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Foot-And-Mouth Disease Virus 3B Protein Interacts With Pattern Recognition Receptor Rig-I To Block Rig-I-Mediated Immune Signaling And Inhibit Host Antiviral Response

文献类型： 外文期刊

作者： Zhang, XL; Zhu, ZX; Wang, CC; Yang, F; Cao, WJ; Li, PF; Du, XL; Zhao, FR; Liu, XT; Zheng, HX

作者机构：

期刊名称： JOURNAL OF IMMUNOLOGY

ISSN： 0022-1767

年卷期： 2020 年 205 卷 8 期

页码：

收录情况： JCR（2021版）

摘要： Foot-and-mouth disease is a highly contagious disease of pigs, sheep, goats, bovine, and various wild cloven-hoofed animals caused by foot-and-mouth disease virus (FMDV) that has given rise to significant economic loss to global livestock industry. FMDV 3B protein is an important determinant of virulence of the virus. Modifications in 3B protein of FMDV considerably decrease virus yield. In the current study, we demonstrated the significant role of 3B protein in suppression of type I IFN production and host antiviral response in both human embryonic kidney HEK293T cells and porcine kidney PK-15 cells. We found that 3B protein interacted with the viral RNA sensor RIG-I to block RIG-I-mediated immune signaling. 3B protein did not affect the expression of RIG-I but interacted with RIG-I to block the interaction between RIG-I and the E3 ubiquitin ligase TRIM25, which prevented the TRIM25-mediated, Lys63-linked ubiquitination and activation of RIG-I. This inhibition of RIG-I-mediated immune signaling by 3B protein decreased IFN-beta, IFN-stimulated genes, and proinflammatory cytokines expression, which in turn promoted FMDV replication. All of the three nonidentical copies of 3B could inhibit type I IFN production, and the as 17A in each copy of 3B was involved in suppression of IFN-related antiviral response during FMDV infection in porcine cells. Together, our results indicate the role of 3B in suppression of host innate immune response and reveal a novel antagonistic mechanism of FMDV that is mediated by 3B protein.

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