数字农科院2.0

Identification of gene OsZIPH1 related to rice shattering using Bulk Segregant Analysis

文献类型： 外文期刊

作者： Bing Han;Zichao Zhu;Xiaoding Ma;Ying Xiong;Di Cui;Chutao Wang;Li Chen;Xianyong Li;Longzhi Han

作者机构：

关键词： Abscission layer;Bulk Segregant Analysis;F2:3 segregating population;Gene knockout;Rice shattering

期刊名称： Current Plant Biology

ISSN： 2214-6628

年卷期： 2025 年 44 卷

页码：

收录情况： ESCI（2025版）

摘要： Grain shattering is one of the critical traits in rice, influencing harvesting methods and yield. Seeds from varieties that shatter easily often begin to drop before reaching full maturity. Varieties with difficult-to-thresh or non-threshing characteristics are susceptible to having their branches broken and mixed with straw during mechanical harvesting, leading to relatively severe losses. Therefore, developing rice varieties with a moderate degree of shattering, suitable for production applications, is the primary focus in rice breeding. In this study, the F2:3 generation population from a cross between the easy-shattering weedy rice variety "H21" and the non-shattering japonica rice variety "Longdao 18" was utilized to investigate shattering resistance through phenotypic identification, Bulk Segregant Analysis (BSA), gene editing, and cytological detection. Gene Os01g0935000, a ZOS1–23 - C2H2 zinc finger protein involved in the regulation of shattering, was preliminarily localized and named OsZIPH1 . Mutant phenotype identification showed that the shattering rate of the mutant is 41.98 %, while that of the wild type is 84.33 %, the decrease is 50.2 % compared to the wild type H21. Cytological analysis revealed that mutant spikelets had incomplete abscission layer structures between the lemma and rachis branches, whereas wild-type plants exhibited clearly defined abscission layer structures. The expression level of this gene in the wild type is 2.63 times that in the knockout mutant. Haplotype analysis has revealed that this gene comprises five haplotypes. HAP5 is unique to Oryza ruf ipogon, while HAP1 is found in nearly all japoni ca rice varieties. HAP2 is predominantly present in 70 % of indica weedy rice, and HAP3 is primarily associated with 59.37 % of indica improved rice. HAP4 is found in SH and BHA weedy rice, indicating that the shattering gene Os01g 0935000 plays a significant role in the domestication process of rice. These findings enhance our understanding of the biological mechanisms underlying rice shattering and are crucial for developing rice varieties with desirable shattering characteristics.

分类号：

  • 相关文献

[1]Mechanism of pod shattering in the forage legume Medicago ruthenica. Mao W. Guo,Lin Zhu,Hong Y. Li,Wan P. Liu,Zi N. Wu,Cheng H. Wang,Lei Liu,Zhi Y. Li,Jun Li. 2022

[2]Advances in Rice Seed Shattering. Hao Wu,Qi He,Quan Wang. 2023

[3]Genetic Dissection Of The Fuzzless S.eed Trait In Gossypium B arbadense. Du, Xiongming,Zhu, Qian-Hao,Yuan, Yuman,Zhu, Qian-Hao,Wilson, Iain,Stiller, Warwick,Wang, Pengpeng,Jia, Yinhua,Pan, Zhaoe,Llewellyn, Danny. 2018

[4]Linkage mapping of a dominant male sterility gene Ms-cd1 in Brassica oleracea. Wang, XW,Lou, P,Bonnema, G,Yang, BJ,He, HJ,Zhang, YG,Fang, ZY. 2005

[5]Genetic mapping and molecular characterization of the delayed green gene dg in watermelon (Citrullus lanatus). Haileslassie Gebremeskel,Muhammad Jawad Umer,Zhu Hongju,Bingbing Li,Zhao Shengjie,Pingli Yuan,Lu Xuqiang,He Nan,Liu Wenge. 2023

