数字农科院2.0

Monoculm1 confers cold tolerance at the seedling stage in rice

文献类型： 外文期刊

作者： Ruiqing Li;Chenfan Zheng;Binqiang Wang;Shang Dai;Yue Song;Huali Zhang;Zhiguo E;Bo Liu;Jiale Chen;Can Zhang;Haowei Fu;Ning Xu;Liangyong Ma;Qian Hao Zhu;Vasileios Fotopoulos;Qingyao Shu;Meng Jiang

作者机构：

关键词： cold stress;DREB1G;haplotype;phosphorylation;transcription factor

期刊名称： Molecular Plant

ISSN： 1674-2052

年卷期： 2025 年 19 卷 1 期

页码：

收录情况： SCIE（2025版） ; ; CSCD（2025-2026年度） ; ; 科技核心（2024版）

摘要： Transcriptional regulation of cold-responsive genes plays crucial roles in plant cold tolerance, but the transcription factors (TFs)-centered regulatory networks remain largely unclear. In this study, we show that Monoculm1 (MOC1), a critical TF controlling tiller number and plant height in rice, positively regulates rice cold tolerance at the seedling stage. We found that OsMPK4, a mitogen-activated protein kinase, phosphorylates and stabilizes MOC1 under cold stress. Further investigations revealed that MOC1 recruits the TFs OsbZIP79 and OsNAC5 to form a triple complex and subsequently enhances their stability by inhibiting proteasome-mediated degradation under cold stress. Notably, we found that the OsbZIP79–MOC1–OsNAC5 complex activates several cold-responsive genes, including Dehydration-responsive element-binding factor 1G (OsDREB1G), to confer rice cold tolerance. Haplotype analysis of the OsDREB1G promoter in > 10,000 rice accessions identified the favorable haplotype and key variants that endow rice cold tolerance. Collectively, our work demonstrates a pivotal role of the OsMPK4–OsbZIP79–MOC1–OsNAC5–OsDREB1G module in regulating rice cold tolerance and provides genetic targets for improving cold tolerance through molecular breeding.

分类号：

  • 相关文献

[1]A major gene for chilling tolerance variation in Indica rice codes for a kinase OsCTK1 that phosphorylates multiple substrates under cold. Wu, Jiawen,Liu, Huimin,Zhang, Yan,Zhang, Yingdong,Li, Dongling,Liu, Shiyan,Lu, Shan,Wei, Lihui,Hua, Jian,Zou, Baohong. 2024

[2]Identification of a novel bZIP transcription factor in Camellia sinensis as a negative regulator of freezing tolerance in transgenic arabidopsis. Wang, Lu,Cao, Hongli,Qian, Wenjun,Yao, Lina,Hao, Xinyuan,Li, Nana,Yang, Yajun,Wang, Xinchao,Wang, Lu,Hao, Xinyuan,Yang, Yajun,Wang, Xinchao,Qian, Wenjun.

[3]Differential expression of CYB5A in Chinese and European pig breeds due to genetic variations in the promoter region. Bai, Y.,Zhang, J. B.,Fang, M. Y.,Bai, Y.,Xue, Y.,Peng, Y. L.,Chen, G..

[4]Protein-DNA interactions in the promoter region of the gene encoding diapause hormone and pheromone biosynthesis activating neuropeptide of the cotton bollworm, Helicoverpa armigera. Hong, B,Zhang, ZF,Tang, SM,Yi, YZ,Zhang, TY,Xu, WH.

[5]Comparative analysis of muscle phosphoproteome induced by salt curing. Wang, Zhenyu,Zhang, Caixia,Li, Zheng,Shen, Qingwu,Zhang, Dequan,Shen, Qingwu,Wang, Zhenyu.

[6]Calcium-dependent protein kinase 21 phosphorylates 14-3-3 proteins in response to ABA signaling and salt stress in rice. Chen, Yixing,Zhou, Xiaojin,Chang, Shu,Chu, Zhilin,Wang, Hanmeng,Han, Shengcheng,Wang, Yingdian,Zhou, Xiaojin.

