数字农科院2.0

Integrated transcriptomic and metabolomic analysis reveals mechanisms of eggshell color variation in Pekin ducks

文献类型： 外文期刊

作者： Longxin Wang;Simeng Yu;Yunsheng Zhang;Zhanbao Guo;Shuaiqin Wang;Shuisheng Hou

作者机构：

关键词： biliverdin;eggshell color;eggshell gland;liver;WGCNA

期刊名称： Poultry Science

ISSN： 0032-5791

年卷期： 2025 年 104 卷 11 期

页码：

收录情况： SCIE（2025版）

摘要： Green-shelled eggs are widely recognized for their high quality, nutritional value, and superior shell strength, making them highly attractive to consumers. In ducks, the trait for green shells is dominant, while the trait for white shells is recessive. Notably, even within homozygous dominant populations, considerable variation in eggshell color intensity remains, affecting product uniformity and market value. In this study, homozygous green-shelled laying ducks at 30 and 70 weeks of age into three groups based on shell color measurements: dark-green, medium-green, and light-green. The content of biliverdin decreased significantly with age and exhibited considerable variation among the three-color groups, while protoporphyrin IX content showed no consistent trend or significant differences. A highly significant positive correlation was found between the thickness of the shell gland epithelium and biliverdin content in in ducks at 30 weeks, which remained significant at 70 weeks. Furthermore, at 70 weeks, shell color correlated positively with biliverdin content in the shell gland, where both biliverdin and biliverdin IX beta levels were significantly higher than those found in the liver. Transcriptomic analysis revealed that ST13 gene expression decreased with age, concurrent with lightening of shell color. Metabolomic profiling showed that phosphatidylethanolamine (PE) and phosphatidylcholine (PC) contents in the shell gland increased with darker shell color and were significantly positively correlated with ST13 expression. Weighted Gene Co-expression Network Analysis (WGCNA) suggested that ST13 is co-expressed with ALAS1 and may be involved in regulating porphyrin metabolism, as well as the metabolic pathways of glycine, serine, and threonine.

分类号：

  • 相关文献

[1]Transcriptome-based analysis reveals relationship between duck eggshell color and eggshell strength. Longxin Wang,Hehe Liu,Simeng Yu,Meixi Lu,Yunsheng Zhang,Shuaiqin Wang,Shuisheng Hou. 2025

[2]中国美利奴羊胚胎骨骼肌发育的加权基因共表达网络分析. 石田培,侯浩宾,王欣悦,赵志达,尚明玉,张莉. 2020

[3]利用加权基因共表达网络分析筛选天农麻鸡胴体性状候选基因. 赵迪,康慧敏,谭晓冬,刘冉冉,张正芬,李华,赵桂苹. 2022

[4]基于WGCNA与GSEA方法挖掘调控中卫山羊羊毛弯曲相关基因. 李晓波,刘占发,刘悦,陈倩,马月辉,赵倩君,叶绍辉. 2022

[5]Eggshell depigmentation in the late phase of production is associated with altered Microbiota and Metabolism of the uterus in laying hens. Dong Dai,Libing Gao,Yingli Pan,Chaojiang Chen,Kaixuan Ma,Haijun Zhang,Shugeng Wu,Guanghai Qi,Jing Wang. 2025

[6]Effects of dietary supplementation with copper sulfate or tribasic copper chloride on broiler performance, relative copper bioavailability, and oxidation stability of vitamin E in feed. Luo, XG,Ji, F,Lin, YX,Steward, FA,Lu, L,Liu, B,Yu, SX. 2005

[7]Chronic Heat Stress Induces Immune Response, Oxidative Stress Response, and Apoptosis of Finishing Pig Liver: A Proteomic Approach. Cui, Yanjun,Hao, Yue,Li, Jielei,Li, Gan,Gao, Yanli,Gu, Xianhong,Bao, Weiguang. 2016

[8]Cholesterol metabolism changes in the early stages of ascites in broiler chickens. Zhang, Shan,Zhao, Xu,Shen, Yiru,Shi, S. R.,Tong, H. B.,Guo, Y. M.,Shi, S. R..

