文献类型： 外文期刊

作者： Wu, Zihui;Sun, Nan;Tang, Jiabin;Xu, Linghua;Zhou, Xinrui;Jiang, Menglei;Cheng, Jiarui;Zhu, Xuedan;Liu, Miaoyu;Yang, Liguo;Davis, John S.;Hua, Guohua

作者机构：

关键词： （1-1-5）SH;glycoform;follicular development;pre-antral follicles;antral follicles;ovulation

期刊名称： BIOLOGY OF REPRODUCTION

ISSN： 0006-3363

年卷期： 2025 年

页码：

收录情况： SCIE（2025版）

摘要： Follicle-stimulating hormone is the primary gonadotropin regulating ovarian follicular development and is widely utilized in assisted reproduction. Our previous research indicated that different glycoforms of the follicle-stimulating hormone Beta-subunit influence follicular development in vivo, but their specific roles across various folliculogenesis stages remained unclear. In this study, we compared two commercial follicle-stimulating hormone preparations: pituitary-derived follicle-stimulating hormone, which contains both fully and hypo- glycosylated Beta-subunit forms, and recombination follicle-stimulating hormone, predominantly consisting of fully glycosylated forms. Using an in vitro follicular culture model, we observed that pituitary-derived follicle-stimulating hormone was more effective than recombination follicle-stimulating hormone in stimulating follicular growth and promoting antrum formation in preantral follicles. Pituitary-derived follicle-stimulating hormone also rescued insufficient preantral follicle growth and antrum formation caused by recombination follicle-stimulating hormone. Besides, pituitary-derived follicle-stimulating hormone more efficiently drove small antral follicle growth than recombination follicle-stimulating hormone. Additionally, pituitary-derived follicle-stimulating hormone significantly increased the granulosa cells proliferation and effectively inhibited apoptosis, thereby protecting growing follicles from atresia. Pituitary-derived follicle-stimulating hormone also induced higher steroidogenesis than recombination follicle-stimulating hormone. Mechanistically, pituitary-derived follicle-stimulating hormone activated key downstream signaling including protein kinase A/cAMP-response element binding protein, mitogen-activated protein kinase/extracellular signal-regulated kinase, and phosphatidylinositol 3-kinase/AKT more efficiently than recombination follicle-stimulating hormone. Importantly, pre-treating antral follicles with pituitary-derived follicle-stimulating hormone significantly improved ovulation rate following human chorionic gonadotropin administration. This was linked to a rapid upregulation of luteinizing hormone/choriogonadotropin receptor expression and enhanced mitogen-activated protein kinase/extracellular signal-regulated kinase signaling activity. Collectively, our data support that pituitary-derived follicle-stimulating hormone, with its mixture of fully and hypo-glycosylated forms, has greater bioactivity in promoting preantral-antral transition, antral follicle growth, and ovulation compared to recombination follicle-stimulating hormone, which mainly consists of fully glycosylated follicle-stimulating hormone. Application of mixed glycoforms or hypo-glycosylated follicle-stimulating hormone may improve the outcomes in assisted reproduction. Sentence: Pituitary-derived FSH, a mixture of fully and hypo-glycosylated forms, has greater bioactivity in facilitating murine follicular development and ovulation in vitro compared to recombinant FSH which primarily contains fully glycosylated FSH.

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