数字农科院2.0

Single-cell transcriptional profiling reveals developmental dynamics of longissimus dorsi muscle in adult and Juvenile bactrian camels

文献类型： 外文期刊

作者： Baojun De;Yiyi Liu;Lu Li;Fanhua Meng;Yuwen Liu;Ting Jia;Yuting Chen;Chunxia Liu;Shenyuan Wang;Tao Li;Hongmei Xiao;Fang Wan;Yingchun Liu;Wenlong Wang;Huanmin Zhou;Wenguang Zhang;Xin Wen;Jie Gong;A. Naer;Hongmei Bao;Qian Song;Ha Si Chaolu;Hai Long;Yanru Zhang;Junwei Cao

作者机构：

关键词： bactrian camel;fibro-adipogenic progenitors;muscle fiber type;single-cell RNA sequencing;skeletal muscle development

期刊名称： Frontiers in Physiology

ISSN：

年卷期： 2025 年 16 卷

页码：

收录情况： SCIE（2025版）

摘要： Introduction: The longissimus dorsi muscle of Bactrian camels holds significant biological and economic value. However, the cellular heterogeneity, lineage differentiation patterns, and intercellular communication mechanisms underlying its skeletal muscle development remain unclear, which has hampered the advancement of precise regulation of camel meat quality traits and genetic improvement of the breed. Accordingly, there is an urgent need to elucidate the developmental regulatory mechanisms of this muscle tissue at the single-cell level. Methods: In this study, longissimus dorsi muscle tissues from 4-day-old (juvenile) and 5-year-old (adult) Bactrian camels were selected as research subjects. Integrated single-nucleus RNA sequencing (snRNA-seq) was employed to obtain gene expression data, which was coupled with Monocle2 pseudotime analysis, CellChat-based intercellular communication dissection, Gene Ontology (GO) enrichment analysis, and C2C12 cell functional validation experiments to conduct a systematic investigation into the cellular characteristics and developmental mechanisms of the Bactrian camel longissimus dorsi muscle. Results: A total of 14 cell clusters were identified, and the cellular composition of muscle tissues differed significantly between age groups—juvenile camel muscles were enriched with proliferative cell populations such as muscle satellite cells (MuSCs) and fibroblast-like progenitor cells (FAPs), while adult camel muscles were dominated by mature type IIX/IIA fast-twitch muscle fibers. Further analysis revealed that MuSCs exhibited bidirectional differentiation potential towards type I slow-twitch muscle fibers and type IIA/IIX fast-twitch muscle fibers, and the PCDH7 gene was found to promote myogenic differentiation. Additionally, four FAP subpopulations were characterized, among which the MME+ FAP subpopulation was closely associated with intramyocellular fat (IMF) deposition. Discussion: This study, for the first time, constructed a single-cell atlas and intercellular communication network of the Bactrian camel longissimus dorsi muscle, uncovered the key regulatory mechanisms governing its skeletal muscle development, and identified functionally important regulatory targets such as PCDH7. These findings not only provide a theoretical basis for the precise improvement of camel meat quality but also laid the groundwork for in-depth investigations into the adaptive evolutionary mechanisms of camel skeletal muscle.

分类号：

  • 相关文献

[1]Myofiber characteristics and eating quality of three major muscles from Chinese Simmental cattle. Lang, Yu-Miao,Wang, Yong-Feng,Sun, Bao-Zhong,Zhang, Song-Shan,Xie, Peng,Li, Hai-Peng,Sha, Kun,Zhang, Li.

[2]Myofiber Characteristics And Eating Quality O.f Three Major Muscles F rom Chinese Simmental Cattle. Lang, YM, Wang, YF, Sun, BZ, Zhang, SS, Xie, P, Sha, K, Zhang, L, Li, HP. 2017

[3]Characterization of RNA Editing in Oxidative and Glycolytic Skeletal Muscles of Yak. Shi, Yilin,Wu, Xuemei,Liang, Chunnian,Guo, Xian,Ma, Xiaoming,Yan, Ping,Chu, Min,Wu, Xiaoyun. 2026

[4]High occurrence of length heteroplasmy in domestic Bactrian camel (Camelus bactrianus). He, Xiaohong,Chen, Xiaofei,Pu, Yabin,Song, Shen,Dong, Kunzhe,Zhao, Qianjun,Guan, Weijun,Ma, Yuehui,Jiang, Lin,He, Xiaohong,Chen, Xiaofei,Pu, Yabin,Song, Shen,Han, Jianlin,Dong, Kunzhe,Zhao, Qianjun,Guan, Weijun,Ma, Yuehui,Jiang, Lin,Zhang, Wenbin,Han, Jianlin.

[5]Studies of "emaciation ailment" in the Bactrian camel. Shen, Xiaoyun,Li, Xia. 2010

[6]Anaplasma infection of Bactrian camels (Camelus bactrianus) and ticks in Xinjiang, China. Li, Youquan,Yang, Jifei,Chen, Ze,Qin, Gege,Li, Yaqiong,Li, Qian,Liu, Junlong,Liu, Zhijie,Guan, Guiquan,Yin, Hong,Luo, Jianxun,Zhang, Lin,Yin, Hong. 2015

[7]Bactrian camel (Camelus bactrianus) integrins alpha v beta 3 and alpha v beta 6 as FMDV receptors: Molecular cloning, sequence analysis and comparison with other species. Du, Junzheng,Gao, Shandian,Chang, Huiyun,Cong, Guozheng,Lin, Tong,Shao, Junjun,Liu, Zaixin,Liu, Xiangtao,Cai, Xuepeng. 2009

