数字农科院2.0

Sublethal aflatoxin B1 exposure triggers multidimensional damage in honeybee (Apis mellifera) midgut: Integrative evidence from histomorphology, transcriptomics, and metagenomics

文献类型： 外文期刊

作者： Xuanpeng Liu;Jilian Li;Chuan Ma

作者机构：

关键词： Gut microbiota;Histopathology;Immune response;Mycotoxin;Oxidative stress

期刊名称： Journal of Hazardous Materials

ISSN： 0304-3894

年卷期： 2026 年 503 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Aflatoxin B1 (AFB1), a highly carcinogenic mycotoxin produced by Aspergillus fungi, has been increasingly identified as a global contaminant in bee pollen. Chronic exposure of honeybees (Apis mellifera) to AFB1-contaminated pollen poses substantial threats to colony health, yet its toxicological impacts remain poorly characterized despite the critical ecological role of these pollinators. In this study, we employed a multidimensional approach to investigate the toxicological effects of sublethal AFB1 exposure on honeybee midgut by integrated morphological, transcriptomic, and metagenomic analyses. Histopathological examination revealed severe midgut epithelium damage, including nuclear disintegration and enhanced apoptosis. Transcriptomic profiling coupled with enzyme activity assays unveiled significant dysregulation in immune response and oxidative stress-related pathways. Furthermore, metagenomic sequencing indicated substantial midgut microbiota alterations, characterized by a pronounced reduction in microbial diversity and beneficial microbe levels. These findings elucidate sublethal AFB1-induced honeybee health deterioration at cellular, molecular, and microbial levels, advancing our understanding of mycotoxin impacts on pollinators.

分类号：

  • 相关文献

[1]Unrevealing the therapeutic potential of artesunate against emerging zoonotic Babesia microti infection in the murine model. Saqib Ali Fazilani,Wei An,Sihong Li,Mohammad Farooque Hassan,Muhammad Ishfaq,Shakeel Ahmed Lakho,Muhammad Farooque,Muhammad Shoaib,Xiuying Zhang. 2024

[2]Dynamics of oxidative stress and immune responses in neonatal calves during diarrhea. Fu Z.L.,Yang Y.,Ma L.,Malmuthuge N.,Guan L.L.,Bu D.P.. 2024

[3]Effects of DHAV-3 infection on innate immunity, antioxidant capacity, and lipid metabolism in ducks with different DHAV-3 susceptibilities. Cao J.,Wen Z.,Zhang Y.,Zhang B.,Chen Y.,Xing G.,Wu Y.,Zhou Z.,Liu X.,Hou S.. 2024

[4]Abundance of Lactobacillus in porcine gut microbiota is closely related to immune response following PRRSV immunization. He Zhang,Wenjie Ma,Zhi Sun,Changkang Zhu,Gebremeskel Mamu Werid,Yassein M. Ibrahim,Wenli Zhang,Yu Pan,Dongfang Shi,Hongyan Chen,Yue Wang. 2021

[5]Abundance of Lactobacillus in porcine gut microbiota is closely related to immune response following PRRSV immunization. He Zhang,Wenjie Ma,Zhi Sun,Changkang Zhu,Gebremeskel Mamu Werid,Yassein M. Ibrahim,Wenli Zhang,Yu Pan,Dongfang Shi,Hongyan Chen,Yue Wang. 2021

[6]Colonization resistance: the role of gut microbiota in preventing Salmonella invasion and infection. Deng, Lei,Wang, Shaohui. 2024

[7]Pichia pastoris composition expressed aerolysin mutant of Aeromonas veronii as an oral vaccine evaluated in zebrafish (Danio rerio). Yao, Yuan-Yuan,Zhang, Qing-Shuang,Liu, Shu-Bin,Yang, Hong-Wei,Chen, Xing-Yu,Yang, Ya-Lin,Gao, Chen-Chen,Ran, Chao,Teame, Tsegay,Zhang, Zhen,Zhou, Zhi-Gang. 2024

[8]Hydroxytyrosol attenuates diquat-induced oxidative stress by activating Nrf2 pathway and modulating colonic microbiota in mice. Hui Han,Ruqing Zhong,Shunfen Zhang,Mengyu Wang,Xiaobin Wen,Bao Yi,Yong Zhao,Liang Chen,Hongfu Zhang. 2023

