数字农科院2.0

A fast breeding strategy creates fragrance- and anthocyanin-enriched rice lines by marker-free gene-editing and hybridization

文献类型： 外文期刊

作者： Tiantian Shi;Ying Gao;Andi Xu;Rui Wang;Mingjie Lyu;Yinglu Sun;Luoying Chen;Yuanhang Liu;Rong Luo;Huan Wang;Jun Liu

作者机构：

关键词： Anthocyanin;CRISPR/Cas9 edit system;F25 inbred line;Fragrance;Hybridization

期刊名称： Molecular Breeding

ISSN： 1380-3743

年卷期： 2023 年 43 卷 4 期

页码：

收录情况： SCIE（2023版）

摘要： As rice is a staple food for nearly half of the world’s population, rice varieties with excellent agronomic traits as well as high flavor and nutritional quality such as fragrant rice and purple rice are naturally favored by the market. In the current study, we adopt a fast breeding strategy to improve the aroma and anthocyanin content in the excellent rice inbred line, F25. The strategy skillfully used the advantages of obtaining editing pure lines in T0 generation of CRISPR/Cas9 editing system and easy observation of purple character and grain shape, integrated the subsequent screening of non-transgenic lines, and the elimination of undesirable edited variants from gene-editing and cross-breeding at the same time as the separation of the progeny from the purple cross, thus expediting the breeding process. Compared with conventional breeding strategies, this strategy saves about 6–8 generations and reduces breeding costs. Firstly, we edited the OsBADH2 gene associated with rice flavor using an Agrobacterium-mediated CRISPR/Cas9 system to improve the aroma of F25. In the T0 generation, a homozygous OsBADH2-edited F25 line (F25B) containing more of the scented substance 2-AP was obtained. Then, we crossed F25B (♀) with a purple rice inbred line, P351 (♂), with high anthocyanin enrichment to improve the anthocyanin content of F25. After nearly 2.5 years of screening and identification over five generations, the undesirable variation characteristics caused by gene-editing and hybridization and the transgenic components were screened out. Finally, the improved F25 line with highly stable aroma component, 2-AP, increased anthocyanin content and no exogenous transgenic components were obtained. This study not only provides high-quality aromatic anthocyanin rice lines that meet the market demand, but also offers a reference for the comprehensive use of CRISPR/Cas9 editing technology, hybridization, and marker-assisted selection to accelerate multi-trait improvement and breeding process.

分类号：

  • 相关文献

[1]Rapid alterations of gene expression and cytosine methylation in newly synthesized Brassica napus allopolyploids. Xu, Yanhao,Zhong, Lan,Wang, Jianbo,Wu, Xiaoming,Fang, Xiaoping.

[2]Impact of chiral tebuconazole on the flavor components and color attributes of Merlot and Cabernet Sauvignon wines at the enantiomeric level. Zhao S., Li M., Simal-Gandara J.,田健., Chen J., Dai X., Kong Z.. 2022

[3]Impact of chiral tebuconazole on the flavor components and color attributes of Merlot and Cabernet Sauvignon wines at the enantiomeric level. Zhao S., Li M., Simal-Gandara J., Tian J., Chen J., Dai X., Kong Z.. 2022

[4]Pyramiding Rice Blast Resistance Gene Pi2 and Fragrance Gene badh2. Yakun Wang,Shengjia Tang,Naihui Guo,Ruihu An,Zongliang Ren,Shikai Hu,Xiangjin Wei,Guiai Jiao,Lihong Xie,Ling Wang,Ying Chen,Fengli Zhao,Shaoqing Tang,Peisong Hu,Zhonghua Sheng. 2023

[5]Genetic Variation, Polyploidy, Hybridization Influencing the Aroma Profiles of Rosaceae Family. Xi Chen,Yu Zhang,Weihua Tang,Geng Zhang,Yuanhua Wang,Zhiming Yan. 2024

[6]Genetic changes following hybridization and genome doubling in synthetic Brassica napus. Xu, Yanhao,Xu, Hong,Wang, Jianbo,Xu, Yanhao,Wu, Xiaoming,Fang, Xiaoping.

