数字农科院2.0

Effects of coating on recovery of Escherichia coli-derived phytase under different steam pelleting conditions

文献类型： 外文期刊

作者： Wang, Yuming;Zhang, Hu;Cao, Ning;Qi, Bingqian;Zhao, Feng;Xie, Jingjing

作者机构：

关键词： coating;phytase;pig;recovery rate of activity;steam conditioning-pelleting

期刊名称： TRANSLATIONAL ANIMAL SCIENCE

ISSN：

年卷期： 2025 年 9 卷

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收录情况： ESCI（2025版）

摘要： The objective of this study was to evaluate the effect of coating on the recovery rate (RR) of phytase activity during the steam conditioning-pelleting (SCP) process. A split-plot design was employed, with phytases assigned to the main plot and two conditioning temperatures (75 or 85 degrees C) assigned to the subplot. The whole plot was repeated four times. In Exp. 1, six phytases were analyzed, including an uncoated phytase (NP) NP1 (NP1), two coated phytases (CP) CP1 and CP2 derived from NP1, and three commercial phytases (MP) MP1-MP3. In Exp. 2, coating technology was refined based on the results of Exp. 1, and nine phytases were analyzed, including an NP2, five coated phytases CP3-CP7 derived from NP2, and three commercial phytases MP4-MP6. Phytase activity after the steam-conditioning, pelleting, and cooling process was analyzed, and the RR of phytase activity was calculated for each process. In Exp. 1, significant interactions between phytase and conditioning temperature on the RR of phytase activity were observed (P < 0.05). The RR of CP1 and CP2 did not differ from that of NP1. Commercial phytase MP3 exhibited a lower RR than the other four phytases when conditioned at 75 degrees C (P < 0.05). Except for MP3, the RR of phytases decreased as the conditioning temperature increased (P < 0.05). In Exp. 2, the RR of phytase decreased as the conditioning temperature increased from 75 to 85 degrees C (P < 0.05). Compared with NP2, the RR increased, and the loss rate of activity for all five coated phytase (CP3-CP7) decreased after the conditioning process (P < 0.05). Commercial phytase MP4 and MP6 had comparable RR to NP2, while MP5 exhibited a comparable RR to CP3-CP7. In conclusion, the coating technology used in Exp. 1 did not increase the RR of phytase during the pelleting process, whereas the improved coating process employed in Exp. 2 effectively increased the thermostability of phytase. Lay Summary Feed processing often involves high temperatures that can reduce the effectiveness of enzymes like phytase, which helps animals digest phosphorus. This study explored how coating technologies can enhance the stability of phytase during the steam conditioning-pelleting (SCP) process, a common method used in feed production. Two experiments were conducted using different phytase products, including coated and uncoated types. In the first experiment, coating techniques did not improve the phytase thermostability during SCP process. However, by refining the coating process in the second experiment, researchers found that coated phytases maintained higher activity levels compared to uncoated ones. The enhanced coating technology reduced the loss rate of phytase activity under heat and moisture, proving its effectiveness in preserving phytase during feed production. These findings highlight the importance of developing advanced protective coatings for enzymes to improve their stability and functionality. This could lead to more efficient feed production and better nutrient utilization for livestock, ultimately benefiting both farmers and the environment.

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