[9]Supplementation With Chinese Medicinal Plant Extracts From Lonicera hypoglauca and Scutellaria baicalensis Mitigates Colonic Inflammation by Regulating Oxidative Stress and Gut Microbiota in a Colitis Mouse Model. Fan Wan,Mengyu Wang,Ruqing Zhong,Liang Chen,Hui Han,Lei Liu,Yong Zhao,Huiyuan Lv,Fujiang Hou,Bao Yi,Hongfu Zhang. 2022

[10]Selenomethionine Ameliorates Cognitive Impairment, Decreases Hippocampal Oxidative Stress and Attenuates Dysbiosis in D-Galactose-Treated Mice. Ying Gao,Yongquan Xu,Junfeng Yin. 2022

[11]The Interaction between Oxidative Stress Biomarkers and Gut Microbiota in the Antioxidant Effects of Extracts from Sonchus brachyotus DC. in Oxazolone-Induced Intestinal Oxidative Stress in Adult Zebrafish. Yang J.,Zhou W.-W.,Shi D.-D.,Pan F.-F.,Sun W.-W.,Yang P.-L.,Li X.-M.. 2023

[12]Cereal polyphenols inhibition mechanisms on advanced glycation end products and regulation on type 2 diabetes. Dong, Lezhen,Li, Ying,Chen, Qin,Liu, Yahui,Wu, Zufang,Pan, Daodong,Yan, Ning,Liu, Lianliang. 2023

[13]Effects of tea against alcoholic fatty liver disease by modulating gut microbiota in chronic alcohol-exposed mice. Bangyan Li,Qianqian Mao,Dandan Zhou,Min Luo,Renyou Gan,Hangyu Li,Siyu Huang,Adila Saimaiti,Ao Shang,Huabin Li. 2021

[14]Hydroxytyrosol Alleviates Intestinal Oxidative Stress by Regulating Bile Acid Metabolism in a Piglet Model. Xiaobin Wen,Fan Wan,Ruqing Zhong,Liang Chen,Hongfu Zhang. 2024

[15]Sika Deer Velvet Antler Peptide Exerts Neuroprotective Effect in a Parkinson’s Disease Model via Regulating Oxidative Damage and Gut Microbiota. Ying Liu,Hongyuan Li,Min Yang,Jia Guo,Zepeng Sun,Shuyue Wang,Ru Li,Xin Pang,Yumi Kim,Xiaohui Wang,Yinghua Peng. 2024

[16]Network Analysis of Gut Microbial Communities Reveals Key Reason for Quercetin Protects against Colitis. Yanan Lv,Jing Peng,Xiaoyu Ma,Zeyi Liang,Ghasem Hosseini Salekdeh,Qunhua Ke,Wenxiang Shen,Zuoting Yan,Hongsheng Li,Shengyi Wang,Xuezhi Ding. 2024

[17]Gut microbiota dysbiosis and oxidative damage in high-fat diet-induced impairment of spermatogenesis: Role of protocatechuic acid intervention. Hu, Ruizhi,Yang, Xizi,Wang, Long,Su, Dingding,He, Ziyu,Li, Jiaxing,Gong, Jiatai,Zhang, Wentao,Ma, Siqi,Shi, Mingkun,Lv, Jing,Zhang, Qianjin,Hou, De-Xing,Zhang, Hongfu,He, Jianhua,Yin, Yulong,Wang, Jun,Wu, Shusong. 2024

[18]Lactiplantibacillus plantarum FRT4 protects against fatty liver hemorrhage syndrome: regulating gut microbiota and FoxO/TLR-4/NF-κB signaling pathway in laying hens. Daojie Li,Kun Meng,Guohua Liu,Zhiguo Wen,Yunsheng Han,Weiwei Liu,Xin Xu,Liye Song,Hongying Cai,Peilong Yang. 2025

[19]Mangosteen Pericarp Extract Mitigates Diquat-Induced Hepatic Oxidative Stress by NRF2/HO-1 Activation, Intestinal Barrier Integrity Restoration, and Gut Microbiota Modulation. Weichen Huang,Yujie Lv,Chenhao Zou,Chaoyue Ge,Shenao Zhan,Xinyu Shen,Lianchi Wu,Xiaoxu Wang,Hongmeng Yuan,Gang Lin,Dongyou Yu,Bing Liu. 2025

[20]Lactobacillus plantarum QL01 Alleviates D-Galactose-Induced Oxidative Stress and Restores Gut Microbiota in Ageing Mice. Li, Haichuan,Zhang, Mingqing,Wu, Diyan,Gong, Di,Huang, Jiazhang,Tang, Zhenchuang,Wang, Liang,Zhang, Ying. 2025

作者其他论文 更多>>