[8]Establishment of Bactrian Camel Induced Pluripotent Stem Cells and Prediction of Their Unique Pluripotency Genes. Zongshuai Li,Yina Li,Qiran Zhang,Wenbo Ge,Yong Zhang,Xingxu Zhao,Junjie Hu,Ligang Yuan,Wangdong Zhang. 2023

[9]High Occurrence Of Length Heteroplasmy I.n Domestic Bactrian Camel ( Camelus Bactrianus). He, XH, Chen, XF, Zhang, WB, Pu, YB, Song, S, Han, JL, Dong, KZ, Zhao, QJ, Guan, WJ, Ma, YH, Jiang, L. 2017

[10]Landscape of Global Gene Expression Reveals Distinctive Tissue Characteristics in Bactrian Camels (Camelus bactrianus). Yuanyuan Luan,Yan Fang,Lin Jiang,Yuehui Ma,Shangjie Wu,Junwen Zhou,Yabin Pu,Qianjun Zhao,Xiaohong He. 2022

[11]Molecular characterization of Cryptosporidium spp. in Bactrian camels (Camelus bactrianus) from Yili Kazak Autonomous Prefecture of Xinjiang, China. Rongsheng Mi,Amanguli Silayi,Yongsheng Wang,Chenyang Xia,Wenqiang Tang,Haiyan Gong,Yan Huang,Yan Zhang,Genqiang Yan,Zhaoguo Chen. 2024

[12]Whole-Genome Resequencing Analysis of the Camelus bactrianus (Bactrian Camel) Genome Identifies Mutations and Genes Affecting Milk Production Traits. Huaibing Yao,Zhangyuan Pan,Wanpeng Ma,Zhongkai Zhao,Zhanqiang Su,Jie Yang. 2024

[13]Characterization of MUSTN1 gene and its relationship with skeletal muscle development at postnatal stages in Pekin ducks. Xu, T. S.,Gu, L. H.,Liu, X. L.,Xu, T. S.,Xu, T. S.,Hou, S. S.,Zhang, X. H.,Sun, Y.,Ye, B. G.. 2015

[14]SMAD7, an antagonist of TGF-beta signaling, is a candidate of prenatal skeletal muscle development and weaning weight in pigs. Hua, Chaoju,Wang, Zishuai,Peng, Xing,Hou, Xinhua,Yang, Yalan,Li, Kui,Tang, Zhonglin,Li, Kui,Tang, Zhonglin,Zhang, Jianbing.

[15]Dynamic Expression of MicroRNA-127 During Porcine Prenatal and Postnatal Skeletal Muscle Development. Yang Ya-lan,Li Yan,Liang Ru-yi,Zhou Rong,Ao Hong,Mu Yu-lian,Yang Shu-lin,Li Kui,Tang Zhong-lin,Li Yan. 2014

[16]Comprehensive analysis of lncRNAs involved in skeletal muscle development in ZBED6-knockout Bama pigs. Dandan Wang,Yabin Pu,Yefang Li,Dengke Pan,Shengnan Wang,Wenjie Tian,Yuehui Ma,Lin Jiang. 2021

[17]Effects of Equol Supplementation on Growth Performance, Redox Status, Intestinal Health and Skeletal Muscle Development of Weanling Piglets with Intrauterine Growth Retardation. Yong Zhang,Jingchang Ren,Li Chen,Honglin Yan,Tiande Zou,Hongfu Zhang,Jingbo Liu. 2023

[18]Multiomics analysis reveals signatures of selection and loci associated with complex traits in pigs. Liu, Lei,Yi, Guoqiang,Yao, Yilong,Liu, Yuwen,Li, Jiang,Yang, Yalan,Liu, Mei,Fang, Lingzhao,Mo, Delin,Zhang, Longchao,Liu, Yonggang,Niu, Yongchao,Wang, Liyuan,Qu, Xiaolu,Pan, Zhangyuan,Wang, Lei,Chen, Muya,Fan, Xinhao,Chen, Yun,Zhang, Yongsheng,Li, Xingzheng,Wang, Zhen,Tang, Yijie,Huang, Hetian,Yuan, Pengxiang,Liao, Yuying,Li, Xinjian,Yin, Zongjun,Liu, Di,Zhang, Dongjie,Zhou, Quanyong,Wu, Wangjun,Jiang, Jicai,Gao, Yahui,Liu, George E.,Wang, Lixian,Chen, Yaosheng,Li, Kui,Groenen, Martien A. M.,Tang, Zhonglin. 2024

[19]Multiomics analysis reveals signatures of selection and loci associated with complex traits in pigs. Liu, Lei,Yi, Guoqiang,Yao, Yilong,Liu, Yuwen,Li, Jiang,Yang, Yalan,Liu, Mei,Fang, Lingzhao,Mo, Delin,Zhang, Longchao,Liu, Yonggang,Niu, Yongchao,Wang, Liyuan,Qu, Xiaolu,Pan, Zhangyuan,Wang, Lei,Chen, Muya,Fan, Xinhao,Chen, Yun,Zhang, Yongsheng,Li, Xingzheng,Wang, Zhen,Tang, Yijie,Huang, Hetian,Yuan, Pengxiang,Liao, Yuying,Li, Xinjian,Yin, Zongjun,Liu, Di,Zhang, Dongjie,Zhou, Quanyong,Wu, Wangjun,Jiang, Jicai,Gao, Yahui,Liu, George E.,Wang, Lixian,Chen, Yaosheng,Li, Kui,Groenen, Martien A. M.,Tang, Zhonglin. 2025

[20]The toggle switch model for gene expression change during duck skeletal muscle development. Dingbang Ding,Lionel Kinkpe,Yang Li,Yunsheng Zhang,Xia Wang. 2025

作者其他论文 更多>>