数字农科院2.0

Identification of core candidate genes responding to Verticillium wilt(Verticillium dahliae) in cotton via integrated methods

文献类型： 外文期刊

作者： 孙国清

关键词： 0

期刊名称： International Journal of Biological Macromolecules

ISSN： 0141-8130

年卷期： 2025 年

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Cotton is a vital natural fiber and oil crop, yet it is severely affected by verticillium wilt (VW), known as the ‘cancer’ of cotton, hindering the industry’s sustainable development. Upland cotton, which is widely cultivated, lacks effective resistance to VW, while most sea island cotton shows strong resistance. In this study, an F2:3 population was constructed by hybridizing the verticillium wilt-resistant island cotton variety ‘Hai7124’ with the susceptible variety ‘Xinhai14’. Using Bulked Segregant Analysis (BSA-seq), we identified 10 genetic intervals significantly associated with resistance. Additionally, two pathogenic strains of Verticillium dahliae, Vd592 (a strong pathogenic type) and VdKT (a weak pathogenic type), were used to infect the ‘Hai7124’ and ‘Xinhai14’ for RNA-seq analysis, focusing on differentially expressed genes and signaling pathways in samples treated with different resistant and susceptible materials and infected with different pathogens. By integrating BSA-seq and RNA-seq association analyses, the candidate gene range was further refined. Five genes (GBMYB102, GBWRKY65, GBRDA2, GBSOT16, and GBCWINV1) were validated through virus-induced gene silencing (VIGS). The results revealed that reduced expression of these genes significantly decreases plant disease resistance and leads to a reduction in the activity of defense-related enzymes (such as SOD, CAT or PAL) and secondary metabolites (including lignin or flavonoids). Based on the preliminary functional analysis of these candidate genes, we speculate that redox metabolism and secondary metabolites play crucial roles in the resistance of island cotton to Verticillium wilt, and that the resistance of island cotton to verticillium wilt is the result of multiple genes working together.

分类号：

  • 相关文献

[1]棉花抗黄萎病相关基因GhDIR1的生物学功能分析. 黄雨欣,, 谢涛 ,,王省芬,, 郭惠明 , 程红梅 2, 马伯军 1, 陈析丰 , 苏晓峰. 2025

[2]耐除草剂转基因大豆的创制与应用. 邱丽娟,林敏,郭勇. 2025

[3]Developing high‐value‐added cotton via rebuilding astaxanthin biosynthesis pathway. Liqing Miao 1,2,†, Yameng Xu 1,3,† , Xinyuan Zhang 2,† , Jun Li 4 , Suzhen Li 2, Rumei Chen 2 , Shuya Ma 1,3, *,Xiaoqing Liu 2, * and Zuoren Yang. 2025

[4]Heat stress responses mediated by N6-methyladenine DNA methylation in maize. Weijun Guo,Liang Le, Daolei Zhang, Ziwei Wei, Yifan Wang, Yue Wu, Hada Wuriyanghan, Xiaofeng Gu, and Li Pu. 2025

[5]MediaMatch: Prediction of Bacterial Growth on Different Culture Media Using the XGBoost Algorithm. 刘建涵,,许国顺,,刘物格,,刘拓宇,,李彦君,,涂涛,,罗会颖,,伍宁丰,,姚斌,,田健,,张杰,,关菲菲. 2025

作者其他论文 更多>>