数字农科院2.0

Ultra-efficient nucleic acids extraction using solvent-responsive magnetic MXene nanocomposite

文献类型： 外文期刊

作者： Li, Fukai;Wang, Jiale;Li, Kai;Wang, Min;Zhou, Jian;Yang, Mengrui;Zhang, Xiao;Li, Liang

作者机构：

关键词： Nucleic acids;MXene;Magnetic;RT-qPCR;Plant

期刊名称： MICROCHEMICAL JOURNAL

ISSN： 0026-265X

年卷期： 2026 年 220 卷

页码：

收录情况： SCIE（2025版）

摘要： Simple, efficient and mild extraction of nucleic acids (NAs) represents a promising approach for food safety diagnostic analysis. The magnetic beads (MBs) have potential application value in the separation of NAs due to a variety of obvious capabilities. However, the inadequate NA extraction and separation efficiency of conventional MBs restricts their extensive application. Herein, we have developed a rapid and non-elution 2D layered magnetic MXene nanocomposite (Fe3O4@MXene@PDA@FS) for NAs extraction, which is created by aggregating Fe3O4 beads, MXene and fluorocarbon surfactant (FS) to form an integrated structure with solvent-responsive characteristics. The prepared materials exhibit remarkable extraction capabilities in various complex matrices. Furthermore, the whole extraction procedure can be completed within 30 min and detect as few as 10 copies of NA molecules per sample. The method has significant application prospects in DNA/RNA agricultural detection and early monitoring.

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