数字农科院2.0

Development Of A Sensitive And R.eliable Droplet Digital Pcr A ssay For The Detection Of 'Candidatus Liberibacter Asiaticus'

文献类型： 外文期刊

作者： Liu Xue-lu;Lou Bing-hai;Zhou Chang-yong;Zhong Xi;Wang Xue-feng

作者机构：

关键词： citrus Huanglongbing; early diagnosis; droplet digital PCR

期刊名称： JOURNAL OF INTEGRATIVE AGRICULTURE

ISSN： 2095-3119

年卷期： 2018 年 17 卷 2 期

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摘要： Citrus Huanglongbing (HLB, yellow shoot disease) is one of the most serious citrus diseases worldwide. To better improve the detection sensitivity, a droplet digital PCR (ddPCR) assay was developed for the rapid detection of 'Candidatus Liberibacter asiaticus' (Las), the putative causal agent of HLB. The detection of sensitivity comparison using positive plasmid indicated that ddPCR was superior to quantitative PCR (qPCR) for detecting and quantifying Las at low concentrations. The Las detection of 40 field samples also showed that six of 13 asymptomatic samples (46.15%) with high C-t value (> 35) were positive by ddPCR. This methodology showed great potential for early HLB infection diagnosis.

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