数字农科院2.0

An Immunocompetent Mongolian Gerbil Model for Hepatitis E Virus Genotype 1 Infection

文献类型： 外文期刊

作者： Liu, Tianxu;He, Qiyu;Yang, Xinyue;Li, Yuebao;Yuan, Disen;Lu, Qinghui;Tang, Tianyu;Guan, Guiwen;Zheng, Liwei;Zhang, He;Xia, Changyou;Yin, Xin;Wei, Guochao;Chen, Xiangmei;Lu, Fengmin;Wang, Lin

作者机构：

关键词： Hepatitis E virus;Mongolian Gerbil;HEV Genotype 1;Small-Animal Model

期刊名称： GASTROENTEROLOGY

ISSN： 0016-5085

年卷期： 2024 年 167 卷 4 期

页码：

收录情况： SCIE（2024版）

摘要： BACKGROUND & AIMS: Hepatitis E virus (HEV), primarily genotype 1 (HEV-1), causes approximately 20.1 million infections, 44,000 deaths, and 3000 stillbirths annually. Current evidence indicates that HEV-1 is only transmitted in humans. Here, we evaluated whether Mongolian gerbils can serve as animal models for HEV-1 infection. METHODS: Mongolian gerbils were used for HEV-1 and hepatitis E virus genotype 3 infection experiments. HEV infection parameters, including detection of HEV RNA and HEV antigen, liver function assessment, and histopathology, were evaluated. RESULTS: We adapted a clinical isolate of HEV-1 for Mongolian gerbils by serial passaging in feces of aged male gerbils. The gerbil- adapted strain obtained at passage 3 induced a robust, acute HEV infection, characterized by stable fecal virus shedding, elevated liver enzymes, histopathologic changes in the liver, and seroconversion to anti-HEV. An infectious complementary DNA clone of the adapted virus was generated. HEV-1-infected - infected pregnant gerbils showed a high rate of maternal mortality and vertical transmission. HEV RNA or antigens were detected in the liver, kidney, intestine, placenta, testis, and fetus liver. Liver and placental transcriptomic analyses indicated activation of host immunity. Tacrolimus prolonged HEV-1 infection, whereas ribavirin cleared infection. The protective efficacy fi cacy of a licensed HEV vaccine was validated using this model. CONCLUSIONS: HEV-1 efficiently fi ciently infected Mongolian gerbils. This HEV-1 infection model will be valuable for investigating hepatitis E immunopathogenesis and evaluating vaccines and antivirals against HEV.

分类号：

  • 相关文献

[1]Seroprevalence of Hepatitis E Virus in Four Species of Parrots in China. Zhang, Xiao-Xuan,Zhang, Nian-Zhang,Zhou, Dong-Hui,Tian, Wei-Peng,Xu, Ying-Tian,Zhu, Xing-Quan,Zhang, Xiao-Xuan,Tian, Wei-Peng,Zhu, Xing-Quan. 2014

[2]Seroprevalence of hepatitis E virus infection in psychiatric patients and control subjects in Shandong Province, eastern China. Cong, Wei,Zhu, Xing-Quan,Cong, Wei,Qian, Ai-Dong,Zhu, Xing-Quan,Meng, Qing-Feng,Li, Bing,Ma, Feng-Ling,Wang, Xiang-Yang,Yu, Chang-Zheng. 2014

[3]Seroprevalence of Hepatitis E Virus Among Pregnant Women and Control Subjects in China. Cong, Wei,Chen, Jia,Zhu, Xing-Quan,Cong, Wei,Qian, Ai-Dong,Zhu, Xing-Quan,Sui, Jian-Chao,Zhang, Xiang-Yan.

[4]Identification of genotype 4 Hepatitis E virus binding proteins on swine liver cells. Zhang, Wen,Hua, Xiuguo,Yang, Shixing,Cui, Li,Zhang, Wen,Shen, Quan,Yin, Hong. 2011

[5]The Genetic Divergences Of Codon U.sage Shed New Lights O n Transmission Of Hepatitis E Virus From Swine To Human. Zhou, JH,Li, XR,Lan, X,Han, SY,Wang, YN,Hu, YH,Pan, QW. 2019

[6]Potential Effects Of Hepatitis E V.irus Infection In Swine O n Public Health In China. Zhou, JH,Shang, YJ,Cao, XA,Wang, YN,Liu, YS,Hu, YH,Lan, X. 2019

