数字农科院2.0

Molecular Dissection of the Gene OsGA2ox8 Conferring Osmotic Stress Tolerance in Rice

文献类型： 外文期刊

作者： Yinxiao Wang;Fengping Du;Juan Wang;Yingbo Li;Yue Zhang;Xiuqin Zhao;Tianqing Zheng;Zhikang Li;Jianlong Xu;Wensheng Wang;Binying Fu

作者机构：

关键词： Gibberellin 2-oxidase gene;Jasmonate;Osmotic stress tolerance;Rice

期刊名称： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ISSN： 1422-0067

年卷期： 2021 年 22.0 卷 17.0 期

页码：

收录情况： JCR（2021版）

摘要： Gibberellin 2-oxidase (GA2ox) plays an important role in the GA catabolic pathway and the molecular function of the OsGA2ox genes in plant abiotic stress tolerance remains largely unknown. In this study, we functionally characterized the rice gibberellin 2-oxidase 8 (OsGA2ox8) gene. The OsGA2ox8 protein was localized in the nucleus, cell membrane, and cytoplasm, and was induced in response to various abiotic stresses and phytohormones. The overexpression of OsGA2ox8 significantly enhanced the osmotic stress tolerance of transgenic rice plants by increasing the number of osmotic regulators and antioxidants. OsGA2ox8 was differentially expressed in the shoots and roots to cope with osmotic stress. The plants overexpressing OsGA2ox8 showed reduced lengths of shoots and roots at the seedling stage, but no difference in plant height at the heading stage was observed, which may be due to the interaction of OsGA2ox8 and OsGA20ox1, implying a complex feedback regulation between GA biosynthesis and metabolism in rice. Importantly, OsGA2ox8 was able to indirectly regulate several genes associated with the anthocyanin and flavonoid biosynthetic pathway and the jasmonic acid (JA) and abscisic acid (ABA) biosynthetic pathway, and overexpression of OsGA2ox8 activated JA signal transduction by inhibiting the expression of jasmonate ZIM domain-containing proteins. These results provide a basis for a future understanding of the networks and respective phenotypic effects associated with OsGA2ox8.

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