Molecular Cloning And Characterization Of H.igh Mobility Group Box ( Hmgb) Gene From Beauveria Bassiana- Infected Silkworm, Bombyx Mori
文献类型: 外文期刊
作者: Ruilin, L.;Han, W.;Ruilin, L.;Xijie, G.;Xijie, G.;Han, W.;Chengxiang, H.;Chengxiang, H.;Chengxiang, H.;Dingding, L.;Dingding, L.
作者机构:
关键词: Bombyx mori; Beauveria bassiana; High Mobility Group box protein; rapid amplification of cDNA ends; qRT-PCR
期刊名称: ISJ-INVERTEBRATE SURVIVAL JOURNAL
ISSN: 1824-307X
年卷期: 2017 年 14 卷
页码:
摘要: The cDNA sequence of High Mobility Group box ( HMGB) from Bombyx mori was cloned by rapid amplification of cDNA ends and submitted to GenBank ( the accession number was JF969272). The full-length cDNA of BmHMGB included 3015 bp, with five exons and four introns. It contained a 313 bp 5' UTR, a 2114 bp 3' UTR with a polyadenylation signal sequence AATAAA and a poly( A) tail. It encodes a 195- amino acid polypeptide with a predicted molecular weight about 22991.9Da and a theoretical isoelectric point 10.00. Phylogenetic analysis revealed that BmHMGB is grouped in insect HMGB proteins. BmHMGB was mainly expressed in hemolymph, cuticles, midgut and fat body by RT-PCR. In these tissues, the relative expression of BmHMGB with Beauveria bassiana infected silkworm was higher than the normal ones by fluorescent quantitative real-time PCR. Therefore, BmHMGB may play an important function in the response to B. bassiana infection of silkworms.
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