数字农科院2.0

Functional Analysis Of A Viroid R.na Motif Mediating Cell-To-Cell M ovement In Nicotiana Benthamiana

文献类型： 外文期刊

作者： Jiang, Dongmei;Li, Shifang;Wang, Meng;Jiang, Dongmei;Jiang, Dongmei

作者机构：

关键词： viroid; function; RNA; motif; movement

期刊名称： JOURNAL OF GENERAL VIROLOGY

ISSN： 0022-1317

年卷期： 2017 年 98 卷 1.0 期

页码：

收录情况： JCR（2021版）

摘要： Cell-to-cell trafficking through different cellular layers is a key process for various RNAs including those of plant viruses and viroids, but the regulatory mechanisms involved are still not fully elucidated and good model systems are important. Here, we analyse the function of a simple RNA motif (termed 'loop19') in potato spindle tuber viroid (PSTVd) which is required for trafficking in Nicotiana benthamiana leaves. Northern blotting, reverse transcriptase PCR (RT-PCR) and in situ hybridization analyses demonstrated that unlike wild-type PSTVd, which was present in the nuclei in all cell types, the trafficking-defective loop19 mutants were visible only in the nuclei of upper epidermal and palisade mesophyll cells, which shows that PSTVd loop19 plays a role in mediating RNA trafficking from palisade to spongy mesophyll cells in N. benthamiana leaves. Our findings and approaches have broad implications for studying the RNA motifs mediating trafficking of RNAs across specific cellular boundaries in other biological systems.

分类号：

  • 相关文献

[1]Evolution Of Sex Pheromone Receptors In Dendrolimus Punctatus Walker (Lepidoptera: Lasiocampidae) Is Divergent From Other Moth Species. Shen, SF, Cao, S, Zhang, Z, Kong, XB, Liu, F, Wang, GR, Zhang, SF. 2020

[2]Quantitative, Convenient, and Efficient Genome-Wide R-Loop Profiling by ssDRIP-Seq in Multiple Organisms. Xu W., Li K., Li Q., Li S., Zhou J., Sun Q.. 2022

[3]Transient Expression–Mediated Gene Silencing in Plants and Suppression of Gene Silencing with Viral Suppressors. Li F., Wang A.. 2022

[4]Purification of Total RNAs and Small RNAs from Fruit Tree Leaf Tissues. Ma Y., Li S.. 2022

[5]Plant Volatiles Modulate Seasonal Dynamics Between Hosts Of The Polyphagous Mirid Bug Apolygus Lucorum. Pan, HS, Xiu, CL, Williams, L, Lu, YH. 2021

[6]Systematic Analysis Of The Basic/Helix-Loop-Helix (Bhlh) Transcription Factor Family In Pummelo (Citrus Grandis) And Identification Of The Key Members Involved In The Response To Iron Deficiency. Hui, Qiu-Ling,Qiu, Jie-Ya,Zhang, Xiao-Yong,Xu, Yuan-Yuan,Fu, Xing-Zheng,Peng, Liang-Zhi,He, Yi-Zhong,Hui, Qiu-Ling,Qiu, Jie-Ya,Zhang, Xiao-Yong,Xu, Yuan-Yuan,Fu, Xing-Zheng,Peng, Liang-Zhi,He, Yi-Zhong. 2020

[7]Evolutionarily Informed Deep Learning Methods F.or Predicting Relative Transcript A bundance From Dna Sequence. Washburn, Jacob D.,Wang, Hai,Wang, Hai,Wang, Hai,Valluru, Ravi,Ramstein, Guillaume,Mejia-Guerra, Maria Katherine,Kremling, Karl A.,Wang, Hai,Buckler, Edward S.,Buckler, Edward S.. 2019

[8]Aflatoxin M1 and ochratoxin A induce a competitive endogenous RNA regulatory network of intestinal immunosuppression by whole-transcriptome analysis. Gao Y.-N., Wang Z.-W., Yang X., Wang J.-Q., Zheng N.. 2023

[9]A deletion variant within the FGF5 gene in goats is associated with gene expression levels and cashmere growth. Li Y., Song S., Zhang Z., Liu X., Zhang Y., Guangxin E., Ma Y., Jiang L.. 2022

[10]Physiological and transcriptomic analyses of brassinosteroid function in kiwifruit root. Wu Z., Gu S., Gu H., Cheng D., Li L., Guo X., Wang M., He S., Li M., Chen J.. 2022

[11]Emerging Argonaute-based nucleic acid biosensors. Qin Y., Li Y., Hu Y.. 2022

[12]OsSPL14 acts upstream of OsPIN1b and PILS6b to modulate axillary bud outgrowth by fine-tuning auxin transport in rice. Li Y., He Y., Liu Z., Qin T., Wang L., Chen Z., Zhang B., Zhang H., Li H., Liu L., Zhang J., Yuan W.. 2022

[13]Validation of Suitable Reference Genes for Gene Expression Studies on Yak Testis Development. Yan, Ping,Bao, Pengjia,Chu, Min,Xiong, Lin,Zhou, Xuelan,Ding, Xuezhi,Pei, Jie,Wu, Xiaoyun,Guo, Xian,Liang, Chunnian. 2020

[14]Environmental level of bisphenol F induced reproductive toxicity toward zebrafish. Mu X., Qi S., Liu J., Wang H., Yuan L., Qian L., Li T., Huang Y., Wang C., Guo Y., Li Y.. 2022

[15]Molecular Characterisation And Functional Analysis O.f A Cytochrome P450 G ene In Cotton. Sun, Quan,Wang, Weina,Shi, Yuzhen,Sun, Quan,Wang, Weina,Zhang, Xiao,Zhou, Kexue,Yuan, Youlu,Zhang, Xiangrui,Mo, Jianchuan,Cheng, Jieru,Chu, Zongyan,Cai, Chaowei,Cai, Yingfan,Du, Xiongming,Long, Lu,Miao, Chen,Gao, Wei,Liu, Yujia. 2017

[16]Gbabr1 Is Associated With Verticillium W.ilt Resistance In Cotton. Shi, Yuzhen,Qin, Runkuan,Long, Lu,Zhang, Yuanyuan,Mo, Jianchuan,Cai, Yingfan,Cheng, Jieru,Chu, Zongyan,Liu, Yujia,Long, Lu,Wang, Weina,Wang, Weina,Liu, Xin,Li, Bo,Wang, Chenxiao,Xie, Yuanhui,Sun, Quan,Yuan, Youlu,Xu, Lingling. 2018

[17]Molecular Characterization And Expression Profile O.f Nanos In Schistosoma J aponicum And Its Influence On The Expression Several Mammalian Stem Cell Factors. Giri, BR, Du, XL, Xia, TQ, Chen, YJ, Li, H, Cheng, GF. 2017

[18]Nine Facultative Endosymbionts In Aphids. A. Review. Chen, Julian,Wang, Zhenying,Hatt, Severin,Hatt, Severin,Guo, Jianqing,Guo, Jianqing,Hatt, Severin,He, Kanglai,Francis, Frederic,Guo, Jianqing. 2017

[19]Current Knowledge Of Detoxification Mechanisms O.f Xenobiotic In Honey B ees. Gong, YH, Diao, QY. 2017

[20]Solanesol Biosynthesis In Plants .. Yan, N, Liu, YH, Zhang, HB, Du, YM, Liu, XM, Zhang, ZF. 2017

作者其他论文 更多>>