数字农科院2.0

Biosynthesis of bridged tricyclic sesquiterpenes in Inula lineariifolia

文献类型： 外文期刊

作者： Wu, Yingmei;Chen, Xueqing;Hao, Fuhua;Liu, Yu;Luo, Wei;Zhu, Yaru;Li, Li;Han, Fei;Zhang, Yunluo;Jiang, Ying;Xiong, Xingyao;Ro, Dae-Kyun;Shang, Yi;Huang, Sanwen;Gou, Junbo

作者机构：

关键词： （2-0-4）Presilphiperfolane-type sesquiterpenes;tricyclic sesquiterpene synthase;Inula lineariifolia;biological activity;Nigrospora oryzae

期刊名称： PLANT JOURNAL

ISSN： 0960-7412

年卷期： 2024 年

页码：

收录情况： SCIE（2024版） ; ; EI（2024版）

摘要： Presilphiperfolane-type sesquiterpenes represent a unique group of atypical sesquiterpenoids characterized by their distinctive tricyclic structure. They have significant potential as lead compounds for pharmaceutical and agrochemical development. Herein, we utilized a transcriptomic approach to identify a terpene synthase (TPS) gene responsible for the biosynthesis of rare presilphiperfolane-type sesquiterpenes in Inula lineariifolia, designated as IlTPS1. Through phylogenetic analysis, we have identified the evolutionary conservation of key motifs, including RR(x)8W, DDxxD, and NSE/DTE in IlTPS1, which are shared with other tricyclic sesquiterpene synthases in the TPS-a subfamily of Asteraceae plants. Subsequent biochemical characterization of recombinant IlTPS1 revealed it to be a multiproduct enzyme responsible for the synthesis of various tricyclic sesquiterpene alcohols from farnesyl diphosphate (FPP), resulting in production of seven distinct sesquiterpenes. Mass spectrometry and nuclear magnetic resonance (NMR) spectrometry identified presilphiperfolan-8 beta-ol and presilphiperfol-7-ene as predominant products. Furthermore, biological activity assays revealed that the products from IlTPS1 exhibited a potent antifungal activity against Nigrospora oryzae. Our study represents a significant advancement as it not only functionally identifies the first step enzyme in presilphiperfolane biosynthesis but also establishes the heterologous bioproduction of these unique sesquiterpenes.

分类号：

  • 相关文献

[1]Genome sequence resource for nigrospora oryzae, an important pathogenic fungus threatening crop production. Yafei Wang,Jinai Yao,Zhiqiang Li,Jianfei Huo,Shaoqun Zhou,Wende Liu,Hanxiang Wu. 2021

[2]Genome sequence resource for nigrospora oryzae, an important pathogenic fungus threatening crop production. Yafei Wang,Jinai Yao,Zhiqiang Li,Jianfei Huo,Shaoqun Zhou,Wende Liu,Hanxiang Wu. 2021

[3]First Report of Leaf Spot Disease Caused by Nigrospora oryzae on Nicotiana tabacum in China. Dongkun Wang,Yichi Li,Guangjun Sun,Yongfeng Ai,Fenglong Wang,Xiaoqiang Wang. 2022

[4]Synthesis, Crystal Structure and Biological Activity of N-tert-butyl-N-(4-methyl-1,2,3-thiadiazole)-5-yl-N '-(4-methyl-1,2,3-thiadiazole)-5-formyl-N '-3,5-dichloropyridin-2-yl-diacylhydrazine. Wang Huan,Fu Yi-Feng,Fan Zhi-Jin,Song Hai-Bin,Wu Qing-Jun,Zhang You-Jun,Belskaya, N. P.,Bakulev, V. A.. 2011

[5]Synthesis, Crystal Structure and Biological Activity of Novel Anthranilic Diamides Containing 1,2,3-Thiadiazole. Dong, Wei-Li,Xu, Jun-Ying,Liu, Xing-Hai,Li, Zheng-Ming,Li, Bao-Ju,Shi, Yan-Xia. 2008

[6]Synthesis and biological activities of 1,2,3-thiadiazol-4-acetamide (Acetmorpholide) derivatives. Dong Wei-Li,Yao Hong-Wei,Wang Feng-Long,Li Zheng-Ming,Shen Li-Li,Qian Yu-Mei,Zhao Wei-Guang. 2007

