数字农科院2.0

Extraction, structural characterization, and in vitro immunomodulatory activities of Saccharomyces cerevisiae spore wall β-glucan

文献类型： 外文期刊

作者： Mengqing Yan;Xue Wang;Jingtao Zhang;Shuheng Yang;Cong Pan;Shiwei Liu;Jia Liu;Youqiang Yu;Fangli Ding;Shenglin Duan;Huoqing Huang;Guoyu Liu;Peng Yuan

作者机构：

关键词： Immune mechanism;Saccharomyces cerevisiae spore;Structural analysis;β-Glucan

期刊名称： Journal of Agriculture and Food Research

ISSN： 2666-1543

年卷期： 2025 年 22 卷

页码：

收录情况： ESCI（2025版）

摘要： Saccharomyces cerevisiae spores exposing β-glucan layers exhibit notable immunomodulatory properties, suggesting their potential as a natural and efficient source of β-glucans. However, studies on the application of spore wall β-glucans are limited, and their precise structural characteristics and immunological mechanisms remain poorly understood. This study aimed to investigate the structural characteristics and immunomodulatory properties of β-glucan extracted from the yeast spores (YSG) and compare them with those of β-glucan derived from vegetative cells (YG). Similar to YG, YSG consists of a backbone chain of β-(1,3) glucan and side chains of β-(1,6) glucan with a natural triplex structure. However, there are differences in the branching ratio and helical structure between YSG and YG. YSG has fewer branches and a less pronounced triple helix structure than YG. The particle size (222.4 nm) and molecular weight (Mw: 1392.27 kDa) of YSG were found to be significantly smaller than those of YG. Additionally, YSG can bind to the Dectin-1 receptor protein on the surface of macrophages, activating the Syk/CARD9/NF-κB pathway to promote the expression of iNOS and cytokine mRNAs, thereby facilitating the release of NO and related cytokines for increased immunostimulatory effects. Collectively, our results provide foundational insights into the potential of yeast spore wall β-glucan as a functional immunostimulatory agent.

分类号：

  • 相关文献

[1]Multi-Architecture Nanovaccines Against Pseudorabies Virus and Particle Size-Governed Immune Activation Mechanisms. Meng Zhang,ChunxinWang,Cailing Meng,Xinglong Xu,Jinghui Zhan,Ying Li,QingWang,Junqian Pan,Haixin Cui,Xiang Zhao. 2025

[2]Role of peach proteins in juice precipitation induced by high pressure CO2. Zhao, Feng,Zhou, Linyan,Wang, Yongtao,Liao, Xiaojun,Zhou, Linyan. 2016

[3]Genome-wide identification of auxin response factor (ARF) genes and its tissue-specific prominent expression in Gossypium raimondii. Runrun Sun,Kunbo Wang,Tenglong Guo,Don C. Jones,Juliana Cobb,Baohong Zhang,Qinglian Wang.

[4]Systematic analysis and expression of Gossypium 2ODD superfamily highlight the roles of GhLDOXs responding to alkali and other abiotic stress in cotton. Tiantian Jiang,Aihua Cui,Yupeng Cui,Ruifeng Cui,Mingge Han,Yuexin Zhang,Yapeng Fan,Hui Huang,Xixian Feng,Yuqian Lei,Xiaoyu Liu,Kesong Ni,Hong Zhang,Nan Xu,Jing Wang,Liangqing Sun,Cun Rui,Junjuan Wang,Shuai Wang,Xiugui Chen,Xuke Lu,Delong Wang,Lixue Guo,Lanjie Zhao,Fushun Hao,Wuwei Ye. 2023

[5]Acidic polysaccharide from corn silk: Structural & conformational properties and hepatoprotective activity. Yi Zhang,Lianmou Yao,Yanfang Liu,Bingjie Chen,Chunfang Wang,Kuijie Gong,Feng Wang,Yongjin Qiao. 2023

[6]Structural analysis and immunological activity of a novel low molecular weight neutral polysaccharide isolated from Hemerocallis citrina Borani. Yongrui Ti,Yanli Zhang,Yuqing Hou,Yuqian Ban,Xiaoxiao Wang,Guoliang Li,Zihan Song. 2025

[7]Using Fourier transform near infrared spectroscopy to estimate the nutritional value in whole and milled naked oats. Liu, Hao,Ren, Guixing,Zhou, Haitao.

[8]Complexation of soybean protein isolate with β-glucan and myricetin: Different affinity on 7S and 11S globulin by QCM-D and molecular simulation analysis. Lei D.,Li J.,Zhang C.,Li S.,Zhu Z.,Wang F.,Deng Q.,Grimi N.. 2022

[9]The effect and underlying mechanism of yeast β-glucan on antiviral resistance of zebrafish against spring viremia of carp virus infection. Hui Liang,Yu Li,Ming Li,Wei Zhou,Jie Chen,Zhen Zhang,Yalin Yang,Chao Ran,Zhigang Zhou. 2022

[10]Influence of ultrasound assisted metal-free Fenton reaction on the structural characteristic and immunostimulatory activity of a β-D-glucan isolated from Dictyophora indusiata. Wu D.-T.,Zhao Y.-X.,Yuan Q.,Wang S.,Gan R.-Y.,Hu Y.-C.,Zou L.. 2022

[11]Expression and characterization of a novel microbial GH9 glucanase, IDSGLUC9-4, isolated from sheep rumen. Yongzhen Zhu,Shuning Bai,Nuo Li,Jun Hong Wang,Jia Kun Wang,Qian Wang,Kaiying Wang,Tietao Zhang. 2024

[12]Improvement in Storage Stability and Physicochemical Properties of Whole-Grain Highland Barley Pulp Prepared by a Novel Industry-Scale Microfluidizer System in Comparison with Colloid Milling. Hong Zhu,Wenjie Xu,Tianyu Zhang,Tao Jin,Bing Fang,Ju Qiu. 2024

[13]Effect of dynamic high pressure microfluidization on pasting, gelling and rheological properties of starch composite with β-glucan both from highland barley. Ju Qiu,Wenjie Xu,Tianyu Zhang,Bing Fang,Yongqiang Cheng,Hong Zhu. 2025

[14]Effect of β-glucans with different molecular weights on pasting, rheological, texture properties, and in vitro digestibility of normal and waxy highland barley starches. Xueqing Wang,Ruoqi Sun,Xuebing Wang,Xiangping Zhang,Shuoya Zeng,Jingjing Yang,Lang Jia,Jingfeng Li,Bei Fan,Jia Liu,Yatao Huang,Fengzhong Wang,Lili Wang. 2025

作者其他论文 更多>>