数字农科院2.0

Quantitative phosphoproteomic analysis of chicken DF-1 cellsinfected with Eimeria tenella, using tandem mass tag (TMT) andparallel reaction monitoring (PRM) mass spectrometry

文献类型： 外文期刊

作者： Liu Shu Jia;Zhan Liu;Shun Hai Zhu;Qi Ping Zhao;Hong Yu Han;Huan Zhi Zhao;Yu Yu;Hui Dong

作者机构：

关键词： Coccidiosis;DF-1 cells;Eimeira tenella;Phosphoproteomics;TMT

期刊名称： Parasite (Paris, France)

ISSN： 1776-1042

年卷期： 2024 年 31 卷

页码：

收录情况： SCIE（2024版）

摘要： Eimeria tenella is an obligate intracellular parasite which causes great harm to the poultry breeding industry. Protein phosphorylation plays a vital role in host cell-E. tenella interactions. However, no comprehensive phosphoproteomic analyses of host cells at various phases of E. tenella infection have been published. In this study, quantitative phosphoproteomic analysis of chicken embryo DF-1 fibroblasts that were uninfected (UI) or infected with E. tenella for 6 h (PI6, the early invasion phase) or 36 h (PI36, the trophozoite development phase) was conducted. A total of 10,122 phosphopeptides matched to 3,398 host cell phosphoproteins were identified and 13,437 phosphorylation sites were identified. Of these, 491, 1,253, and 275 differentially expressed phosphorylated proteins were identified in the PI6/UI, PI36/UI, and PI36/PI6 comparisons, respectively. KEGG pathway enrichment analysis showed that E. tenella modulated host cell processes through phosphorylation, including focal adhesion, regulation of the actin cytoskeleton, and FoxO signaling to support its early invasion phase, and modulating adherens junctions and the ErbB signaling pathway to favor its trophozoite development. These results enrich the data on the interaction between E. tenella and host cells and facilitate a better understanding of the molecular mechanisms underlying host-parasite relationships.

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