数字农科院2.0

Natural Variation in the FtNAC2 Promoter RegulatesQuercetin Accumulation and Drought Tolerance inTartary Buckwheat

文献类型： 外文期刊

作者： Jing Wang; Wei Li;Dongqing Fan;Yuqi He;Yaliang Shi; Hao Lin;Marie‐Laure Fauconnier;Giorgia Purcaro;Muriel Quinet ;Manon Genva;Kaixuan Zhang;Mengqi Ding ;Meiliang Zhou

关键词： promoter variation;quercetin;Tartary buckwheat

期刊名称： PLANT CELL AND ENVIRONMENT

ISSN： 0140-7791

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： Tartary buckwheat, valued for its nutritious and medicinal quercetin. Following two independent domestication events, distinctquercetin accumulation patterns have emerged between the southwestern (SL) and northern (NL) landrace populations. However,the genetic mechanisms underlying these metabolic divergences remain elusive. Here, we identified the transcription factorFtNAC2 through genome‐wide association study (GWAS) of quercetin content in 480 accessions of Tartary buckwheat. Haplotypeanalysis identified two single nucleotide polymorphisms (SNPs) in the FtNAC2 promoter that defined three major haplotypes, withhigher promoter activity and gene expression observed in Hap2. Functional characterization revealed that FtNAC2 promotesquercetin accumulation in Tartary buckwheat hairy roots and potentially serves as a multifunctional regulator influencing bothdrought tolerance in buckwheat and seed size in Arabidopsis. Transcriptome co‐clustering and pull‐down mass spectrometry (MS)indicated FtNAC52 as a potential regulatory partner of FtNAC2. DNA affinity purification sequencing (DAP‐seq) and quantitativereverse transcription PCR (qRT‐PCR) analyses demonstrated that FtNAC2 promoted quercetin biosynthesis by upregulatingFtF3'H and FtF3'5'H genes. Collectively, our results elucidated how FtNAC2 influences quercetin content variation in Tartarybuckwheat, providing molecular insights into the differential quercetin accumulation between cultivated populations.

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