数字农科院2.0

A Microsurgical Technique for Removing the Spermatheca of Bumblebee Females and Its Application

文献类型： 外文期刊

作者： Mingsheng Zhuang;Fan Yang;Zhongyan Xia;Yu Fei;Fugang Liu;Zhengyi Zhang;Zhihao Zhang;Jilian Li

作者机构：

关键词： a microsurgical technique;Bombus terrestris;bumblebee;social insect;spermatheca

期刊名称： Insects

ISSN： 2075-4450

年卷期： 2025 年 16 卷 7 期

页码：

收录情况： SCIE（2025版）

摘要： To solve the technical bottleneck caused by the absence of a feasible method for removing the spermatheca in social insects, we developed a microsurgical technique specifically designed for bumblebee females. In this study, the invention of this technique is based on the anatomical characteristics of the sting chamber of bumblebees and uses a bespoke scalpel to precisely remove the spermatheca, which is small in size and deeply embedded within the body. During the removal operation, a small wound was observed and a small amount of hemolymph flowed out. The wound healed very quickly and the survival rate of treated individuals was high. The results showed that there was no significant impact on the critical life activities of queens and workers, including longevity, mating behavior, oviposition capacity, and overwintering survival rate after the spermatheca was removed using this technique. These findings further confirm the feasibility and applicability of the technique and provide strong technical support for exploring the evolutionary dynamics and potential function of the spermatheca in social insects.

分类号：

  • 相关文献

[1]The factors affecting the success of the artificial insemination of bumblebees and technical improvements. Mingsheng Zhuang,Jilian Li. 2025

[2]Identification of reference genes for gene expression analysis at different developmental stages of the bumblebee Bombus terrestris (Hymenoptera: Apidae). Haoran Qin,Kun Dong,Jiaxing Huang,Shaoyu He,Jie Wu. 2021

[3]Untargeted Lipidomics Analysis Unravels the Different Metabolites in the Fat Body of Mated Bumblebee (Bombus terrestris) Queens. Yueqin Guo,Fugang Liu,Yulong Guo,Yingping Qu,Zhengyi Zhang,Jun Yao,Jin Xu,Jilian Li. 2023

[4]熊蜂为温室茄子授粉试验. 安建东,童越敏,国占宝,彭文君,吴杰,孙永深,李乃光. 2004

[5]The use of drone congregation behaviour for population surveys of the honey bee Apis cerana. Thomas Hagan,Julianne Lim,Gabriele Buchmann,Guiling Ding,Benjamin P. Oldroyd,Rosalyn Gloag. 2024

[6]A new gene, SRP16, differentially expressed in the spermathecae of honeybee queens (Apis mellifera) related with reproduction status. Wuxiang, Danping,Pan, Gang,Wu, Liming,Li, Jilian,Zheng, Huoqing.

[7]IRP30 promotes worker egg-laying in bumblebee, Bombus terrestris (Hymenoptera: Apidae). Yakai Tian,Jiyao Liu,Feng He,Jiaxing Huang,Jie Wu,Shaoyu He. 2021

[8]Worker-born males are smaller but have similar reproduction ability to queen-born males in bumblebees. Huiyue Zhao,Yanjie Liu,Hong Zhang,Tom D. Breeze,Jiandong An. 2021

[9]Gut microbiota is a potential factor in shaping phenotypic variation in larvae and adults of female bumble bees. Guo B.,Tang J.,Ding G.,Mashilingi S.K.,Huang J.,An J.. 2023

[10]Myzus persicae management through combined use of beneficial insects and thiacloprid in pepper seedlings. Qingcai Lin,Hao Chen,Xiaoyan Dai,Shuyan Yin,Chenghao Shi,Zhenjuan Yin,Jinping Zhang,Feng Zhang,Li Zheng,Yifan Zhai. 2021

[11]Characterization and Developmental Expression Patterns of Four Hexamerin Genes in the Bumble Bee, Bombus terrestris (Hymenoptera: Apidae). Yakai Tian,Yingping Qu,Kun Dong,Shaoyu He,Wu Jie,Jiaxing Huang. 2021

[12]Effects of Low-Temperature Acclimation on Nutrients of Bumble Bee Bombus terrestris Queens during Prediapause and Diapause. Mengnan Shi,Kun Dong,Jie Wu,Jiaxing Huang. 2023

[13]Imidacloprid reduces the mating success of males in bumblebees. Chen X.,Wang Y.,Zhou Y.,Wang F.,Wang J.,Yao X.,Imran M.,Luo S.. 2024

[14]Insights into adult worker foraging dynamics within a Bombus terrestris (Hymenoptera: Apidae) colony. Wang, Feiran,Dai, Jiangrui,Xie, Lixing,Chen, Xing,Guo, Shengnan,Wang, Jian,Yao, Xudong,Imran, Muhammad,Li-Byarlay, Hongmei,Luo, Shudong. 2024

[15]Comparative blueberry pollination efficacy of Bombus terrestris and two Apis species in greenhouses. Ji, Wei,Fan, Ying,Huang, Jiaxing,Xu, Jin,Li, Xiaoying,Yang, Huipeng,Guo, Yueqin,Wu, Yueguo,Li, Jilian,Yao, Jun. 2025

[16]Molecular characterization of a phospholipid-hydroperoxide glutathione peroxidase from the bumblebee Bombus ignitus. Hu, Zhigang,Lee, Kwang Sik,Choo, Young Moo,Sohn, Hung Dae,Jin, Byung Rae,Yoon, Hyung Joo,Kim, Iksoo,Wei, Ya Dong,Gui, Zhong Zheng,Zhang, Guo Zheng.

[17]Molecular cloning and oxidative stress response of a sigma-class glutathione S-transferase of the bumblebee Bombus ignitus. Kim, Bo Yeon,Hui, Wei Li,Lee, Kwang Sik,Wan, Hu,Jin, Byung Rae,Hui, Wei Li,Chen, Shan,Yoon, Hyung Joo,Gui, Zhong Zheng.

[18]Sensitivities of three bumblebee species to four pesticides applied commonly in greenhouses in China. Wu, Jie,Hu, Fu-Liang,Wu, Jie,Li, Ji-Lian,Peng, Wen-Jun. 2010

[19]Diversity of nosema associated with bumblebees (Bombus spp.) from China. Schmid-Hempel, Paul,Schmid-Hempel, Regula,Li, Jilian,Wu, Jie,Peng, Wenjun,An, Jiandong,Chen, Wenfeng.

[20]Transcript expression bias of phosphatidylethanolamine binding protein gene in bumblebee, Bombus lantschouensis (Hymenoptera: Apidae). Dong, Jie,Han, Lei,Wang, Ye,Huang, Jiaxing,Wu, Jie.

作者其他论文 更多>>