数字农科院2.0

Establishment of methods for visual and rapid detection of Nocardia seriolae based on isothermal recombinase polymerase amplification

文献类型： 外文期刊

作者： Liu, Xun;Tan, Shu-Fang;Fan, Wei-Qi;Hao, Yu-Dong;Peng, Qin;Zhang, Yong-An;Zhang, Xu-Jie;Hongshan, Hubei

作者机构：

关键词： （0-1-4）Nocardia seriolae;Detection;Recombinase polymerase amplification;Lateral flow dipstick;Real-time fluorescence

期刊名称： AQUACULTURE

ISSN： 0044-8486

年卷期： 2025 年 598 卷

页码：

收录情况： SCIE（2025版）

摘要： Nocardia seriolae is the main pathogenic bacterium that causes nocardiosis in fish, seriously endangering the healthy and green development of aquaculture. There is an urgent demand in the market for rapid detection methods of N. seriolae. Therefore, in this study, we developed methods for rapid and visual detection of N. seriolae by isothermal recombinase polymerase amplification with lateral flow dipstick (RPA-LFD) and real-time fluorescence RPA based on the conserved sequence of 16S-23S rRNA internal transcribed spacer (ITS). The RPA-LFD and real-time fluorescence RPA methods achieved rapid detection of N. seriolae within 20 min. The minimum detection limit of the two methods for the plasmid pMD18-ITS was 103 copies/mu L, and the minimum detection concentration for the genomic DNA of N. seriolae was 100 pg/mu L. The sensitivity was comparable to that of polymerase chain reaction (PCR), and a high specificity was noted, which can enable the early detection of N. seriolae. In addition, RPA-LFD and real-time fluorescence RPA methods were used to analyze 40 largemouth bass (Micropterus salmoides) samples from fish farms, and the results were consistent with those obtained with conventional PCR. In summary, the methods established in this study are easy to use and exhibit high sensitivity, laying an important foundation for rapid diagnosis of N. seriolae infection.

分类号：

  • 相关文献

[1]Detection method for reverse transcription recombinase-aided amplification of avian influenza virus subtypes H5, H7, and H9. Zongshu Zhang,Zichuang Zhang,Chunguang Wang,Xianghe Zhai,Wenjing Wang,Xi Chen,Tie Zhang. 2024

[2]A novel rapid detection of Senecavirus A using recombinase polymerase amplification (RPA) coupled with lateral flow (LF) dipstrip. Huibao Wang,Jinjie Dong,Tao Zhang,Fan Wang,Rui Yang,Yong Zhang,Xingxu Zhao. 2022

[3]Development and evaluation of a novel visual and rapid detection assay for toxigenic Fusarium graminearum in maize based on recombinase polymerase amplification and lateral flow analysis. Xiaoyan Liang,Xiu Zhang,Hafiz Abdul Haseeb,Tingting Tang,Jihao Shan,Bo Yin,Wei Guo. 2022

[4]Development of recombinase polymerase amplification combined with a lateral flow dipstick assay for rapid and simple detection of Tylenchulus semipenetrans in soil. Song, Zhiqiang,Cheng, Yi,Wang, Tuhong,Wang, Qipei,Qiu, Caisheng,Qiu, Huajiao. 2025

[5]葡萄卷叶相关病毒13在我国葡萄上的首次报道. 范旭东,张尊平,任芳,胡国君,董雅凤. 2019

[6]Highly Sensitive CRISPR/Cas12a-Based Fluorescence Detection of Porcine Reproductive and Respiratory Syndrome Virus. Siyuan Liu,Dagang Tao,Yuying Liao,Yalan Yang,Shouzhang Sun,Yulan Zhao,Peng Yang,Yijie Tang,Bin Chen,Yonggang Liu,Shengsong Xie,Zhonglin Tang. 2021

[7]Highly Sensitive CRISPR/Cas12a-Based Fluorescence Detection of Porcine Reproductive and Respiratory Syndrome Virus. Siyuan Liu,Dagang Tao,Yuying Liao,Yalan Yang,Shouzhang Sun,Yulan Zhao,Peng Yang,Yijie Tang,Bin Chen,Yonggang Liu,Shengsong Xie,Zhonglin Tang. 2021