[7]A case study on the genetic origin of the high oleic acid trait through FAD2-1 DNA sequence variation in safflower (Carthamus tinctorius L.). Sara Rapson,Man Wu,Shoko Okada,Alpana Das,Pushkar Shrestha,Xue-Rong Zhou,Craig Wood,Allan Green,Surinder Singh,Qing Liu. 2015

[8]Rapid detection and identification of viroids in the genus Coleviroid using a universal probe. Wu, Zujian,Xie, Lianhui,Jiang, Dongmei,Kang, Ni,Qin, Lu,Li, Shifang,Hou, Wanying,Sano, Teruo. 2013

[9]Somatic cybridization between Nicotiana tabacum and N-repanda based on a single inactivation procedure of nuclear donor parental protoplasts. Sun, YH,Xue, QZ,Ding, CM,Zhang, XY,Zhang, LL,Wang, WF,Ali, S.

[10]Transcriptome-metabolome analysis reveals how sires affect meat quality in hybrid sheep populations. Bowen Chen,Yaojing Yue,Jianye Li,Jianbin Liu,Chao Yuan,Tingting Guo,Dan Zhang,Bohui Yang,Zengkui Lu. 2022

[11]Genetic Diversity and Population Structure of Fusarium commune Causing Strawberry Root Rot in Southcentral China. He, Yunlu,Chen, Jia,Tang, Chao,Deng, Qiao,Guo, Litao,Cheng, Yi,Li, Zhimin,Wang, Tuhong,Xu, Jianping,Gao, Chunsheng. 2022

[12]RNA-Seq Analysis Reveals Expression Regulatory Divergence of W-Linked Genes between Two Contrasting Chicken Breeds. Hongchang Gu,Liang Wang,Xueze Lv,Weifang Yang,Yu Chen,Kaiyang Li,Jianwei Zhang,Yaxiong Jia,Zhonghua Ning,Lujiang Qu. 2022

[13]Hybridization promotes growth performance by altering rumen microbiota and metabolites in sheep. Rui Zhang,Liwa Zhang,Xuejiao An,Jianye Li,Chune Niu,Jinxia Zhang,Zhiguang Geng,Tao Xu,Bohui Yang,Zhenfei Xu,Yaojing Yue. 2024

[14]Genomic signals of divergence and hybridization between a wild grape (Vitis adenoclada) and domesticated grape ('Shine Muscat'). Wenjing Peng,Feicui Liang,Zhuyifu Chen,Zhihong Gong,Mengyan Zhang,Rongfu Wei,Hongyan Li,Tianhao Zhang,Fengping Pan,Xiping Yang,Yongfeng Zhou,Zhiyao Ma,Xiaodong Xu,Daidong Wu. 2024

[15]Haplotype-resolved genomes of octoploid species in Phyllanthaceae family reveal a critical role for polyploidization and hybridization in speciation. Li, Fangping,Hou, Zhuangwei,Xu, Shiqiang,Han, Danlu,Li, Bin,Hu, Haifei,Liu, Jieying,Cai, Shike,Gan, Zhenpeng,Gu, Yan,Zhang, Xiufeng,Zhou, Xiaofan,Wang, Shaokui,Zhao, Junliang,Mei, Yu,Zhang, Jisen,Wang, Zefu,Wang, Jihua. 2024

[16]Phylogenomic insights into incomplete lineage sorting and hybridization in early-diverging eudicots. Yu Xin Liu,Xiao Mei Huang,Wen Chuang He,Andrey S. Erst,Kun Li Xiang,Wei Wang. 2025

[17]Genomic insights into the evolutionary history and conservation of the living fossil Tetracentron sinense. Jing, Zhao-Yang,Zhang, Ren-Gang,Liu, Yang,Cheng, Ke-Guang,Liu, De-Tuan,Shu, Heng,Kong, Jiali,Liu, Zhong-Hua,Ma, Yong-Peng,Liu, Ping-Li. 2025

[18]Changes in nutritional constituents, anthocyanins, and volatile compounds during the processing of black rice tea. Wu, Li,Zhai, Meijing,Yao, Yang,Ren, Guixing,Zhai, Meijing,Dong, Chuan,Shuang, Shaomin,Wu, Li. 2013

[19]Anthocyanin-rich Aronia melanocarpa extract improves body temperature maintenance in healthy women with a cold constitution. Aoi, Wataru,Sonoda, Keisuke,Iwata, Tomoaki,Li, Yanmei. 2013

[20]Anthocyanin Accumulation in Various Organs of a Teinturier Cultivar (Vitis vinifera L.) during the Growing Season. Guan, Le,Li, Ji-Hu,Fan, Pei-Ge,Wu, Ben-Hong,Guan, Le,Li, Ji-Hu,Chen, Sha,Li, Shao-Hua,Fang, Jin-Bao. 2012

作者其他论文 更多>>