数字农科院2.0

Evidence of the involvement of a plus-c odorant-binding protein hparobp14 in host plant selection and oviposition of the scarab beetle holotrichia parallela

文献类型： 外文期刊

作者： Qu Yafei;Liu Xiangyu;Zhao Xu;Qin Jianhui;Cao Yazhong;Li Kebin;Zhou Jing-Jiang;Yin Jiao;Wang Senshan

作者机构：

关键词： Behavior response; EAG recording; Fluorescence competitive binding; Holotrichia parallela; Odorant-binding protein; RNA interference; Scarab beetle

期刊名称： Insects

ISSN： 2075-4450

年卷期： 2021 年 12 卷 5 期

页码：

收录情况： JCR（2021版）

摘要： Holotrichia parallela is one of the agriculturally important scarab beetle pests in China. In this study, HparOBP14 was cloned, which is the most abundantly expressed among the OBP genes in the legs of female H. parallela adults. Sequence comparison and phylogenetic analysis showed that HparOBP14 has a Plus-C structure motif. The expression profile analysis revealed that HparOBP14 expression was the highest in the female antennae and then in the legs. The fluorescence competitive binding experiment of the recombinant HparOBP14 protein showed that HparOBP14 had an affinity with 6-methyl-5-heptene-2-one (plant volatile), 3-methylindole, p-cymene, methanol, formaldehyde, α-pinene, and geraniol (organic fertilizer volatile). Knockdown HparOBP14 expression decreased significantly the EAG response of the injected female adults to p-cymene, methanol, formaldehyde, α-pinene, and geraniol. Similarly, the injected female adults were significantly less attracted to geraniol and methanol. Therefore, HparOBP14 might bind organic matter volatiles during oviposition. These results are not only helpful to analyze the olfactory recognition mechanism of female adult H. parallela when choosing suitable oviposition sites, but also to provide target genes for green prevention and control of H. parallela in the future. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

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