数字农科院2.0

Characterizing the impact of CPSF30 gene disruption on TuMV infection in Arabidopsis thaliana

文献类型： 外文期刊

作者： Yanping Wei;Quan Yuan;Dalal Sulaiman Alshaya;Abdul Waheed;Kotb A. Attia;Sajid Fiaz;Muhammad Shahid Iqbal

作者机构：

关键词： （1-1-1）Antiviral strategies;Arabidopsis;CPSF30;gene expression;mRNA-seq;TuMV

期刊名称： GM Crops and Food

ISSN： 2164-5698

年卷期： 2024 年 15 卷 1 期

页码：

收录情况： SCIE（2024版）

摘要： CPSF30, a key polyadenylation factor, also serves as an m6A reader, playing a crucial role in determining RNA fate post-transcription. While its homologs mammals are known to be vital for viral replication and immune evasion, the full scope of CPSF30 in plant, particular in viral regulation, remains less explored. Our study demonstrates that CPSF30 significantly facilitates the infection of turnip mosaic virus (TuMV) in Arabidopsis thaliana, as evidenced by infection experiments on the engineered cpsf30 mutant. Among the two isoforms, CPSF30-L, which were characterized with m6A binding activity, emerged as the primary isoform responding to TuMV infection. Analysis of m6A components revealed potential involvement of the m6A machinery in regulating TuMV infection. In contrast, CPSF30-S exhibited distinct subcellular localization, coalescing with P-body markers (AtDCP1 and AtDCP2) in cytoplasmic granules, suggesting divergent regulatory mechanisms between the isoforms. Furthermore, comprehensive mRNA-Seq and miRNA-Seq analysis of Col-0 and cpsf30 mutants revealed global transcriptional reprogramming, highlighting CPSF30’s role in selectively modulating gene expression during TuMV infection. In conclusion, this research underscores CPSF30’s critical role in the TuMV lifecycle and sets the stage for further exploration of its function in plant viral regulation.

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