数字农科院2.0

Functional Characterization Of (E)-Beta-Caryophyllene Synthase F.rom Lima Bean And I ts Up-Regulation By Spider Mites And Alamethicin

文献类型： 外文期刊

作者： Wang Gui-rong;Li Feng-qi;Fu Ning-ning;Li Feng-qi;Zhou Jing-jiang

作者机构：

关键词： plant defence; ferpene synthases; (E)-beta-caryophyllene; lima bean

期刊名称： JOURNAL OF INTEGRATIVE AGRICULTURE

ISSN： 2095-3119

年卷期： 2017 年 16 卷 10.0 期

页码：

收录情况： JCR（2021版） ; CSCD（2020-2021年度） ; 农林核心（2020版） ; 科技核心（2020版）

摘要： (E)-beta-Caryophyllene is a sesquiterpene compound widely distributed in plants and functions in plant defence. However, little is known about the sequence and function of (E)-beta-caryophyllene synthase in lima bean (Phaseolus lunatus). Here, we report a new full-length cDNA (PICAHS) encoding (E)-beta-caryophyllene synthase, a possible key enzyme of plant defence. The cDNA of PICAHS contains an open reading frame of 1761 bp, encoding a protein of 586 amino acids with a predicted mass of 67.95 kDa. The deduced amino acid sequence shows 52% identity with sesquiterpene synthase MtCAHS of Medicago truncatula. Based on phylogenetic analysis, PICAHS is classified as the terpene synthases (TPS)-a subfamily. The recombinant enzyme, expressed in Escherichia coli, catalysed the formation of a major product (E)-beta-caryophyllene (82%) and a minor product a-humulene (18%) from farnesyl diphosphate. Real-time quantitative PCR (qRT-PCR) analysis found that the PICAHS transcript was significantly up-regulated in leaves after treatment with spider mites and alamethicin (ALA), suggesting its ecological function in plant defence.

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