[9]Changes in liver triglyceride metabolism in broiler chickens with cold-induced ascites syndrome. Zhao, Xu,Zhang, Shan,Shen, Yiru,Shi, S. R.,Guo, Y. M.,Shi, S. R..

[10]GROWTH PERFORMANCE AND ANTIOXIDANT CAPACITIES IN PIGLETS WITH DIFFERENT WEANING AGE. Li, L. A.,Yang, J. J.,Li, Y.,Jin, T. M.,Li, R. N.,Qin, S. Y.,Xie, J. J.,Du, G. M.. 2016

[11]Expression analyses and antiviral properties of the Beijing-You and White Leghorn myxovirus resistance gene with different amino acids at position 631. Du, L. X.,Zhao, G. P.,Zhang, C. S..

[12]Effects of aluminum chloride on serum proteins, bilirubin, and hepatic trace elements in chickens. Wang, Ben,Zhang, Hongling,Liu, Liming,Li, Guojiang,Song, Yongli,Zhu, Yanzhu,Li, Yanfei.

[13]Oral MSG administration alters hepatic expression of genes for lipid and nitrogen metabolism in suckling piglets. Chen, Gang,Zhang, Jun,Zhang, Yuzhe,Liao, Peng,Li, Tiejun,Yin, Yulong,Chen, Gang,Chen, Lixiang,Wang, Jinquan,Wu, Guoyao,Wu, Guoyao.

[14]CCAAT-enhancer binding protein (C/EBP) beta regulates insulin-like growth factor (IGF) 1 expression in porcine liver during prenatal and postnatal development. Tang, Yiting,Xiong, Kai,Shen, Ming,Liu, Honglin,Tang, Yiting,Mu, Yulian,Li, Kui,Xiong, Kai.

[15]Copper bioavailability, blood parameters, and nutrient balance in mink. Wu, X. Z.,Guo, J. G.,Liu, Z.,Gao, X. H.,Wu, X. Z.,Zhang, T. T.,Yang, F. H.,Yang, F. H..

[16]cDNA Microarray on Differentially Expressed Genes of Liver Tissue Between Bulls and Steers of Simmental. Wang, Shuhui,Lian, Zhengxing,Wang, Shuhui,Zhou, Zhengkui,Huai, Yahong,Ji, Aiguo,Gao, Xue,Li, Junya,Chen, Jinbao,Xu, Shangzhong,Wang, Shuxin. 2011

[17]Unraveling molecular mechanistic differences in liver metabolism between lean and fat lines of Pekin duck (Anas platyrhynchos domestica): A proteomic study. Zheng, Aijuan,Chang, Wenhuan,Zhang, Shu,Cai, Huiyi,Chen, Guilan,Lou, Ruiying,Liu, Guohua,Hou, Shuisheng.

[18]Probiotic (Enterococcus faecium) induced responses of the hepatic proteome improves metabolic efficiency of broiler chickens (Gallus gallus). Zheng, Aijuan,Luo, Jianjie,Meng, Kun,Chang, Wenhuan,Zhang, Shu,Liu, Guohua,Yao, Bin,Li, Jianke,Bryden, Wayne L.,Wang, L. X. N.. 2016

[19]Proteomic analysis of liver development of lean Pekin duck (Anas platyrhynchos domestica). Zhang, Yunsheng,Hou, Shuisheng,Zheng, Aijuan,Liu, Guohua,Chang, Wenhuan,Zhang, Shu,Cai, Huiyi,Chen, Guilan.

[20]Evaluation of bioaccumulation and toxic effects of copper on hepatocellular structure in mice. Wang, Xuezhi,Wang, Hui,Li, Jianxi,Yang, Zhiqiang,Zhang, Jingyan,Qin, Zhe,Wang, Lei,Kong, Xiaojun.

作者其他论文 更多>>