[7]Prevalence And Genetic Features Of H.epatitis E Virus In S wine, In Gansu, China. Zhou, JH, Wang, YN, Li, XR, Liu, YS, Pan, Q, Lan, X. 2018

[8]Seroprevalence And Risk Factors Of H.epatitis E Virus Infection I n Cancer Patients In Eastern China. Bai, MJ, Zhou, N, Dong, W, Li, GX, Cong, W, Zhu, XQ. 2018

[9]Virome of high-altitude canine digestive tract and genetic characterization of novel viruses potentially threatening human health. Jiang, Xiaojie,Liu, Jia,Xi, Yuan,Zhang, Qing,Wang, Yongshun,Zhao, Min,Lu, Xiang,Wu, Haisheng,Shan, Tongling,Ni, Bin,Zhang, Wen,Ma, Xiao. 2023

[10]The Viral ORF3 Protein Is Required for Hepatitis E Virus Apical Release and Efficient Growth in Polarized Hepatocytes and Humanized Mice. Sari, Gulce,Zhu, Jingting,Ambardekar, Charuta,Yin, Xin,Boonstra, Andre,Feng, Zongdi,Vanwolleghem, Thomas. 2021

[11]Cellular organelles involved in hepatitis e virus infection. Xing Liu,Menghang Wang,Xin Yin. 2021

[12]Potential zoonotic swine enteric viruses: The risk ignored for public health. Yassein M. Ibrahim,Gebremeskel Mamu Werid,He Zhang,Lizhi Fu,Wei Wang,Hongyan Chen,Yue Wang. 2022

[13]Palmitoylation-dependent association with Annexin II directs hepatitis E virus ORF3 sorting into vesicles and quasi-enveloped virions. Xing Liu,Tianxu Liu,Zhen Shao,Xiaoyan Xiong,Shuhui Qi,Junyong Guan,Menghang Wang,Yan Dong Tang,Zongdi Feng,Lin Wang,Xin Yin. 2025

[14]Cell binding tropism of rat hepatitis E virus is a pivotal determinant of its zoonotic transmission to humans. Hongbo Guo,Jiaqi Xu,Jianwen Situ,Chunyang Li,Xia Wang,Yao Hou,Guangde Yang,Lingli Wang,Dong Ying,Zheng Li,Zijie Wang,Jia Su,Yibo Ding,Dou Zeng,Jikai Zhang,Xiaohui Ding,Shusheng Wu,Weiwei Miao,Renxian Tang,Yihan Lu,Huihui Kong,Peng Zhou,Zizheng Zheng,Kuiyang Zheng,Xiucheng Pan,Siddharth Sridhar,Wenshi Wang. 2024

[15]Decoding the role of the intestinal epithelium in hepatitis E virus infection using a human organoid prototype of “gut-liver” axis. Kuan Liu,Yang Wang,Jiangrong Zhou,Joy Joàn van der Meij,Luc J.W. van der Laan,Pengfei Li,Qiuwei Pan. 2025

[16]Hepatitis E virus infects human testicular tissue and Sertoli cells. Tianxu Liu,Yalei Cao,Jiaming Weng,Songzhan Gao,Zirun Jin,Yun Zhang,Yuzhuo Yang,He Zhang,Changyou Xia,Xin Yin,Yong Luo,Qiyu He,Hui Jiang,Lin Wang,Zhe Zhang. 2024

[17]Optimization of immunosuppression strategies for the establishment of chronic hepatitis E virus infection in rabbits. He, Qiyu,Liu, Tianxu,Yang, Xinyue,Yuan, Disen,Lu, Qinghui,Li, Yuebao,Zhang, He,Liu, Xing,Xia, Changyou,Sridhar, Siddharth,Tian, Lili,Liu, Xiaofeng,Meng, Lulu,Ning, Jing,Lu, Fengmin,Wang, Ling,Yin, Xin,Wang, Lin. 2024

[18]Hepatitis E Virus Detection in Swine Livers and Feces in Heilongjiang, Northeastern China. He, Haijuan,Li, Hai,Yan, Lei,Wang, Gang,Liu, Yonggang,An, Tongqing,Tu, Yabin,Wang, Shujie,Cai, Xuehui. 2025

作者其他论文 更多>>