数字农科院2.0

Molecular Cloning And Functional Analysis Of Thenpr1Homolog In Kiwifruit (Actinidia Eriantha)

文献类型： 外文期刊

作者： Sun, LM; Fang, JB; Zhang, M; Qi, XJ; Lin, MM; Chen, JY

作者机构：

关键词： Kiwifruit; Npr1; Disease Resistance; Pseudomonas Syringaepv; Actinidiae; Systemic Acquired Resistance; Salicylic Acid

期刊名称： FRONTIERS IN PLANT SCIENCE

ISSN： 1664-462X

年卷期： 2020 年 11 卷

页码：

收录情况： JCR（2021版）

摘要： Kiwifruit bacterial canker, caused by the bacterial pathogenPseudomonas syringaepv.actinidiae(Psa), is a destructive disease in the kiwifruit industry globally. Consequently, understanding the mechanism of defense against pathogens in kiwifruit could facilitate the development of effective novel protection strategies. TheNon-expressor of Pathogenesis-Related genes 1(NPR1) is a critical component of the salicylic acid (SA)-dependent signaling pathway. Here, a novel kiwifruitNPR1-like gene, designatedAeNPR1a, was isolated by using PCR and rapid amplification of cDNA ends techniques. The full-length cDNA consisted of 1952 base pairs with a 1,746-bp open-reading frame encoding a 582 amino acid protein. Homology analysis showed that the AeNPR1a protein is significantly similar to the VvNPR1 of grape. A 2.0 Kb 5 '-flanking region ofAeNPR1awas isolated, and sequence identification revealed the presence of several putative cis-regulatory elements, including basic elements, defense and stress response elements, and binding sites for WRKY transcription factors. Real-time quantitative PCR results demonstrated thatAeNPR1ahad different expression patterns in various tissues, and its transcription could be induced by phytohormone treatment and Psa inoculation. The yeast two-hybrid assay revealed that AeNPR1a interacts with AeTGA2. Constitutive expression ofAeNPR1ainduced the expression of pathogenesis-related gene in transgenic tobacco plants and enhanced tolerance to bacterial pathogens. In addition,AeNPR1aexpression could restore basal resistance toPseudomonas syringaepv.tomatoDC3000 (Pst) inArabidopsisnpr1-1mutant. Our data suggest thatAeNPR1agene is likely to play a pivotal role in defense responses in kiwifruit.

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