数字农科院2.0

Advancing High-Throughput MS-Based Protein Quantification: A Case Study on Quantifying 10 Major Food Allergens by LC-MS/MS Using a One-Sample Multipoint External Calibration Curve

文献类型： 外文期刊

作者： Yang, Shupeng;Lin, Haopeng;Yang, Peijie;Meng, Junhong;Abdallah, Mohamed F.;Shencheng, Yingnan;Li, Ruohan;Li, Jianxun;Liu, Shuyan;Li, Qianqian;Lu, Peng;Zhang, Rong;Li, Yi

作者机构：

关键词： food allergen;LC-MS/MS;calibration curve;quantitative analysis;food safety

期刊名称： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

ISSN： 0021-8561

年卷期： 2024 年

页码：

收录情况： SCIE（2024版） ; ; EI（2024版）

摘要： The LC-MS-based method has emerged as the preferred approach for quantifying food allergens. However, the preparation of a traditional calibration curve (MSCC) is labor-intensive and error-prone. Here, a sensitive and robust LC-MS/MS method for quantifying 10 major food allergens was developed and validated, where the one-sample multipoint external calibration curve (OSCC) was employed instead of MSCC. By employing the multiple isotopologue reaction monitoring (MIRM) technique with only one spiked level in the blank, OSCC can be effectively established. Results demonstrate that the proposed method exhibits excellent performance in selectivity, sensitivity, accuracy, and precision, comparable to that of the traditional MSCC. Additionally, this strategy allows for isotope sample dilution by monitoring the less abundant MIRM channel. Moreover, the developed method was successfully applied to investigate the contamination of 10 food allergens in commercial food products. With its high throughput and robustness, the MIRM-OSCC-LC-MS/MS methodology has many potential applications, especially in the MS-based protein quantification analysis.

分类号：

  • 相关文献

[1]One sample multi-point calibration curve as a novel approach for quantitative LC-MS analysis: The quantitation of six aflatoxins in milk and oat-based milk as an example. Feifei Sun,Peixu Wu,Mohamed F. Abdallah,Haiguang Tan,Yi Li,Shupeng Yang. 2023

[2]Integrating a Multiple Isotopologue Reaction-Monitoring Technique and LC-MS/MS for Quantitation of Small Molecules: Ten Mycotoxins in Cereals as an Example. Feifei Sun,Yanshen Li,Haiguang Tan,Peixu Wu,Yingnan Shencheng,Haopeng Lin,Peng Lu,Rong Zhang,Shuyan Liu,Yi Li,Shupeng Yang. 2024

[3]Development and Validation of a QconCAT-Based LC-MS/MS Method for the Quantification of 12 Food Allergens in Foods. Shencheng, Yingnan,Zhou, Meizhen,Zhao, Yumeng,Li, Siyi,Lin, Haopeng,Abdallah, Mohamed F.,Zhang, Rong,Liu, Shuyan,Li, Yi,Yang, Shupeng. 2025

[4]The dissipation, processing factors, metabolites, and risk assessment of pesticides in honeysuckle from field to table. Ouli Xiao,Minmin Li,Deyong Chen,Jieyin Chen,Jesus Simal-Gandara,Xiaofeng Dai,Zhiqiang Kong. 2022

[5]Development and validation of an LC-MS/MS method for a sensitive quantitation of livestock and poultry meat allergens. Shupeng Yang,Yingnan Shencheng,Yumeng Zhao,Meizhen Zhou,Mohamed F. Abdallah,Yanan Gao,Min Gao,Rong Zhang,Shuyan Liu,Yi Li. 2025

[6]An integrated calibration strategy for the development and validation of an LC-MS/MS method for accurate quantification of egg allergens (Gal d 1-6) in foods. Yang, Shupeng,Chen, Jingjing,Abdallah, Mohamed F.,Lin, Haopeng,Yang, Peijie,Li, Jianxun,Zhang, Rong,Li, Qianqian,Lu, Peng,Liu, Shuyan,Li, Yi. 2024

[7]阿维菌素在柑桔和土壤中的残留及其消解动态. 张娇娇,刘炎,叶玉凤,赵其阳,彭震宇,焦必宁,王成秋. 2017

[8]基于分散固相萃取液相色谱-串联质谱法测定大米中8种真菌毒素. 孙伟华,杨欢,曹赵云,马有宁,秦美玲,柴爽爽,陈铭学. 2017

[9]大丽轮枝菌分泌蛋白提取方法比较. 肖红利,桂月晶,祁伟彦,陈捷胤,李蕾,徐明,戴小枫. 2014

[10]LC-MS/MS检测饲料中二甲氧苄啶、甲氧苄啶和二甲氧甲基苄啶方法的建立. 杨亚军,刘希望,李冰,孔晓军,李世宏,秦哲,杜文斌,李剑勇. 2015

[11]黄瓜根分泌物中的7;8-苯并黄酮及其对枯萎病发生的影响. 李世东,郭荣君. 2010

[12]液相色谱-串联质谱法检测鸡蛋中金刚烷胺残留. 汤晓艳,陈东宇,周剑,毛雪飞,邱静,王敏. 2014

[13]晋麦47幼苗中一个水分胁迫应答蛋白的SDS-PAGE和Nano LC-MS/MS鉴定. 范锋贵,张晓科,任万杰,王晓龙,叶石,付晓洁,王宏礼,朱建楚. 2012

[14]黄瓜根分泌物中的7,8-苯并黄酮及其对枯萎病发生的影响. 李世东,郭荣君. 2010

[15]玉米DDGS中抗生素残留分析. 姚婷,秦玉昌,吴宁鹏,彭丽,孙丹丹,谷旭. 2014

[16]荷斯坦奶牛瘤胃微生物脲酶的分离与鉴定. 赵圣国,王加启,刘开朗,李旦,于萍,卜登攀. 2010

[17]卷丹百合及其鳞茎中秋水仙碱含量的测定. 陈凯,王米,何菁琦,张赛奇,许璟. 2020

[18]利用多功能针式过滤器快速检测油菜和苹果中68种农药及代谢物残留. 贾琪,王纯强,马蕊,王宇彤,钱永忠,邱静. 2019

[19]农产品与饲料中T-2毒素免疫亲和柱净化-液相色谱串联质谱检测技术研究. 李静,马飞,李培武,张奇,丁小霞,张文. 2014

[20]LC-MS/MS测定青稞中氨基酸的含量及主成分分析. 王猛强,邱城,张唐伟,李腾飞,李壮,王淼,曹振,王珊珊,郑鹭飞,金茂俊,王静,邵华,金芬,佘永新. 2022

作者其他论文 更多>>