数字农科院2.0

Silkworm (Bombyx mori) gut bacteria respond positively to exposure of magnetic Fe3O4@urease-doped nanoconjugates: potential reconstruction for nutritional efficiency

文献类型： 外文期刊

作者： Herman, Richard Ansah;Khurshid, Marriam;Zhang, Zhan-Peng;Yan, Cheng-Hai;Ayepa, Ellen;Li, Yi-Jiang-Cheng;You, Shuai;Sheng, Sheng;Wang, Jun

作者机构：

关键词： Magnetic Fe3O4@urease nanoconjugates;Silkworm (Bombyx mori);Gut bacteria;Feed efficiency;Protein metabolism

期刊名称： ANIMAL MICROBIOME

ISSN：

年卷期： 2025 年 8 卷 1 期

页码：

收录情况： ESCI（2025版）

摘要： BackgroundThe gut microbiota of the silkworm (Bombyx mori) plays a vital role in metabolic processes, including nutrient digestion and immune regulation. However, the specific bacterial communities that enhance feed utilization and improve amino acid composition in silk and pupae remain unclear. In this study, we explored the mechanisms through which gut bacteria influence nutritional efficiency and the biosynthesis of high-quality silk and pupae by exposing fifth-instar silkworms to magnetic Fe3O4@urease-doped nanoconjugates. Results Our findings revealed that Fe3O4@urease nanoconjugates had no detrimental effects on the silkworm gut. Dominant genera in the MF group included Enterococcus, Staphylococcus, Pantoea, Klebsiella and Glutamicibacter (p < 0.05), while Linear Discriminant Analysis (LDA) and Effect Size (LEfSe) identified Dialister, Rhizobium and Parabacteroides as biomarkers. Functional prediction revealed bacteria in the Fe3O4@urease nanoconjugates treatment group were involved in metabolic activities, including amino acid synthesis, protein degradation and immune development. HPLC-LC-MS analysis revealed significantly higher levels of the amino acids Histidine (1077.06%) and Arginine (1020.45%) in the Fe3O4@urease nanoconjugates treatment group. Conclusion In summary, magnetic Fe3O4@urease-doped nanoconjugates effectively modulated B. mori gut microbiota, enhancing digestion and nutrient assimilation, and providing a scientific basis for the rational implementation of bacteria control for enhancing amino acid contents in silk and pupae.

分类号：

  • 相关文献

[1]Proteomic-Based Approach To The Proteins I.nvolved In 1-Deoxynojirimycin Accumulation I n Silkworm Bombyx Mori (Lepidoptera: Bombycidae). Pan, Gang,Olatunji, Opeyemi J.,Liu, Yueqin,Chen, Hongxia,Ouyang, Zhen,Chen, Hongxia,Wang, Wenbing. 2018

[2]Altering fibroin heavy chain gene of silkworm Bombyx mori by homologous recombination. Zhao, Y,Chen, X,Peng, WP,Dong, L,Huang, JT,Lu, CD. 2001

[3]The effects of heat stress on protein metabolism in lactating Holstein cows. Gao, S. T.,Guo, J.,Quan, S. Y.,Nan, X. M.,Fernandez, M. V. Sanz,Baumgard, L. H.,Bu, D. P.,Bu, D. P..

[4]Joint Transcriptome and Metabolome Analysis Prevails the Biological Mechanisms Underlying the Pro-Survival Fight in In Vitro Heat-Stressed Granulosa Cells. Abdul Sammad,Hanpeng Luo,Lirong Hu,Shanjiang Zhao,Jianfei Gong,Saqib Umer,Adnan Khan,Huabin Zhu,Yachun Wang. 2022

[5]Maternal supplementation with mulberry-leaf flavonoids improves the development of skeletal muscle in the offspring of chickens. Zhenwu Huang,Hongjian Dai,Simeng Li,Zhe Wang,Quanwei Wei,Zhonghua Ning,Yuming Guo,Fangxiong Shi,Zengpeng Lv. 2024

[6]Effects of dietary scFOS and lactobacilli on survival, growth, and disease resistance of hybrid tilapia. Liu, Wenshu,Wang, Wenwen,Ran, Chao,He, Suxu,Yang, Yaling,Zhou, Zhigang,Liu, Wenshu.

