数字农科院2.0

A single-dose mRNA vaccine encoding the classical swine fever virus E2-ECD induces durable protective immunity in rabbits

文献类型： 外文期刊

作者： Bian, Li-Jun;Tang, Yu;Yang, Fan;Tian, Hong;Peng, Qin;Tang, Ming-Liang;Chen, Yi-Zhen;Xia, Tian;Li, Shu;Zheng, Hai-Xue;Shu, Hong-Bing;Li, Mi

作者机构：

关键词： Classical swine fever virus (CSFV);mRNA vaccine;E2 protein;protective immunity

期刊名称： VETERINARY RESEARCH

ISSN： 0928-4249

年卷期： 2025 年 56 卷 1 期

页码：

收录情况： SCIE（2025版）

摘要： Classical swine fever virus (CSFV) spreads in domestic and wild pig populations, causing significant economic losses in the swine industry. Despite the global implementation of live attenuated vaccines, CSFV remains a persistent threat, with sporadic outbreaks reported annually. A major limitation of the current vaccines is safety concerns and the inability to differentiate infected from vaccinated animals (DIVA). The development of DIVA-compliant vaccines is desirable for effectively controlling or eradicating classical swine fever (CSF). Here, we developed two lipid nanoparticle (LNP)-encapsulated mRNA vaccines encoding either the extracellular domain of the CSFV envelope protein E2 (E2-ECD) or its N-terminal 172-amino acid fragment (E2-ECD-N). Immunological assays in mice revealed high antigenicity and long-lasting protective antibody responses from a single dose of either the E2-ECD or E2-ECD-N mRNA vaccine. Notably, both the E2-ECD and E2-ECD-N mRNA vaccines induced robust T cell responses in mice. Furthermore, a single dose (100 mu g) of the E2-ECD mRNA vaccine was sufficient to induce long-term (up to 4 months) protective immunity against CSFV infection in rabbits. Our findings highlight the potential of CSFV-E2-based mRNA vaccines as promising strategies for effective CSF prevention and control while enabling DIVA.

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