[7]Chemical Constituents and Biological Activities of Plants from the Genus Ligustrum. She, Gai-Mei,Gao, Bei-Bei,She, Dong-Mei. 2013

[8]Synthesis of E-beta-farnesene analogues containing five-membered azaheterocycles and their biological activity. Ling Yun,Yang Xin-Ling,Chen Fu-Heng,Rui Chang-Hui,Fan Xian-Lin. 2008

[9]Secondary Metabolites from the Marine Algal-Derived Endophytic Fungi: Chemical Diversity and Biological Activity. Zhang, Peng,Li, Xin,Wang, Bin-Gui,Zhang, Peng.

[10]Effects Of Different Modification Techniques O.n Molecular Structure And B ioactivity Of Bombyx Mori Pupa Protein. Ren, Zi-Xu,Yu, Hai-Yan,Zhou, Zhi-Feng,Jia, Jun-Qiang,Gui, Zhong-Zheng,Gui, Zhong-Zheng,Jia, Jun-Qiang. 2017

[11]Interferon-omega: Current status in clinical applications. Li, Shi-fang,Zhao, Fu-rong,Shao, Jun-jun,Xie, Yin-li,Chang, Hui-yun,Zhang, Yong-guang.

[12]Synthesis and Biological Activity of Novel Furan/Thiophene and Piperazine-Containing (Bis)1,2,4-triazole Mannich Bases. Wang, Baolei,Zhan, Yizhou,Zhang, Liyuan,Zhang, Yan,Wang, Lizhong,Zhang, Xiao,Li, Yonghong,Li, Zhengming,Shi, Yanxia,Li, Baoju. 2015

[13]Establishment of a stable CHO cell line with high level expression of recombinant porcine IFN-beta. Chen, Weiye,Hu, Qianqian,Qu, Linmao,Li, HaoBo,Huang, Kehe,Chen, Weiye,Cao, Wenyan,Zhao, Huijun,Hu, Qianqian,Qu, Linmao,Hu, Sen,Ge, Jinying,Wen, Zhiyuan,Wang, Xijun,Li, HaoBo,Bu, Zhigao. 2011

[14]Expression of biologically active recombinant equine interferon-gamma in Escherichia coli. Bai, Yu,Tong, Tiegang,Liu, Guangliang,Chen, Weiye,Zhang, Weijun,Wang, Qun,Yang, Tao,Bu, Zhigao,Wu, Donglai,Bai, Yu. 2010

[15]Synthesis and biological activity of novel benzimidazole derivatives as potential antifungal agents. Zhang, Tao,She, Dongmei,Ning, Jun,Mei, Xiangdong,Li, Yaofa,Pan, Wenliang,Gao, Zhanlin.

[16]Biological Characteristics of Recombinant Arthrobotrys oligospora Chitinase AO-801. Gong S.,Meng Q.,Qiao J.,Huang Y.,Zhong W.,Zhang G.,Zhang K.,Li N.,Shang Y.,Li Z.,Cai X.. 2022

[17]Humic Acid Modified by Being Incorporated Into Phosphate Fertilizer Increases Its Potency in Stimulating Maize Growth and Nutrient Absorption. Jianyuan Jing,Shuiqin Zhang,Liang Yuan,Yanting Li,Chengrong Chen,Bingqiang Zhao. 2022

[18]Exploration on bioactive properties of quinoa protein hydrolysate and peptides: a review. Guo, Huimin Hao, Yuqiong Yang, Xiushi Ren, Guixing Richel, Aurore. 2021

[19]Structural characteristics and biological activities of a pectic-polysaccharide from okra affected by ultrasound assisted metal-free Fenton reaction. Ding Tao Wu,Yuan He,Meng Xi Fu,Ren You Gan,Yi Chen Hu,Lian Xin Peng,Gang Zhao,Liang Zou. 2022

[20]Microwave-Assisted Deep Eutectic Solvent Extraction, Structural Characteristics, and Biological Functions of Polysaccharides from Sweet Tea (Lithocarpus litseifolius) Leaves. Wu D.-T.,Fu M.-X.,Guo H.,Hu Y.-C.,Zheng X.-Q.,Gan R.-Y.,Zou L.. 2022

作者其他论文 更多>>