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Overexpression of GPX2 gene regulates the development of porcine preadipocytes and skeletal muscle cells through MAPK signaling pathway

文献类型： 外文期刊

作者： Chunguang Zhang;Lei Wang;Lei Qin;Yunyan Luo;Zuochen Wen;Akpaca Samson Vignon;Chunting Zheng;Xueli Zhu;Han Chu;Shifan Deng;Liang Hong;Jianbin Zhang;Hua Yang;Jianbo Zhang;Yuhong Ma;Guofang Wu;Chao Sun;Xin Liu;Lei Pu

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期刊名称： PLoS ONE

ISSN： 1932-6203

年卷期： 2024 年 19 卷 5 MAY 期

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收录情况： SCIE（2024版）

摘要： Glutathione peroxidase 2 (GPX2) is a selenium-dependent enzyme and protects cells against oxidative damage. Recently, GPX2 has been identified as a candidate gene for backfat and feed efficiency in pigs. However, it is unclear whether GPX2 regulates the development of porcine preadipocytes and skeletal muscle cells. In this study, adenoviral gene transfer was used to overexpress GPX2. Our findings suggest that overexpression of GPX2 gene inhibited proliferation of porcine preadipocytes. And the process is accompanied by the reduction of the p-p38. GPX2 inhibited adipogenic differentiation and promoted lipid degradation, while ERK1/2 was reduced and p-p38 was increased. Proliferation of porcine skeletal muscle cells was induced after GPX2 overexpression, was accompanied by activation in JNK, ERK1/2, and p-p38. Overexpression methods confirmed that GPX2 has a promoting function in myoblastic differentiation. ERK1/2 pathway was activated and p38 was suppressed during the process. This study lays a foundation for the functional study of GPX2 and provides theoretical support for promoting subcutaneous fat reduction and muscle growth.

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