数字农科院2.0

Identification and Evolution of Salt-Tolerant Genes in Weedy Rice Through High-Throughput Resequencing

文献类型： 外文期刊

作者： Bing Zou;Bing Han;Jianchang Sun;Mingmao Sun;Xiaoding Ma;Li Chen;Di Cui;Jing Ma;Xiaohong Guo;Longzhi Han

作者机构：

关键词： gene knockout;genome-wide association study;Na+ and K+ flux;salt-tolerance genes;salt-tolerance identification;weedy rice

期刊名称： Plant Direct

ISSN： 2475-4455

年卷期： 2025 年 9 卷 9 期

页码：

收录情况： SCIE（2025版）

摘要： Weedy rice, a wild relative of cultivated rice, is highly stress-resistant and proliferates in paddy fields. In this study, 353 weedyrice accessions were analyzed to identify salt-tolerance genes using population evolution analysis, phenotypic screening,genome-wide association studies (GWAS), transcriptome analysis, haplotype characterization, gene knockout experiments, andNa+ and K+ ion flux assays. Population structure analysis classified the accessions into six distinct groups. Three salt-tolerantaccessions—HW131, HW136, and HW119—were identified based on leaf rolling degree (LRD), leaf withering degree (LWD),chlorophyll content (ChlC), and nitrogen content (NC) traits. GWAS and transcriptome data pinpointed LOC_Os06g39270 andLOC_Os06g11860 as candidate salt-tolerance genes. Haplotype analysis and qPCR confirmed two major haplotypes: AHap2and BHap1. A 2-bp deletion (TC) at position 818 bp in LOC_Os06g11860 was associated with severe salt sensitivity (phenotypicgrade 7), whereas the wild-type exhibited strong tolerance (grade1). Knockout mutants exhibited significantly increased Na+ andK+ flux across mesophyll cell membranes compared to wild-type plants, validating LOC_Os06g11860 (OsERFH1) as a crucial salt-tolerance gene. This study provides novel genetic insights into salt-stress adaptation in weedy rice, paving the way for breedingenhanced salt-tolerant varieties.

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