数字农科院2.0

A VLP-Based Vaccine Displaying HBHA and MTP Antigens of Mycobacterium tuberculosis Induces Protective Immune Responses in M. tuberculosis H37Ra Infected Mice

文献类型： 外文期刊

作者： Juan Wang;Tao Xie;Inayat Ullah;Youjun Mi;Xiaoping Li;Yang Gong;Pu He;Yuqi Liu;Fei Li;Jixi Li;Zengjun Lu;Bingdong Zhu

作者机构：

关键词： Heparin-binding hemagglutinin;Mycobacterium tuberculosis;Mycobacterium tuberculosis pili;subunit vaccine;virus-like particles

期刊名称： Vaccines

ISSN： 2076-393X

年卷期： 2023 年 11 卷 5 期

页码：

收录情况： SCIE（2023版）

摘要： Heparin-binding hemagglutinin (HBHA) and M. tuberculosis pili (MTP) are important antigens on the surface of Mycobacterium tuberculosis. To display these antigens effectively, the fusion protein HBHA-MTP with a molecular weight of 20 kD (L20) was inserted into the receptor-binding hemagglutinin (HA) fragment of influenza virus and was expressed along with matrix protein M1 in Sf9 insect cells to generate influenza virus-like particles (LV20 in short). The results showed that the insertion of L20 into the envelope of the influenza virus did not affect the self-assembly and morphology of LV20 VLPs. The expression of L20 was successfully verified by transmission electron microscopy. Importantly, it did not interfere with the immunogenicity reactivity of LV20 VLPs. We demonstrated that LV20 combined with the adjuvant composed of DDA and Poly I: C (DP) elicited significantly higher antigen-specific antibodies and CD4+/CD8+ T cell responses than PBS and BCG vaccination in mice. It suggests that the insect cell expression system is an excellent protein production system, and LV20 VLPs could be a novel tuberculosis vaccine candidate for further evaluation.

分类号：

  • 相关文献

[1]The combination of Mycobacterium tuberculosis fusion proteins LT33 and LT28 induced strong protective immunity in mice. Pu He,Juan Wang,Daquan Tan,Lina Hu,Yanlin Ma,Youjun Mi,Fei Li,Tingting Zhang,Yunjie Du,Wenhua Zhang,Jixi Li,Lei Jiao,Bingdong Zhu. 2024

[2]Characterization of Rv0394c gene encoding hyaluronidase and chondrosulfatase from Mycobacterium tuberculosis. Yuan, Xianglian,Chen, Liping,Cao, Jun,Yu, Shenye,Liu, Siguo,Yuan, Xianglian,Deng, Xiaoxia,Pang, Hai,Wang Quankai. 2013

[3]Development of one-tube multiplex polymerase chain reaction (PCR) for detecting Mycobacterium bovis. Quan, Zhang,Haiming, Tan,Xiaoya, Cai,Weifeng, Yuan,Hong, Jia,Hongfei, Zhu.

[4]Crystallization and preliminary X-ray analysis of a novel esterase Rv0045c from Mycobacterium tuberculosis. Xu, Lipeng,Liu, Siguo,Xu, Lipeng,Guo, Jiubiao,Zheng, Xiangdong,Pang, Hai,Wen, Tingyi,Sun, Fei. 2010

[5]Expression, Purification and Characterisation of Secreted Esterase Rv2525c from Mycobacterium tuberculosis. Dang, Guanghui,Chen, Liping,Li, Zhaoli,Cui, Yingying,Cao, Jun,Yu, Shenye,Liu, Siguo,Deng, Xiaoxia,Pang, Hai.

[6]C-terminal heat shock protein 70 of Mycobacterium tuberculosis as a molecular adjuvant for DNA vaccination with the porcine circovirus type 2 ORF2 (capsid) gene in mice. Fu, Fang,Tian, Huabin,Li, Xuesong,Lang, Yuekun,Liu, Siguo,Li, Haizhong,Wang, Wei,Li, Xi,Chen, Xin,Li, Xuesong,Tong, Guangzhi. 2013

[7]A novel B cell epitope in cold-shock DEAD-box protein A from Mycobacterium tuberculosis. Wang, Huanan,Zhu, Ting,Yu, Shenye,Liu, Huifang,Wang, Xiumei,Chen, Liping,Si, Wei,Liu, Siguo,Wang, Huanan,Pang, Hai.