[6]Whole Genome Resequencing From Bulked P.opulations As A Rapid Q tl And Gene Identification Method In Rice. Zegeye, WA, Zhang, YX, Cao, LY, Cheng, SH. 2018

[7]Integrated genomic and transcriptomic analysis reveals genes associated with plant height of foxtail millet. Zhu, Mengyuan,He, Qiang,Lyu, Mingjie,Shi, Tiantian,Gao, Qian,Zhi, Hui,王欢,Jia, Guanqing,Tang, Sha,Cheng, Xiliu,Wang, Rui,Xu, Andi,Wang, Haigang,Qiao, Zhijun,Liu, Jun,Diao, Xianmin,Gao, Ying. 2023

[8]Identification of genomic regions and candidate genes underlying carotenoid accumulation in soybean using next-generation sequen-cing based bulk segregant analysis. Berhane S. Gebregziabher,Shengrui Zhang,Jing Li,Bin Li,Junming Sun. 2025

[9]Thioredoxin reductase from Toxoplasma gondii: an essential virulence effector with antioxidant function. Xue, Junxin,Jiang, Wei,Chen, Yongjun,Wang, Minyan,Wang, Quan,Xue, Junxin,Gong, Fengju,Zeng, Peng,Xia, Can,Huang, Kehe,Xue, Junxin.

[10]Multiprotein-bridging factor 1 regulates vegetative growth, osmotic stress, and virulence in Magnaporthe oryzae. Huang, Hao,Zhang, Heng,Zhao, Ao,Chen, Libin,Chen, Ruiqi,Wang, Zonghua,Lu, Guo-dong,Zhang, Kai,Li, Guangpu.

[11]Genome-Wide CRISPR/Cas9 Screen Identifies New Genes Critical for Defense Against Oxidant Stress in Toxoplasma gondii. Yun Chen,Qi Liu,Jun Xin Xue,Man Yu Zhang,Xiao Ling Geng,Quan Wang,Wei Jiang. 2021

[12]Carbamoyl phosphate synthase subunit cgcps1 is necessary for virulence and to regulate stress tolerance in colletotrichum gloeosporioides. Aamar Mushtaq,Muhammad Tariq,Maqsood Ahmed,Zongshan Zhou,Imran Ali,Raja Tahir Mahmood. 2021

[13]Advance of Clustered Regularly Interspaced Short Palindromic Repeats-Cas9 System and Its Application in Crop Improvement. Rao, Yuchun,Yang, Xi,Pan, Chenyang,Wang, Chun,Wang, Kejian. 2022

[14]Tandem Mass Tags Quantitative Proteome Identification and Function Analysis of ABC Transporters in Neofusicoccum parvum. Chen, Jie,Han, Shan,Li, Shujiang,Zhu, Hanmingyue,Li, Shuying,Yan, Junjie,Zhu, Tianhui. 2022

[15]Genome-Wide Identification and Characterization of Homeobox Transcription Factors in Phoma sorghina var. saccharum Causing Sugarcane Twisted Leaf Disease. Yixue Bao,Jinlan Deng,Sehrish Akbar,Zhenzhen Duan,Chi Zhang,Wenfeng Lin,Suyan Wu,Yabing Yue,Wei Yao,Jianlong Xu,Muqing Zhang. 2024

[16]Highly efficient gene editing of Feline herpesvirus 1 using CRISPR/Cas9 combined with FACS. Hai Ming Wang,Shi Jia Xu,Bing Yan Cai,Wen Ying Qiu,Hui Lu,Yan Dong Tang. 2025

[17]Identification and Evolution of Salt-Tolerant Genes in Weedy Rice Through High-Throughput Resequencing. Bing Zou,Bing Han,Jianchang Sun,Mingmao Sun,Xiaoding Ma,Li Chen,Di Cui,Jing Ma,Xiaohong Guo,Longzhi Han. 2025

作者其他论文 更多>>