数字农科院2.0

Improving pre-harvest sprouting resistance in rice by editing OsABA8ox using CRISPR/Cas9

文献类型： 外文期刊

作者： Fu K.; Song W.; Chen C.; Mou C.; Huang Y.; Zhang F.; Hao Q.; Wang P.; Ma T.; Chen Y.; Zhu Z.; Zhang M.; Tong Q.; Liu X.; Jiang L.; Wan J.

作者机构：

关键词： abscisic acid;mixed function oxidase;phytohormone;plant protein;CRISPR Cas system;dormancy;gene expression regulation;genetics;germination;metabolism;Oryza;plant seed;Abscisic Acid;CRISPR-Cas Systems;Gene Expression Regulation, Plant;Germination;Mixed Function Oxygenases;Oryza;Plant Dormancy;Plant Growth Regulators;Plant Proteins;Seeds

期刊名称： Plant Cell Reports

ISSN： 0721-7714

年卷期： 2022 年 41.0 卷 10.0 期

页码：

收录情况： SCIE（2022版）

摘要： Key message: Knock out OsABA8ox helps improve pre-harvest spouting resistance and do not affect rice yield. Pre-harvest sprouting(PHS) is a phenomenon that the seeds of crops germinate preharvest, which reduces the yield and quality of rice. Abscisic acid(ABA) is one of the phytohormones that promotes seed dormancy. ABA8’ hydroxylase is the main enzyme that can catabolism ABA in plant. There are three genes that encode ABA8’ hydroxylase in rice, named OsABA8ox1, OsABA8ox2 and OsABA8ox3. In this study, we use CRISPR/Cas9 gene editing technology to target these three genes in Ningjing6 and find that the knockout transgenic lines are all significantly strengthen in seed dormancy and have no effect on the yield. By a series of quantitative experiments, we consider that after knock out OsABA8ox, the high endogenous ABA level will influence the ABA signal which suppress the substantial and energy metabolism in the seeds, and finally led to higher dormancy. © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

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