[8]Development of a rapid on-site detection method for largemouth bass virus based on RPA-CRISPR/Cas12a system. Na Li,Chunmei Dai,Yuelin Mao,Yimeng Zhang,Huiyuan Yan,Huijiao Wang,Yinghui Qin,Baicheng Huang,Xinjie Wang,Lunguang Yao. 2025

[9]Development of a loop-mediated isothermal amplification assay combined with a lateral flow dipstick for rapid and simple detection of classical swine fever virus in the field. Chowdry, Vinay Kumar,Widen, Frederik,Belak, Sandor,Liu, Lihong,Luo, Yuzi,Qiu, Hua-Ji,Shan, Hu. 2014

[10]Establishment and application of a rapid diagnostic method for BVDV and IBRV using recombinase polymerase amplification-lateral flow device. Wang Y.,Shang J.,Li Z.,Zhang A.,Cheng Y.. 2024

[11]Establishment and application of the Recombinase-Aided Amplification-Lateral Flow Dipstick detection method for Pantoea ananatis on rice. Wang, Aiying,Luo, Ju,Wang, Cilin,Hou, Yuxuan,Yang, Baojun,Tang, Jian,Liu, Shuhua. 2023

[12]Rapid detection of avian leukemia virus using CRISPR/Cas13a based lateral flow dipstick. Jing Li,Zichuang Zhang,Zongshu Zhang,Xi Chen,Chunguang Wang,Xianghe Zhai,Tie Zhang. 2024

[13]Rapid Detection of Feline Calicivirus Using Lateral Flow Dipsticks Based on CRISPR/Cas13a System. Zichuang Zhang,Jing Li,Chengqi Zhang,Xue Bai,Tie Zhang. 2024

[14]A Recombinase-Aided Amplification-Lateral Flow Dipstick Detection Technique for Early On-Site Diagnosis of Bacterial Blight Caused by Xanthomonas oryzae pv. oryzae in Rice. Yuxuan Hou,Jie Zhu,Chenglong Lu,Libo Fan,Mengqi Liang,Xiaobo Zhang,Benyi Cheng,Xia Xu,Junyi Gong. 2025

[15]Rapid detection of highly pathogenic porcine reproductive and respiratory syndrome virus by a fluorescent probe-based isothermal recombinase polymerase amplification assay. Yang, Yang,Qin, Xiaodong,Zhang, Zhidong,Sun, Yingjun,Chen, Ting.

[16]Rapid diagnosis of Theileria annulata by recombinase polymerase amplification combined with a lateral flow strip (LF-RPA) in epidemic regions. Yin, Fangyuan,Liu, Junlong,Liu, Aihong,Li, Youquan,Luo, Jianxun,Guan, Guiquan,Yin, Hong,Yin, Hong.

[17]Development of an isothermoal amplification-based assay for rapid visual detection of an Orf virus. Yang, Yang,Qin, Xiaodong,Wang, Guangxiang,Jin, Jiaxin,Shang, Youjun,Zhang, Zhidong,Jin, Jiaxin. 2016

[18]Development of an isothermal recombinase polymerase amplification assay for rapid detection of pseudorabies virus. Yang, Yang,Qin, Xiaodong,Zhang, Wei,Li, Zhiyong,Zhang, Shuaijun,Li, Yanmin,Zhang, Zhidong.

[19]Recombinase Polymerase Amplification Assay for Rapid Field Diagnosis of Stewart’s Wilt of Corn Pathogen Pantoea stewartii subsp. stewartii. Lulu Cai,Qian Tian,Qingqing Meng,Xiaoyang Bao,Peidong Xu,Ji Liu,Wenjun Zhao,Hui Wang. 2023

[20]Simultaneous Detection Of Theileria Annulata A.nd Theileria Orientalis Infections U sing Recombinase Polymerase Amplification. Hassan, MA, Liu, JL, Sajid, MS, Rashid, M, Mahmood, A, Abbas, Q, Guan, GQ, Yin, H, Luo, JX. 2018

作者其他论文 更多>>