数字农科院2.0

A colorimetric/fluorescent dual-mode aptasensor for the rapid and sensitive detection of Listeria monocytogenes

文献类型： 外文期刊

作者： Runze Zhang;Xiaoxu Wang;Pan Hu;Yuzhu Wang;Kai Jiang;Ruoran Shi;Yongjie Yang

作者机构：

关键词： chemistry;food science;sensor

期刊名称： iScience

ISSN： 2589-0042

年卷期： 2025 年 28 卷 9 期

页码：

收录情况： SCIE（2025版）

摘要： Listeria monocytogenes, a major foodborne pathogen, causes a serious infection. Therefore, better detection methods are needed to prevent outbreaks. Herein, a colorimetric/fluorescent dual-mode aptasensor using (GlcN)5, AuNPs, and QD-COOH was developed for its rapid detection. The probe was an L. monocytogenes-specific aptamer with cDNA attached to carboxyl magnetic beads (Apt-cDNA-CMBs). In the presence of L. monocytogenes, the aptamer binds to it, making cDNA detach and adsorb onto AuNPs, inhibiting (GlcN)5-induced aggregation and keeping the AuNPs solution red. Meanwhile, dispersed AuNPs quench QDs, leading to reduced fluorescence. Without L. monocytogenes, AuNPs aggregate, turning the solution blue, and QD fluorescence remains unchanged. The lowest detection limits (LODs) were 4.21 CFU/mL (colorimetry) and 8.89 CFU/mL (fluorescence). The aptasensor showed excellent recoveries in beef (85.11%–107.53%) and salmon (86.21%–109.98%). This dual-mode aptasensor offered a promising solution for simple, rapid, and reliable on-site detection of L. monocytogenes.

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