数字农科院2.0

Positive Regulation of Maize Resistance to Bipolaris maydis by the Cell Death Suppressor ZmDAD1

文献类型： 外文期刊

作者： Ruoya Lv;Hongliang Wu;Haiyue Yu;Na Ning;Chaotian Liu;Ying Ding;Shu Li;Wende Liu;Zhiqiang Li

关键词： defender against cell death 1; DAD1; southern corn leaf blight; Bipolaris maydis; virus-induced gene silencing (VIGS)

期刊名称： PLANT PHYSIOLOGY AND BIOCHEMISTRY

ISSN： 0981-9428

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： 17 Abstract 18 Defender Against Cell Death 1 (DAD1) is a conserved negative regulator of 19 programmed cell death (PCD), with homologs widely present in plants that modulate 20 immune-related cell death. However, the role and underlying mechanism of DAD1 in maize 21 resistance to Bipolaris maydis, which causes southern corn leaf blight (SCLB) remain 22 unclear. Here, the maize DAD1 gene was characterized, and its predicted amino acid 23 sequence contains a conserved oligosaccharyltransferase (OST) subunit domain. 24 Subcellular localization assays showed that ZmDAD1 primarily localizes to the 25 endoplasmic reticulum (ER), aligning with the documented localization of its orthologs in 26 other species and suggesting a conserved functional role. Gene expression analysis 27 showed that ZmDAD1 is significantly induced following B. maydis infection, with its 28 expression peaking at 12 hours post-inoculation (hpi), supporting that ZmDAD1 is related 29 to the early defense response. Further functional validation confirmed that the transient 30 overexpression of ZmDAD1 effectively suppressed cell death in both Nicotiana 31 benthamiana and maize protoplasts. Conversely, Silencing ZmDAD1 through virus- 32 induced gene silencing (VIGS) compromised maize resistance to B. maydis, whereas its 33 transient overexpression enhanced resistance. Protein-Protein interaction predictions 34 suggested that ZmDAD1 may associate with multiple OST complex subunits and ER 35 stress-related proteins. Transcriptome profiling further revealed that ZmDAD1 transient 36 overexpression plants showed significant enrichment of differentially expressed genes 37 involved in glutathione metabolism, glycosyltransferase activity, and cellulose synthase. 38 Collectively, ZmDAD1 plays a crucial role in the maize-B. maydis interaction. It enhances 39 plant immune defense responses by suppressing cell death, providing new insights into 40 the molecular mechanisms underlying disease resistance in maize.

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