数字农科院2.0

Complete genome sequence analysis of two Citrus tatter leaf virus (CTLV) isolates from China

文献类型： 外文期刊

作者： Song Zhen;Li Zhong-an;Liu Ke-hong;Zhou Chang-yong

作者机构：

关键词： Citrus tatter leaf virus;Apple stem grooving virus;phylogenetic analysis;genome

期刊名称： JOURNAL OF INTEGRATIVE AGRICULTURE

ISSN： 2095-3119

年卷期： 2015 年 14 卷 5 期

页码：

收录情况： SCI

摘要： In order to understand molecular characterization of Citrus tatter leaf virus (CTLV) isolated from China, full-length cDNAs of CTLV-MTH and CTLV-XHC from Citrus reticulata and Citrus sinensis were cloned and sequenced based on whole-genome amplification by RT-PCR. The complete nucleotide sequences of CTLV-MTH and CTLV-XHC were determined to be 6497 nucleotides in length and shared 79.9-91.0% and 78.8-98.0% nucleotide sequence identity, respectively, with other Apple stem grooving virus (ASGV) or CTLV strains available in GenBank. Unexpectedly, CTLV-MTH showed the highest nucleotide sequence identity (91%) with an apple isolate of ASGV, followed by 86.5% with ASGV-HH and 85.7% with ASGV-CHN. Furthermore, CTLV-MTH and three ASGV strains were grouped to a separate cluster in the phylogenetic tree, suggesting it has a closer relationship to ASGV than to CTLV. Therefore, it can be concluded roughly that CTLV may be not a distinct strains of ASGV. We proposed that Citrus tatter leaf virus should be renamed Apple stem grooving virus.

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