病基因的特异PCR标记及辅助育种的研究. 张增艳,辛志勇,陈孝,王晓萍,刘景芳,杜丽璞. 2002

[7]猕猴桃性别分子标记在软枣猕猴桃中的通用性验证. 郭丹丹,钟云鹏,方金豹,黄武权,任建杰,齐秀娟. 2019

[8]Molecular mapping of stripe rust resistance gene YrJ22 in Chinese wheat cultivar Jimai 22. Chen, Can,Ma, Chuanxi,He, Zhonghu,Lu, Jialing,Li, Jia,Ren, Yan,Xia, Xianchun,Ren, Yan,He, Zhonghu.

[9]QTL Analysis of Spike Morphological Traits and Plant Height in Winter Wheat (Triticum aestivum L.) Using a High-Density SNP and SSR-Based Linkage Map. Zhai, Huijie,Feng, Zhiyu,Li, Jiang,Liu, Xinye,Ni, Zhongfu,Sun, Qixin,Zhai, Huijie,Feng, Zhiyu,Li, Jiang,Liu, Xinye,Ni, Zhongfu,Sun, Qixin,Xiao, Shihe. 2016

[10]Construction of a high-density genetic linkage map in pear (Pyrus communis x Pyrus pyrifolia nakai) using SSRs and SNPs developed by SLAF-seq. Wang, Long,Wu, Jun,Yin, Hao,Zhang, Shaoling,Wang, Long,Li, Xiugen,Wang, Lei,Xue, Huabai.

[11]PeanutDB: an integrated bioinformatics web portal for Arachis hypogaea transcriptomics. Shu, Changlong,Zhang, Jie,Schmidt, Emily,Li, Pei,Lenox, Douglas,Liu, Lin,Liang, Chun,Schmidt, Emily,Lenox, Douglas,Liang, Chun. 2012

[12]Construction and analysis of a high-density genetic linkage map in cabbage (Brassica oleracea L. var. capitata). Wang, Wanxing,Liu, Yumei,Fang, Zhiyuan,Yang, Limei,Yuan, Suxia,Sun, Jifeng,Zhuang, Mu,Zhang, Yangyong,Zeng, Aisong,Huang, Shunmou,Hua, Wei,Liu, Shengyi. 2012

[13]Comprehensive analysis of expressed sequence tags from cultivated and wild radish (Raphanus spp.). Shen, Di,Qiu, Yang,Li, Xixiang,Shen, Di,Sun, Honghe,Huang, Mingyun,Zheng, Yi,Fei, Zhangjun,Sun, Honghe,Fei, Zhangjun. 2013

[14]Identification of a new stripe rust resistance gene in Chinese winter wheat Zhongmai 175. LU Jia-ling, CHEN Can, LIU Peng, HE Zhong-hu, XIA Xian-chun. 2016

[15]吉林省玉米种质资源SSR和SNP分子身份证的构建及应用. 张茗起,王蕊,张春宵,孙擘,任洁,李淑芳,王璐,朱少喜,张江斌,施昕晨,王海杰,张云龙,田红丽,赵怡锟,匡猛,王元东,易红梅,李晓辉,王凤格. 2024

[16]Characterization of the global transcriptome for cotton (Gossypium hirsutum L.) anther and development of SSR marker. Xianwen Zhang ,Zhenwei Ye,TiankangWang,Hairong Xiong,Xiaoling Yuan,Zhigang Zhang,Youlu Yuan,Zhi Liu.

[17]Identification of SSR markers linked to the abscission of cotton boll traits and mining germplasm in Cotton. Guangling Shui,Hairong Lin,Xiaomei Ma,Bo Zhu,Peng Han,Nurimanguli Aini,Chunping Gou,Yuanlong Wu,Zhenyuan Pan,Chunyuan You,Guoli Song,Xinhui Nie. 2024

[18]Evolutionary dynamics of mitochondrial genomes and intracellular transfers among diploid and allopolyploid cotton species. Kong, Jiali,Wang, Jie,Nie, Liyun,Tembrock, Luke R.,Zou, Changsong,Kan, Shenglong,Ma, Xiongfeng,Wendel, Jonathan F.,Wu, Zhiqiang. 2025

[19]Comparative analysis of mitochondrial atpA 3'-flanking simple sequence repeat (SSR) polymorphisms in diploid and tetraploid cotton (Gossypium spp.). Song, Yan,Ding, Jiaqi,Yan, Yaxin,Wang, Yiqiong,Zhang, Bing,Zhang, Xiao,Zhang, Rui. 2025

[20]Cloning and Expression of the Chloroplast Copper/Zinc-Superoxide Dismutase Gene in Upland Cotton （Gossypium hirsutum L.）. Yu Shuxun,Fan Shuli,Song Meizhen. 2007

作者其他论文 更多>>