数字农科院2.0

Identification of Rice Leaf Width Gene FLW11 Through Genome-Wide Association Study and Functional Analysis

文献类型： 外文期刊

作者： Yang Yulu;Zhang Yanfang;Liu Xiong;Zhang Lihua;Huang Jingfen;Shen Lixing;Zhao Huibo;Shen Lan;Zhang Qiang;Zhu Li;Hu Jiang;Ren Deyong;Gao Zhenyu;Dong Guojun;Qiao Weihua;Qian Qian;Zhang Guangheng

关键词： rice;leaf width;genome-wide association analysis;QTL;haplotype

期刊名称： Rice Science

ISSN： 1672-6308

年卷期： 2025 年

页码：

收录情况： SCIE（2025版） ; ; CSCD（2025-2026年度） ; ; 科技核心（2024版） ; ; 农林核心（2024版）

摘要： The width of rice leaves determines the size of photosynthetic area. Optimizing rice leaf width can improve the photosynthetic rate, thereby increasing rice yield. In the past few years, several leaf width genes have been identified, however few of them could be used in rice production due to limited natural variation. In this study, 225 rice germplasm resources were used conducting genome-wide association analysis (GWAS) to explore the genetic basis of rice flag leaf width. We identified 9 QTLs associated with leaf width, with phenotypic contribution rates ranging from 3.17% to 14.37%. A chromosome fragment replacement line (NPBY476) with enlarged leaf width was constructed from Nipponbare (NPB) and wild rice (O. rufipogon, Y476), and lots of near-isogenic lines were developed from this line for fine mapping of qFLW11. We narrowed the qFLW11 in 87Kb interval, which contains five genes. Knock out mutant of LOC_Os11g41530 exhibited wider leaf, suggesting it might be the candidate gene FLW11. Furthermore, LOC_Os11g41530 can be divided into three major haplotypes, among which Hap2 could enhance the rice leaf width, thereby improving single plant yield. Our results identified a novel leaf width gene FLW11, which provides avaluable genetic resource for leaf shape design in modern rice breeding.

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