[7]Pollen Reverses Decreased Lifespan, Altered N.utritional Metabolism And Suppressed I mmunity In Honey Bees (Apis Mellifera) Treated With Antibiotics. Li, JH, Heerman, MC, Evans, JD, Rose, R, Li, WF, Rodriguez-Garcia, C, DeGrandi-Hoffman, G, Zhao, YZ, Huang, SK, Li, ZG, Hamilton, M, Chen, YP. 2019

[8]High-fiber diet and rope-skipping benefit cardiometabolic health and modulate gut microbiota in young adults: A randomized controlled trial. Zongyu Lin,Xin Zhang,Miao Wu,Yingan Ming,Xiaotong Wang,Hailin Li,Fenglian Huang,Fei Gao,Yanna Zhu. 2023

[9]Effects of choline chloride on intestinal microbiota and its association with liver fat accumulation in zebrafish. Wenshu Liu,Xiaoze Guo,Yanliang Chen,Yanqiang Tang,Haihong Xiao,Yuzhu Wang,Siming Li,Zhigang Zhou. 2022

[10]Identification of WxL and S-Layer Proteins from Lactobacillus brevis with the Ability to Bind Cellulose and Xylan. Zhenzhen Hao,Wenjing Zhang,Xiaolu Wang,Yuan Wang,Xing Qin,Huiying Luo,Huoqing Huang,Xiaoyun Su. 2022

[11]Gut symbiotic bacteria enhance reproduction in Spodoptera frugiperda (J.E. Smith) by regulating juvenile hormone III and 20-hydroxyecdysone pathways. Bo Chu,Shishuai Ge,Wei He,Xiaoting Sun,Jiajie Ma,Xianming Yang,Chunyang Lv,Pengjun Xu,Xincheng Zhao,Kongming Wu. 2025

[12]Native mid-gut bacterial community increases resistance to nucleopolyhedrovirus in the cotton leafworm. Tong Li,Yueli Jiang,Yunhui Zhang,Yuqing Wu,Kenneth Wilson,Pengjun Xu. 2025

[13]Gut bacteria are essential for development of an invasive bark beetle by regulating glucose transport. Fanghua Liu,Fangyuan Ye,Yunwen Yang,Zhiwei Kang,Yang Liu,Wei Chen,Saige Wang,Hongru Kou,Le Kang,Jianghua Sun. 2024

[14]Nonspecific Binding of a Putative S-Layer Protein to Plant Cell Wall Polysaccharides—Implication for Growth Competence of Lactobacillus brevis in the Gut Microbiota. Zhenzhen Hao,Wenjing Zhang,Jianzhong Ge,Daoxin Yang,Kairui Guo,Yuan Wang,Huiying Luo,Huoqing Huang,Xiaoyun Su. 2025

[15]Corn peptides alleviate high-fat diet-induced obesity and metabolic disorders in mice by regulating adipogenesis and intestinal microbial disorders. Feng, Zhiyuan,Yong, Wang、张瑞雪、Li, Mingliang,Pang, Meixia,Zhang, Xinru,Wei, Ying,Sun, Guiju. 2025

[16]The host phylogeny and climate determine the gut bacteria of global insects. Shiqing Nie,Yong Jun Liu,Yuan Ge. 2025

[17]Effects of dietary vitamin E on immunological stress of layers and their offspring. Zhao, G. P.,Han, M. J.,Zheng, M. Q.,Zhao, J. P.,Chen, J. L.,Wen, J..

[18]Combined effect of microbially derived cecal SCFA and host genetics on feed efficiency in broiler chickens. Zhengxiao He,Ranran Liu,Mengjie Wang,Qiao Wang,Jumei Zheng,Jiqiang Ding,Jie Wen,Alan G. Fahey,Guiping Zhao. 2023

[19]Dietary oregano essential oil supplementation improves intestinal functions and alters gut microbiota in late-phase laying hens. Jia Feng,Mingyuan Lu,Jing Wang,Haijun Zhang,Kai Qiu,Guanghai Qi,Shugeng Wu. 2021

[20]Variations in Microbial Diversity and Metabolite Profiles of Female Landrace Finishing Pigs With Distinct Feed Efficiency. Zhixin Wang,Yingzhi He,Chuduan Wang,Hong Ao,Zhen Tan,Kai Xing. 2021

作者其他论文 更多>>