[7]Identification of bovine casein phosphorylation using TiO2 enrichment in combination with nano-ESI-MS/MS. Li, S. S.,Yang, Y. X.,Wang, J. Q.,Bu, D. P.,Wei, H. Y.,Zhang, L. Y.,Zhou, L. Y.. 2010

[8]Effects of phosphorylation on mu-calpain activity at different incubation temperature. Du, Manting,Li, Xin,Li, Zheng,Wang, Ying,Li, Guixia,Zhang, Dequan,Shen, Qingwu.

[9]Identification of in vivo protein phosphorylation sites in human pathogen Schistosoma japonicum by a phosphoproteomic approach. Luo, Rong,Zhou, Chunjing,Lin, Jiaojiao,Yang, Dehao,Shi, Yaojun,Cheng, Guofeng.

[10]Arabidopsis cytosolic glutamine synthetase AtGLN1;1 is a potential substrate of AtCRK3 involved in leaf senescence. Li, RJ,Hua, W,Lu, YT.

[11]Quantitative phosphoproteomic analysis of early seed development in rice (Oryza sativa L.). Qiu, Jiehua,Hou, Yuxuan,Tong, Xiaohong,Wang, Yifeng,Lin, Haiyan,Liu, Qing,Zhang, Wen,Li, Zhiyong,Zhang, Jian,Nallamilli, Babi R..

[12]Phosphorylation inhibits the activity of mu-calpain at different incubation temperatures and Ca2+ concentrations in vitro. Du, Manting,Li, Xin,Li, Zheng,Li, Meng,Gao, Lingling,Zhang, Dequan,Du, Manting.

[13]Identification of Bovine Casein Phosphorylation Using Titanium Dioxide Enrichment in Combination with Nano Electrospray Ionization Tandem Mass Spectrometry. Li Shan-shan,Wang Jia-qi,Wei Hong-yang,Yang Yong-xin,Bu Deng-pan,Zhang Le-ying,Zhou Ling-yun. 2012

[14]Revealing phosphorylation regulatory networks during embryogenesis of honey bee worker and drone (Apis mellifera). Beibei Ma,Chuan Ma,Jianke Li,Yu Fang. 2022

[15]Phosphorylation and acetylation responses of glycolytic enzymes in meat to different chilling rates. Yuqiang Bai,Chi Ren,Chengli Hou,Li Chen,Zhenyu Wang,Xin Li,Dequan Zhang. 2023

[16]BIN2 phosphorylates the Thr280 of CO to restrict its function in promoting Arabidopsis flowering. Ju L.,Dong H.,Yang R.,Jing Y.,Zhang Y.,Liu L.,Zhu Y.,Chen K.-M.,Ping J.,Sun J.. 2023

[17]Soluble starch synthase enzymes in cereals: An updated review. Ahsan Irshad,Huijun Guo,Shoaib Ur Rehman,Xueqing Wang,Chaojie Wang,Ali Raza,Chunyun Zhou,Yuting Li,Luxiang Liu. 2021

[18]Fus3, as a Critical Kinase in MAPK Cascade, Regulates Aflatoxin Biosynthesis by Controlling the Substrate Supply in Aspergillus flavus, Rather than the Cluster Genes Modulation. Longxue Ma,Xu Li,Fuguo Xing,Junning Ma,Xiaoyun Ma,Yiran Jiang. 2022

[19]Exploring the diversity of plant proteome. Yanmei Chen,Yi Wang,Jun Yang,Wenbin Zhou,Shaojun Dai. 2021

[20]The Modulation of Sucrose Nonfermenting 1-Related Protein Kinase 2.6 State by Persulfidation and Phosphorylation: Insights from Molecular Dynamics Simulations. Li, Miaomiao,Wu, Ting,Wang, Shuhan,Duan, Tianqi,Huang, Siqi,Xie, Yanjie. 2023

作者其他论文 更多>>