[8]Characterization of a novel exported esterase Rv3036c from Mycobacterium tuberculosis. Chen, Liping,Dang, Guanghui,Cao, Jun,Yu, Shenye,Liu, Siguo,Dang, Guanghui,Deng, Xiaoxia,Pang, Hai,Dang, Guanghui,Wu, Defeng.

[9]Characterization Of A Novel Mycobacterium Tuberculosis Serine Protease (Rv3194C) Activity And Pathogenicity. Li, H, Dang, GH, Liu, HX, Wang, ZX, Cui, ZY, Song, NN, Chen, LP, Liu, SG. 2019

[10]Extracellular Sphingomyelinase Rv0888 Of Mycobacterium T.uberculosis Contributes To Pathological L ung Injury Of Mycobacterium Smegmatis In Mice Via Inducing Formation Of Neutrophil Extracellular Traps. Dang, GH, Cui, YY, Wang, L, Li, TT, Cui, ZY, Song, NN, Chen, LP, Pang, H, Liu, SG. 2018

[11]Pe17 Protein From Mycobacterium Tuberculosis E.nhances Mycobacterium Smegmatis Survival I n Macrophages And Pathogenicity In Mice. Li, Z, Liu, HX, Li, HF, Dang, GH, Cui, ZY, Song, NN, Wang, QK, Liu, SG, Chen, LP. 2019

[12]PE12 interaction with TLR4 promotes intracellular survival of Mycobacterium tuberculosis by suppressing inflammatory response. Jiajun Zhang,Yingying Cui,Xinxin Zang,Tingting Feng,Fanruo Chen,Hui Wang,Guanghui Dang,Siguo Liu. 2023

[13]Plasma-based ultrasensitive detection of Mycobacterium tuberculosis ESAT6/CFP10 fusion antigen using a CRISPR-driven aptamer fluorescence testing (CRAFT). Siyuan Liu,Guohui Xiao,Peixuan Li,Yating Xu,Xinhao Fan,Lin Tian,Su Zhang,Guoliang Zhang. 2025

[14]Deep Amplicon Sequencing Reveals Culture-dependent Clonal Selection of Mycobacterium tuberculosis in Clinical Samples. Jiuxin Qu,Wanfei Liu,Shuyan Chen,Chi Wu,Wenjie Lai,Rui Qin,Feidi Ye,Yuanchun Li,Liang Fu,Guofang Deng,Lei Liu,Qiang Lin,Peng Cui. 2025

[15]Development and application of a curcumin-cinnamon essential oil nanoemulsion agent against mycobacteria. Lei, Zhihui,Ren, Yixuan,Wang, Jinyao,Shi, Haisu,Lin, Hu,Chen, Hong,Bi, Lijun,Wang, Yutang,Zhang, Hongtai. 2025

[16]Cross-sectional analysis of tuberculosis burden and risk factors in Swabi district, Khyber Pakhtunkhwa, Pakistan. Iqbal Ahmad,Madiha Fatima,Ilyas Khan,Sulaiman Bahadar,Abdul Qadeer,Mohamad S. Hakim. 2025

[17]Generation and characterization of a new mammalian cell line continuously expressing virus-like particles of Japanese encephalitis virus for a subunit vaccine candidate. Hua, Rong-Hong,Li, Ye-Nan,Chen, Zhen-Shi,Liu, Li-Ke,Huo, Hong,Wang, Xiao-Lei,Guo, Li-Ping,Shen, Nan,Wang, Jing-Fei,Bu, Zhi-Gao. 2014

[18]Identification of novel Haemophilus parasuis serovar 5 vaccine candidates using an immunoproteomic approach. Li, Gang,Xie, Fang,Li, Jianjun,Liu, Jiao,Li, Dapeng,Zhang, Yanhe,Liu, Siguo,Wang, Chunlai,Langford, Paul R.,Li, Yanwen.

[19]Immune response in cattle inoculated with the recombinant complete polyprotein of foot-and-mouth disease virus from Bombyx mori larvae. Li ZhiYong,Yi YongZhu,Yin XiangPing,Zhang ZhiFang,Liu JiXing.

[20]Recombinant gp90 protein expressed in Pichia pastoris induces a protective immune response against reticuloendotheliosis virus in chickens. Li, Kai,Gao, Honglei,Gao, Li,Qi, Xiaole,Gao, Yulong,Qin, Liting,Wang, Yongqiang,Wang, Xiaomei. 2012

作者其他论文 更多>>