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Trypsinized Cry1Fa And Vip3Aa Have N.o Detrimental Effects On T he Adult Green Lacewing Chrysopa Pallens (Neuroptera: Chrysopidae)

文献类型： 外文期刊

作者： Ejaz, Samina;Iqbal, Muhammad;Zhang, Shuai;Ali, Intazar;Ali, Intazar;Cui, Jin-jie

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关键词： Ecological risk assessment; Bacillus thuringiensis proteins; Double-antibody sandwich enzyme-linked immunosorbent assay; Insect pest management; Genetic engineering

期刊名称： APPLIED ENTOMOLOGY AND ZOOLOGY

ISSN： 0003-6862

年卷期： 2017 年 52 卷 2 期

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摘要： An exposure bioassay was established for green lacewing Chrysopa pallens (Rambur) (Neuroptera: Chrysopidae) adults using a suitable artificial diet and honey-water solution (20%) to assess the toxicity of trypsinized Cry1Fa and Vip3Aa. Lethal and sub-lethal life table parameters were unaffected after C. pallens adults were given honey-water solutions containing Cry1Fa and Vip3Aa (50 mu g/ml) and an artificial diet. In contrast, life table parameters of C. pallens adults were significantly affected when boric acid was mixed with the honey-water solution as a positive control. The uptake, temporal stability and bioactivity of Cry1Fa and Vip3Aa before and after C. pallens access to honey-water were confirmed using double antibody sandwich enzyme-linked immunosorbent assays and a bioactivity verification bioassay. The results prove that trypsinized Cry1Fa and Vip3Aa are safe for C. pallens adults, thus it is speculated that transgenic crops expressing Cry1Fa and Vip3Aa have no detrimental effects on lacewings and are compatible with biological control programs. This study describes a robust experimental design for evaluating the potential toxicity of alkaline gut-activated Bacillus thuringiensis (Bt) proteins on C. pallens adults which can be used to determine the potential toxicity of other Bt proteins on this species.

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