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Evaluation of the Pathogenicity of Highly Virulent Eurasian Genotype II African Swine Fever Virus with MGF505-2R Gene Deletion in Piglets

文献类型： 外文期刊

作者： Xu, Fan;Huang, Huaguo;Dang, Wen;Du, Yu;Li, Tao;Liu, Huanan;Shi, Zhengwang;Tian, Hong;He, Jijun;Zheng, Haixue

作者机构：

关键词： African swine fever virus;MGF505-2R;live attenuated vaccine;pathogenicity;immune response

期刊名称： VIRUSES-BASEL

ISSN：

年卷期： 2025 年 17 卷 12 期

页码：

收录情况： SCIE（2025版）

摘要： African swine fever virus (ASFV) poses a significant threat to the global pig industry due to high mortality rates and complex genetic variation. Live attenuated vaccines (LAVs) provide protection against ASFV. Previously, MGF505-2R was identified as a potent inhibitor of innate immunity in vitro. This study evaluates the pathogenicity of a recombinant Eurasian genotype II strain with the MGF505-2R gene deleted (ASFV-Delta MGF505-2R) in piglets. Twelve five-week-old crossbred piglets were divided into two groups, with one group of eight piglets inoculated with ASFV-Delta MGF505-2R (n = 8) and the other group of four piglets inoculated with the same dose of parental ASFV CN/GS 2018 (n = 4). Clinical symptoms, viral loads, and immune responses were monitored over 30 days. ASFV-Delta MGF505-2R-inoculated piglets exhibited transient fever and low viremia only in the beginning of the challenge, while the control group developed high levels of viremia and hyperthermia at day 2 and 8 post-challenge, respectively. Meanwhile, the control group demonstrated more severe post-mortem signs and immuno-histochemistry injury when compared to the Delta MGF505-2R group. ELISA analysis displayed higher levels of IFN-beta and IL-1 beta in the Delta MGF505-2R group, further solidating the immunosuppressive role of MGF505-2R. All ASFV-Delta MGF505-2R-inoculated piglets developed high titers of ASFV-specific P30 antibodies at 10 days post-challenge. These findings rationalized the potential of ASFV-Delta MGF505-2R as a live attenuated candidate for ASF infection.

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